WorldCat Identities

Theophilus, Bimal D. M.

Works: 4 works in 31 publications in 1 language and 1,055 library holdings
Genres: Laboratory manuals 
Roles: Author, Editor
Publication Timeline
Most widely held works by Bimal D. M Theophilus
PCR mutation detection protocols by Bimal D. M Theophilus( )

27 editions published between 2002 and 2011 in English and held by 1,051 WorldCat member libraries worldwide

Since the publication of the popular first edition, the explosion of DNA sequence information, the access to bioinformatics and mutation databases coupled with the ability to readily detect and confirm mutations has cemented the role of molecular diagnostics in medicine and, in particular, mutation detection by the polymerase chain reaction (PCR). In PCR Mutation Detection Protocols, Second Edition, expert researchers bring the subject up-to-date with key protocols involving the PCR and its many various incarnations such as SSCP, CSGE, and dHPLC. The volume also addresses key areas such as Southern blotting, accurate diagnostics with high throughput, as well as microarray systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes which provide the often hard to find information that may mean the difference between the success and failure of the method. Authoritative and cutting-edge, PCR Mutation Detection Protocols, Second Edition aims to stimulate postgraduate scientists, researchers, and clinicians already engaged in the area and to provide an important first step for those new to this practice wanting to adopt the powerful and essential technique in their own laboratories
PCR Mutation Detection Protocols by Bimal D. M Theophilus( )

2 editions published in 2011 in English and held by 3 WorldCat member libraries worldwide

PCR Mutation Detection Protocols. Methods in Molecular Biology, Volume 187( )

1 edition published in 2002 in English and held by 1 WorldCat member library worldwide

Application of DNA technology to the identification of disease-causing mutations has become widespread in recent years. PCR Mutation Detection Protocols, provides biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g., ASO-PCR, SSCP, DGGE, chemical cleavage), deletions (multiplex PCR, FISH, blotting), non-sense mutations (PTT), and more. The new and exciting techniques of DNA array analysis, along with such recently developed experimental methods as conformation-sensitive gel electrophoresis, are also included. Additional coverage is given to the direct use of DNA sequencing as a detection method in its own right and to the characterization of mutations previously located by other screening techniques. Each chapter explains the basic theory behind the technique and provides valuable notes essential for its successful execution. Comprehensive and highly practical, PCR Mutation Detection Protocols assures both seasoned and novice investigators access to the highly productive and readily reproducible PCR-based mutation detection methods, techniques that are laying the groundwork for many of today's major scientific and medical advances
PCR Mutation Detection Protocols( )

1 edition published in 2011 in English and held by 0 WorldCat member libraries worldwide

Audience Level
Audience Level
  Kids General Special  
Audience level: 0.27 (from 0.27 for PCR mutati ... to 1.00 for PCR Mutati ...)

PCR mutation detection protocols
English (31)