WorldCat Identities

Washington State University Department of Chemistry

Overview
Works: 145 works in 146 publications in 1 language and 609 library holdings
Genres: Academic theses 
Publication Timeline
.
Most widely held works about Washington State University
 
Most widely held works by Washington State University
Understanding and utilizing DOTA coordination chemistry for trivalent lanthanide/actinide separation by Jessica Ann Drader( )

1 edition published in 2012 in English and held by 5 WorldCat member libraries worldwide

Utilizing the understanding gained from a lanthanide series studies, a series of kinetic studies for a solvent extraction system combining the cation exchanging extractant Cyanex 272 with DOTA using radiotracer 152,154Eu3+, 147Nd3+, and 241Am3+ to monitor the rate of progress of the phase transfer reaction were completed. These results indicate that DOTA prefers to form complexes with the actinides over the lanthanides, confirming the theoretical prediction. Finally, reported in this work is a study done to determine the reversibility of the AmDOTA-complex and the ability to break the DOTA "cage". It was observed that at pH 1.0, 60°C, it will take between 15 hours and 6 days for the complex to fully dissociate. Suggestions on improving this condition are provided
Electrochemical investigations of dopamine using rotating disk electrode voltammetry : Study of release and reuptake kinetics and inhibition of the neuronal dopamine transporter by Peter Alan Lukus( )

1 edition published in 2013 in English and held by 5 WorldCat member libraries worldwide

Chapter Four presents analysis of DAT function in hooded rats afflicted with Parkinson's symptoms. Transport rates of exogenous DA uptake, as well as stimulated DA release and subsequent reuptake, are measured. DAT kinetic function is shown to be retained in Parkinson's rats, suggesting that the transporter is unaffected by the disease in the striatal tissue
Application of hadamard transform ion mobility mass spectrometry to global metabolomics by Xing Zhang( )

1 edition published in 2014 in English and held by 5 WorldCat member libraries worldwide

Conventional Ion mobility mass spectrometry (IMMS) provides rapid separation and detection of complex mixtures. It is merging as a powerful analytical platform for the field of metabolomics but is limited in throughput. Global metabolomics aims at comprehensive measurement for all metabolites and presents challenges in analytical technique. The work describe herein evaluates the capability of hadamard transform ion mobility mass spectrometry (HT-IMMS) for comprehensive metabolomics analysis with high throughput and high resolving power. The work also presents a number of applications of global metabolomics regarding to biological systems including human blood, rat brain tissue and mice plasma
New technologies : Protein interactions and post translational modifications by Li Yang( )

1 edition published in 2011 in English and held by 5 WorldCat member libraries worldwide

Using Electrospray Ionization, Mass Spectrometry to observe metal, complexant species relevant to the nuclear fuel cycle by Luther Wilbur McDonald( )

1 edition published in 2013 in English and held by 5 WorldCat member libraries worldwide

ESI-MS is routinely used qualitatively for determining the stoichiometry of metal-ligand complexes, but deviations from known solution speciation generated by the ESI-MS have prevented this technique from providing consistent and reliable quantitative data. Fundamental studies were performed to identify discrepancies between solution and gas phase speciation. Redox reactions and differing ionization efficiencies between the metal and complexant were primary factors causing alterations to solution speciation in the ESI source. Furthermore, the produced gas phase ions were altered by fragmentation regions within the MS. Establishing the fundamentals of metal-complexant speciation in ESI-MS will define the quantitative limits of this technique
Synthetic Uses of S-Nitrosothiols in Organic Chemistry by Tyler David Biggs( )

1 edition published in 2017 in English and held by 5 WorldCat member libraries worldwide

S-Nitrosothiols have risen to prominence since their identification as an important post-translational modification of cysteine residues. The full range of chemistry and biological implications of this motif are under exploration. Direct chemical reactions of S-nitrosothiols should reveal clues into their biological effects, and produce new synthetic tools for the construction of sulfur and nitrogen containing compounds
Enzymatic degradation of polychlorophenols by Robert P Hayes( )

1 edition published in 2013 in English and held by 5 WorldCat member libraries worldwide

