WorldCat Identities

Salgaller, Michael L.

Overview
Works: 5 works in 11 publications in 1 language and 56 library holdings
Genres: Academic theses 
Roles: Author
Classifications: HD9999.B442, 660.60684
Publication Timeline
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Most widely held works by Michael L Salgaller
Biotechnology entrepreneurship : from science to solutions by Michael L Salgaller( Book )

3 editions published in 2010 in English and held by 48 WorldCat member libraries worldwide

Use of Expression Cloning to Identify Novel Antigens Recognized on Human Prostate Tumors to Act as Targets of Cellular Immunotherapy( Book )

4 editions published between 1999 and 2001 in English and held by 4 WorldCat member libraries worldwide

Our aim is to identify epitopes on the surface of prostate cells that are largely or exclusively tissue- or tumor- specific. This is done by expansion of T cells reactive for HLA-restricted, established tumor lines from the peripheral blood cells and tumor-infiltrating lymphocytes of cancer patients. Towards this end, we established a reagent bank of patient material that includes tissue, cells, and serum. We successfully and stably gene-modified the established prostate cell line, LNCaP, so that it expresses HLA-A2 and HLA-Al. We successfully transfected two other cell lines with these genes, transiently but sufficiently, for our in vitro stimulation technique. Several potentially HLA-restricted, tumor-specific T cells have been identified and are currently being cloned for retesting. In order to study tissue distribution, primary normal and tumor prostate tissue are being cultured by methods developed in our laboratory. In addition, we are performing an antibody-based method of antigen discovery that utilizes patient serum. Using this approach, several genes have been identified some of which represent previously unreported, novel genes. Studies are underway to discern the exact epitope being recognized. Studies are also underway to assess the level of gene expression in normal vs. tumor tissue from representative regions of the body
RAS and GLI gene activity in tumors of meningeal and glial origin by Michael L Salgaller( )

2 editions published in 1989 in English and held by 2 WorldCat member libraries worldwide

We examined the expression of ras and gli genes among histological subtypes of tumors using three parameters: (1) Immunohistochemistry with an anti-p21 monoclonal antibody, and (2), (3) in situ hybridization to determine the extent of ras and gli mRNA expression. Data was quantified via standard methods of light microscopy, as well as computer-assisted, image-analysis to obtain a quantized function of two-dimensions, generated by repeatable optical means
Biotechnology entrepreneurship - where no research has gone before by Michael L Salgaller( )

1 edition published in 2010 in English and held by 2 WorldCat member libraries worldwide

Expression of MAGE genes in ocular melanoma during progression from primary to metastatic disease by Peter W Chen( )

1 edition published in 1997 in English and held by 0 WorldCat member libraries worldwide

 
Audience Level
0
Audience Level
1
  Kids General Special  
Audience level: 0.56 (from 0.51 for Biotechnol ... to 1.00 for Use of Exp ...)

Biotechnology entrepreneurship : from science to solutions
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Languages
English (11)