WorldCat Identities

Fineran, Peter C.

Works: 3 works in 11 publications in 1 language and 156 library holdings
Genres: Laboratory manuals 
Roles: Editor
Publication Timeline
Most widely held works by Peter C Fineran
CRISPR : methods and protocols by Magnus Lundgren( )

9 editions published in 2015 in English and held by 154 WorldCat member libraries worldwide

This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels. CRISPR: Methods and Protocols guides readers through techniques that have been developed specifically for the analysis of CRISPR-Cas and techniques adapted from standard protocols of DNA, RNA and protein biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, CRISPR: Methods and Protocols provides a broad list of tools and techniques to study the interdisciplinary aspects of the prokaryotic CRISPR-Cas defense systems
Regulation of CRISPR-Cas adaptive immune systems( )

1 edition published in 2017 in English and held by 1 WorldCat member library worldwide

Highlights: CRISPR-Cas systems provide adaptive defense against invading genetic elements. Controlling CRISPR-Cas activity enables optimization of the immune response. CRISPR-Cas regulation involves an integrated network that acts at multiple levels. Regulatory strategies can be conserved between different systems and strains. Diverse regulatory mechanisms exist that likely reflect varied selective pressures. Abstract : CRISPR-Cas systems are prokaryotic immune systems that allow defense against invasion by foreign genetic elements. Through the acquisition of genetic memory representing prior exposure, the CRISPR-Cas machinery identifies complementary nucleic acids and mediates their destruction. Since their discovery, CRISPR-Cas research has exploded, with major progress into their mechanism and biotechnological application. In contrast, we are only beginning to understand how CRISPR-Cas activity is regulated. Recent studies have uncovered both conserved regulatory pathways, in addition to more specific mechanisms. Identifying the stimuli linking invasion with CRISPR-Cas activation will be essential in unraveling their ecological roles, evolutionary success and might offer strategies to control immunity for particular applications. We highlight factors impinging on CRISPR-Cas regulation across diverse bacterial and archaeal species
AbiEi Binds Cooperatively to the Type IV abiE Toxin–Antitoxin Operator Via a Positively-Charged Surface and Causes DNA Bending and Negative Autoregulation( )

1 edition published in 2018 in English and held by 1 WorldCat member library worldwide

Abstract: Bacteria resist phage infection using multiple strategies, including CRISPR-Cas and abortive infection (Abi) systems. Abi systems provide population-level protection from phage predation, via "altruistic" cell suicide. It has recently been shown that some Abi systems function via a toxin–antitoxin mechanism, such as the widespread AbiE family . The Streptococcus agalactiae AbiE system consists of a bicistronic operon encoding the AbiEi antitoxin and AbiEii toxin, which function as a Type IV toxin–antitoxin system. Here we examine the AbiEi antitoxin, which belongs to a large family of transcriptional regulators with a conserved N-terminal winged helix-turn-helix domain. This winged helix-turn-helix is essential for transcriptional repression of the abiE operon. The function of the AbiEi C-terminal domain is poorly characterized, but it contributes to transcriptional repression and is sufficient for toxin neutralization. We demonstrate that a conserved charged surface on one face of the C-terminal domain assists sequence-specific DNA binding and negative autoregulation, without influencing antitoxicity. Furthermore, AbiEi binds cooperatively to two inverted repeats within the abiE promoter and bends the DNA by 72°. These findings demonstrate that the mechanism of DNA binding by the widespread family of AbiEi antitoxins and transcriptional regulators can contribute to negative autoregulation. Graphical Abstract: Highlights: The Type IV antitoxin AbiEi has a positive surface charge that aids DNA binding. AbiEi binds cooperatively to two inverted repeats overlapping the abiE promoter. The AbiEi C-terminal domain binds to the extended region of the inverted repeats. AbiEi binding causes DNA bending and repression of the abiE toxin–antitoxin operon
Audience Level
Audience Level
  Kids General Special  
Audience level: 0.63 (from 0.63 for CRISPR : m ... to 0.88 for AbiEi Bind ...)

English (11)