This dissertation is composed of three studies that investigate enzymes, one from each organism mentioned above, that conduct unique chemistry and thus were selected for structure determination, biochemical characterization and mechanistic analysis. The first study was a structural and catalytic comparison between 2,4,6-trichlorophenol-4-monooxygenase (TcpA) from C. necator JMP134 and 2,4,5-trichlorophenol-4-monooxygenase (TftD) from B. phenoliruptrix AC1100. We determined the crystal structure of TcpA and conducted docking experiments that suggest Alanine 293 in TcpA and Isoleucine 292 in TftD are the direct cause for differences in substrate specificity between the two enzymes. We also propose a mechanism for hydrolytic dechlorination in TcpA. The second study investigated a non-heme Fe (II) dioxygenase, 2,6-dichloro- p-hydroquinone 1,2-dioxygenase (PcpA), from S. chlorophenolicum. The crystal structure, catalytic residues and enzyme kinetics for PcpA were determined. We discovered the substrate p-hydroxyl group was essential for binding and thus phenols and catechols did not bind to PcpA, distancing it from other known intradiol and extradiol catechol dioxygenases. We propose a general reaction mechanism for the novel p-hydroquinone 1,2-dioxygenases. The third study characterized 5-chlorohydroxyhydroquinone dehydrochlorinase (TftG) from B. phenoliruptrix AC1100. We determined the crystal structure of TftG in both apo-form and complex-form with the product analog 2,5-dihydroxybenzoquinone. Site-directed mutagenesis was conducted to confirm the role of several active site residues. Our data support the presence of a histidine-aspartic acid catalytic dyad and an oxyanion hole in the active site. The residues implicated for catalysis in TftG were conserved across the YCII-superfamily proteins of largely unknown function. We determined the signature sequence for the YCII-superfamily proteins and propose they are likely a family of aromatic ring lyases
Assessing isomeric heterogeneity of carbohydrates by ion mobility mass spectrometry (IMMS) by Hongli Li( )

1 edition published in 2013 in English and held by 5 WorldCat member libraries worldwide

Ion mobility spectrometry (IMS) coupled to different types of mass spectrometry (MS), which create a powerful analytical tool called ion mobility mass spectrometry (IMMS), were employed to evaluate the isomeric heterogeneity of carbohydrates. Isomer separation was achieved on millisecond timescale for a wide variety of carbohydrate compounds including monosaccharide methyl glycosides, disaccharides, oligosaccharide-alditols isolated from glycoprotein and glycopeptides from glycoprotein digests using drift tube IMS or/and traveling wave IMS coupled to time of flight MS. With IMS coupled to tandem MS, fragmentation patterns for the individual resolved isomeric mobility peaks can be obtained, providing evidence for multiple isomeric precursors as mobility-separated species. The special design of Synapt G2 high definition MS, where a collision dissociation cell is located in front of and after traveling wave IMS, respectively, enables both mobility-resolved precursor ions to be fragmented and mobility-separated product ions to be dissociated. Many isomeric product ions were observed for parent disaccharides and higher oligosaccharide-alditols. And mobility-resolved product ions from mass-selected oligosaccharide-alditol precursors yielded different characteristic mass spectra. A hybrid tandem IMMS instrument, IMMS-IMMS, dual gate drift tube IMS coupled to Synapt G2 MS was established. In addition to its capability in evaluating the structural complexity of precursor ions, ion mobility identity of product ions derived from individual mobility- and mass-selected precursor ions from a mixture of isomers were able to be measured for the first time. This provides a valuable tool to establish a direct precursor-product relationship between mobility-selected precursor ions and specific sets of product ions having unique mobilities. N-linked glycoprotein digests, without deglycosylation, were subjected to IMMS analysis directly. Mixtures of peptides and glycosylated peptides with different charge states were separated on different ion mobility-mass correlation trend lines. Tedious and time-consuming sample preparation was eliminated and the detection of low abundance ions was improved
Development of novel controllable hydrogen sulfide donors by Yu Zhao( )

1 edition published in 2014 in English and held by 5 WorldCat member libraries worldwide

To this end, we have developed new controllable H2S donors, such as N-mercapto-based donors and perthiol-based donors. These molecules were stable in aqueous solutions. However, in the presence of cellular thiols (i.e. cysteine and glutathione), time-dependent H2S release was observed. In addition, protective effects of these donors in living systems were also observed. These findings suggest that both N-mercapto-based and perthiol-based molecules are potent H2S donors and they have potential therapeutic benefits due to H2S release
Separation and identification of metal ions via ligand assisted capillary electrophoresis by Jane Holly House( )

1 edition published in 2013 in English and held by 5 WorldCat member libraries worldwide

Capillary electrophoresis (CE) is a powerful tool for analytical separations. These separations are achieved based on analyte charge and size, allowing for the simultaneous separation and detection of positive, negative and neutrally charged species. The addition of complexing ligands to the background electrolyte solution further enhances separation efficiency. In this work, the separation of the group II metal cations including Be(II) and its hydrolysis products has been demonstrated. It was observed that surface geometry plays a role in elution order for these hydrolytic species. The hydrolysis of aluminum in the presence of hydroxycarboxylic acids was also investigated using CE and mass spectrometry. These experiments confirmed earlier observations of Al-27 NMR work. Finally, the separation of Al from Ga and other Group 13 metals and Fe(III) was pursued utilizing strongly complexing ligands
Development of a breathalyzer : Ion mobility spectrometer for the detection of cannabis from human breath by Jessica Ann Tufariello( )

