WorldCat Identities

Charpentier, Emmanuelle 1968-

Overview
Works: 5 works in 11 publications in 2 languages and 65 library holdings
Genres: Laboratory manuals 
Roles: Editor, 958
Publication Timeline
.
Most widely held works by Emmanuelle Charpentier
CRISPR : methods and protocols by Magnus Lundgren( Book )

7 editions published in 2015 in English and held by 61 WorldCat member libraries worldwide

This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels. CRISPR: Methods and Protocols guides readers through techniques that have been developed specifically for the analysis of CRISPR-Cas and techniques adapted from standard protocols of DNA, RNA and protein biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, CRISPR: Methods and Protocols provides a broad list of tools and techniques to study the interdisciplinary aspects of the prokaryotic CRISPR-Cas defense systems
Extracellular Vesicle RNA: A Universal Mediator of Microbial Communication?( )

1 edition published in 2018 in English and held by 1 WorldCat member library worldwide

Abstract : Both extracellular RNAs and extracellular vesicles (EVs) have recently garnered attention as novel mediators of intercellular communication in eukaryotes and prokaryotes alike. EVs not only permit export of RNA, but also facilitate delivery and trans-kingdom exchange of these and other biomolecules, for instance between microbes and their hosts. In this Opinion article, we propose that EV-mediated export of RNA represents a universal mechanism for interkingdom and intrakingdom communication that is conserved among bacterial, archaeal, and eukaryotic microbes. We speculate how microbes might use EV RNA to influence target cell gene expression or manipulate host immune responses. Highlights: Multiple lines of evidence point to EVs as universal carriers of extracellular RNA from bacteria, archaea, fungi, and protists. Microbial EV RNAs are often enriched in small, noncoding RNAs with stable secondary structures that may influence their packaging in EVs or their function in target cells. Microbial EV RNAs can enter the cytoplasm of target cells to influence gene expression. The EV RNA cargo likely contributes to the diverse immunomodulatory activities of microbial EVs by sequence-specific silencing of target genes and by activating innate immune receptors in host cells. Microbial mRNAs are considered signals of microbial viability (vita-PAMPs), and can be transported by EVs
Etude fonctionnelle de la MMp - 12 de macrophage en vue de son ciblage thérapeutique dans la broncho-pneumopathie chronique obstructive by Anne-Sophie Lamort( )

1 edition published in 2015 in French and held by 1 WorldCat member library worldwide

Chronic obstructive pulmonary disease or COPD is a lung disease caused by tobacco smoking. This is a chronic and non reversible disease for which no curative treatment is available yet. Permanent inflammation of the airways is a hallmark of COPD because immune cells such as neutrophils and macrophages are continuously recruited. Once activated, these cells release numerous active proteases which participate to the degradation of structural proteins of the lungs such as elastin, leading to lung emphysema as a consequence of lung alveoli degradation. Among the different proteases found in the lungs, macrophage MMP-12 has been reported to play a key pathogenic role in COPD development
Functional analysis of the group A streptococcal luxS/AI-2 system in metabolism, adaptation to stress and interaction with host cells by Maria Siller( )

1 edition published in 2008 in English and held by 1 WorldCat member library worldwide

Background The luxS /AI-2 signaling pathway has been reported to interfere with important physiological and pathogenic functions in a variety of bacteria. In the present study, we investigated the functional role of the streptococcal luxS /AI-2 system in metabolism and diverse aspects of pathogenicity including the adaptation of the organism to stress conditions using two serotypes of Streptococcus pyogenes , M1 and M19. Results Exposing wild-type and isogenic luxS -deficient strains to sulfur-limited media suggested a limited role for luxS in streptococcal activated methyl cycle metabolism. Interestingly, loss of luxS led to an increased acid tolerance in both serotypes. Accordingly, luxS expression and AI-2 production were reduced at lower pH, thus linking the luxS /AI-2 system to stress adaptation in S. pyogenes . luxS expression and AI-2 production also decreased when cells were grown in RPMI medium supplemented with 10% serum, considered to be a host environment-mimicking medium. Furthermore, interaction analysis with epithelial cells and macrophages showed a clear advantage of the luxS -deficient mutants to be internalized and survive intracellularly in the host cells compared to the wild-type parents. In addition, our data revealed that luxS influences the expression of two virulence-associated factors, the fasX regulatory RNA and the virulence gene sibA ( psp ). Conclusion Here, we suggest that the group A streptococcal luxS /AI-2 system is not only involved in the regulation of virulence factor expression but in addition low level of luxS expression seems to provide an advantage for bacterial survival in conditions that can be encountered during infections
RNase Y-mediated regulation of the streptococcal pyrogenic exotoxin B by L Broglia( )

1 edition published in 2018 in English and held by 1 WorldCat member library worldwide

Endoribonuclease Y (RNase Y) is a crucial regulator of virulence in Gram-positive bacteria. In the human pathogen  Streptococcus pyogenes , RNase Y is required for the expression of the major secreted virulence factor streptococcal pyrogenic exotoxin B (SpeB), but the mechanism involved in this regulation remains elusive. Here, we demonstrate that the 5′ untranslated region of  speB  mRNA is processed by several RNases including RNase Y. In particular, we identify two RNase Y cleavage sites located downstream of a guanosine (G) residue. To assess whether this nucleotide is required for RNase Y activity  in vivo , we mutated it and demonstrate that the presence of this G residue is essential for the processing of the  speB  mRNA 5′ UTR by RNase Y. Although RNase Y directly targets and processes  speB , we show that RNase Y-mediated regulation of  speB  expression occurs primarily at the transcriptional level and independently of the processing in the  speB  mRNA 5′ UTR. To conclude, we demonstrate for the first time that RNase Y processing of an mRNA target requires the presence of a G. We also provide new insights on the  speB  5′ UTR and on the role of RNase Y in  speB  regulation
 
Audience Level
0
Audience Level
1
  Kids General Special  
Audience level: 0.65 (from 0.63 for CRISPR : m ... to 0.97 for RNase Y-me ...)

Alternative Names
Charpentier, Emmanuelle Marie 1968-

Emmanuelle Charpentier Frans microbiologe

Emmanuelle Charpentier fransk forskare inom mikrobiologi och immunologi

Emmanuelle Charpentier französische Mikrobiologin und Immunbiologin

Emmanuelle Charpentier French microbiologist and biochemist

Emmanuelle Charpentier microbiòloga i bioquímica francesa

Emmanuelle Charpentier microbióloga y bioquímica frabcesa

Emmanuelle Charpentier microbióloga y bioquímica francesa

Emmanuelle Charpentier microbiologiste, généticienne et biochimiste française

Шарпентье, Эммануэль

עמנואל שרפנטייה

امانوئل چارپتیپر

エマニュエル・シャルパンティエ

伊曼紐•夏彭提耶

艾曼紐·卡彭特

Languages
English (10)

French (1)