WorldCat Identities

Clarke, Alan R.

Overview
Works: 2 works in 15 publications in 1 language and 815 library holdings
Genres: Handbooks, manuals, etc 
Roles: Editor
Classifications: QH442.6, 576.50724
Publication Timeline
Key
Publications about  Alan R Clarke Publications about Alan R Clarke
Publications by  Alan R Clarke Publications by Alan R Clarke
Most widely held works by Alan R Clarke
Transgenesis techniques principles and protocols by Alan R Clarke ( )
13 editions published between 2001 and 2002 in English and held by 813 WorldCat member libraries worldwide
The past decade has witnessed a spectacular explosion in both the development and the use of transgenic technologies. In Transgenesis Techniques: Principles and Protocols, Second Edition, authoritative researchers-including members of the Dolly team-take stock of these exciting developments to present a collection of readily reproducible techniques for the generation and analysis of transgenic animals, focusing on the manipulation of the mammalian genome. This second edition devotes much space to the creation of genetically modified murine strains, providing access to the germline both by conventional pronuclear injection and by retroviral and adenoviral infection. Extensive coverage is also given to the generation, maintenance, and manipulation of embryonic stem cell lineages, with protocols for both constitutive and conditional (Cre-lox or the Flp-frt systems) gene targeting. Additional chapters provide cutting-edge techniques for the cryopreservation of both male and female germlines, for the generation of transgenic sheep by nuclear transfer, and for the basic analysis of transgenic organisms. Comprehensive and highly practical, this second edition of Transgenesis Techniques: Principles and Protocols updates and expands the acclaimed first edition with a wealth of easy-to-follow cutting-edge protocols and practical tips for producing transgenic animals and analyzing their integrated transgenes
Transgenesis Techniques Principles and Protocols. Methods in Molecular Biology, Volume 180 ( )
2 editions published in 2002 in English and held by 2 WorldCat member libraries worldwide
This revised and updated edition of a much-acclaimed classic presents a collection of readily reproducible techniques for the generation and analysis of transgenic animals, with emphasis on the manipulation of the mammalian genome. It concentrates on the creation and maintenance of genetically modified murine strains, providing access to the germline by both conventional pronuclear injection, and by retroviral and adenoviral infection. Extensive coverage is also given to the generation, maintenance, and manipulation of embryonic stem cell lineages, with protocols for both constitutive and conditional (Cre-lox or the Flp-frt systems) gene targeting. Additional chapters provide cutting-edge techniques for the cryopreservation of both male and female germlines, for the generation of transgenic sheep by nuclear transfer, and for the basic analysis of transgenic organisms
 
Audience Level
0
Audience Level
1
  Kids General Special  
Audience level: 0.39 (from 0.00 for Transgenes ... to 0.39 for Transgenes ...)
Languages
English (15)
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