1 edition published in 2016 in English and held by 5 WorldCat member libraries worldwide

Twenty-three states have legalized cannabis for medicinal uses and four states plus the District of Columbia legalized cannabis for recreational use. It is expected cannabis laws will continue to change, which increases the number of impaired drivers on the road. Currently, the only line of defense against drugged drivers is a drug recognition expert, but as of 2013, there were only 230 employed in Washington State to regulate the 5 million licensed drivers. Unlike the use of alcohol breathalyzers by law enforcement officers for the rapid identification of alcohol-impaired drivers, there are no roadside chemical tests for screening and identifying drugged drivers. The development of a roadside test for the presence of drugs will aid law enforcement officers in making rapid and informed decisions about impaired drivers
Effect of iron-nitrogen coordination (type II binding) on drug metabolism by cytochrome p450 : Studies to understand the kinetics, metabolic stability and regioselectivity by Upendra Purush Dahal( )

1 edition published in 2012 in English and held by 5 WorldCat member libraries worldwide

This work is focused to understand the metabolism, metabolic stability and regioselectivity of type II binding compounds. To explore the metabolism of type II binding compounds, we used saturation kinetics, surface plasmon resonance and electrochemical studies. Three possible mechanisms were described and analyzed to identify the kinetic of metabolism. Furthermore, to determine the effect of the type II binding on the metabolic stability of the drugs we developed a small library of the quinoline carboxamide compounds to systematically compare the metabolic stability and regioselectivity between structurally similar type I and type II binding compounds. The library contained same core structure but number and position of the nitrogen ware varied. On the basis of kinetic parameters variation in metabolic stability and regioselectivity was explained for the two binding modes
Functionalization characterization and evaluation of dipicolinate derivatives with f-element complexes by Colt Richard Heathman( )

1 edition published in 2013 in English and held by 5 WorldCat member libraries worldwide

Fissile materials like 235U and 239Pu are the most energy dense fuel source known to mankind. The attraction to harnessing atomic energy in nuclear power plants has been gaining attention as an alternative clean and efficient power source. A limiting factor for utilizing this technology as a fuel source stems from the political, financial, and technological limitations related to reprocessing of spent nuclear fuel. To consider nuclear power as a primary means of electricity in the future, closing the nuclear fuel cycle and managing the waste generated from these processes is essential. Closing the nuclear fuel cycle through reprocessing spent nuclear fuel and transmutation of the long lived decay product actinides has the potential to enhance nuclear energy sustainability and minimize radiotoxicity hazards of irradiated fuels. Currently, the only method of industrial-scale reprocessing in the world is the PUREX process, for the recovery of uranium and plutonium. Separation and transmutation of the long lived minor actinides is crucial for reducing the time spent nuclear material is to be stored in a geological repository. Several processes in developmental stages (SANEX, DIAMEX, TRUSPEAK, and TALSPEAK), are being evaluated for the partitioning of fission product lanthanides from actinides. The separation of lanthanides from transplutonium actinides is a key step for advancing a closed nuclear fuel cycle. In the U.S., TALSPEAK-like processes are being evaluated for this task. Partitioning of the concentrated lactic acid buffer in the TALSPEAK process complicates this already complex chemistry. To reduce or eliminate the undesirable effect of lactic acid, an alternative aqueous feed is to be evaluated. The use of dipicolinic acid (DPA) as the actinide selective stripping agent could bring significant simplification. Overcoming solubility limitations of DPA in acidic media was evaluated through functionalization of the 4-position on the pyridine ring. Structural affects on the coordination of f-elements were evaluated using a combination of spectroscopy and solvent extraction techniques. The findings of these studies and corresponding implications to nuclear fuel reprocessing will be discussed
Pressure-induced physical and chemical changes of non-conventional energetic materials : Nitrate, perchlorate and peroxide chemistries at high pressures and high temperatures by Mihindra Dunuwille( )

1 edition published in 2015 in English and held by 5 WorldCat member libraries worldwide

Non-conventional explosives are widely used in terrorist activities, thus imposing a threat to homeland security. The simple combination of oxidizers with fuels is one popular category. Our goal is to explore and understand chemistry in selected oxidizers over a relevant pressure-temperature (P-T) regime of blast conditions, to get insights into fundamental chemistry associated with the selected energetic molecules and energetic processes at molecular level, for mitigation applications. We have investigated ammonium nitrate (AN), ammonium perchlorate (AP), and simple group IA peroxides under high-pressure high-temperature conditions, using confocal micro-Raman spectroscopy and synchrotron x-ray diffraction
Biological Peptide Interactions with Cellular Membranes by Brandan Michael Cook( )

1 edition published in 2017 in English and held by 5 WorldCat member libraries worldwide

The peptide-membrane interaction studies mentioned above opened the research doors to a Prostate cancer (PCa) peptide-transmembrane domain (TMD) inhibition study. PCa is the second leading cause of cancer-related deaths among American males. The high expression of a membrane protein called Prostate Specific Membrane Antigen (PSMA) is implicated in PCa invasiveness as well as neovasculature of nonprostatic solid tumors. Further, it is widely recognized that many membrane proteins participate in intracellular signal transduction through the lateral association of their TMDs, but this has not been directly demonstrated in PSMA. Thus, we aimed to determine if the TMD could be perturbed by engineered synthetic peptides
Activation product analysis in the presence of fission products by Samuel Spencer Morrison( )

1 edition published in 2015 in English and held by 5 WorldCat member libraries worldwide

Methods to quantify activation products in complex samples are needed in areas such as nuclear safeguards, waste treatment and identification, environmental monitoring, and consequence management. Formation of activation products is often concurrent with the formation of large quantities of fission products that intermix with the activation products. As many fission products have high rates of gamma emissions, the presence of fission products obfuscate the gamma spectrum and increase the minimum detectable activity (MDA) limit for activation products. To achieve the lowest detection limits possible for activation product analysis of 55Fe, 182Ta, 187W, 197Pt, and 198Au, radiochemical separation procedures were developed and evaluated on multiple sample types including samples containing fission products
New methods for the convergent glycoligation of peptides by Ryan Joseph( )

1 edition published in 2015 in English and held by 5 WorldCat member libraries worldwide

The attachment of oligosaccharides (glycans) to proteins is an important co- and post-translational modification which greatly expands the functional and structural diversity of a proteome. As glycosylation is integral to many biological processes, studies in this area provide valuable and far reaching insights. Herein is described the chemoselective Cu(II)-HOBt promoted chemical ligation of glycosylamines and peptide thioacids to give N-glycosylated peptides. The method is distinguished from other chemical approaches to peptide N-glycosylation in that: (1) it can be employed in the presence of unprotected N-terminal and Lys sidechain amines, (2) it is remarkably fast, going to completion in under 30 minutes, and (3) it produces glycopeptide without attendant aspartimide formation. The N-glycoligation is exemplified by the coupling of chitobiosylamine and an unprotected heptapeptide containing an aspartyl thioacid side-chain to produce an N-linked glycopeptide corresponding to a truncated segment of a human glycoprotein hormone. The N-glycoligation provides a simple and convergent means for attaching a carbohydrate to a specific Asp residue in an unprotected peptide
Discovering biochemical signatures of disease : Metabolomics of colorectal cancer via ion mobility mass spectrometry by Michael David Williams( )

1 edition published in 2014 in English and held by 5 WorldCat member libraries worldwide

Colorectal cancer (CRC) is widely recognized as one of the leading causes of cancer related mortality worldwide. This, despite the fact that CRC is a completely curable disease, when caught early enough. There is a high rate of mortality for this disease due to the delayed detection and diagnosis of disease presence. Early identification of polyps that may progress to overt CRC is the cornerstone of CRC prevention
Optimization of the electrochemical pre-concentration of f-elements by Jamie L Doyle( )

1 edition published in 2014 in English and held by 5 WorldCat member libraries worldwide

Rapid pre-concentration of trace lanthanide cations in aqueous solution has been achieved using mercury film electrodes. Although effective, this technique has shown minimal sample carryover when used to pre-concentrate trace level plutonium. With potential applications to nuclear forensics and environmental monitoring, maximum sample pre-concentration is critical for accurate analysis. Through work investigating the characteristics of various aqueous matrices, optimal working conditions can be found and applied to a range of f-elements. Additional work in understanding the mechanism of metal adsorption to the mercury film electrode contributes to optimization of the technique. To date, the influence of the mercury film has shown to be significant in the pre-concentration process, most likely due to the adsorption being a function of metal amalgamation
Measuring membrane mobility : a comparison of three techniques including instrument and method development by Adam O'Neill Barden( )

1 edition published in 2014 in English and held by 5 WorldCat member libraries worldwide

A major component of this dissertation was the design and construction of three different optical systems. Measurement of membrane mobility was studied using all three techniques. The three microscopes were a single molecule microscope in total internal reflectance mode for single particle tracking, a confocal fluorescence correlation spectroscopy microscope, and a fluorescence recovery after photobleaching microscope. Additionally, a thermal control system was designed and built to regulate the temperature of both the sample and objective for all three microscopes with a thermal accuracy of +/-0.1 & deg;C. The system was designed so that both the sample and the objective could be either heated or cooled to promote system stability and had a useful range of -10 -- 47 & deg;C for these experiments
 
moreShow More Titles
fewerShow Fewer Titles
Audience Level
0
Audience Level
1
  Kids General Special  
Audience level: 0.41 (from 0.40 for Biological ... to 0.76 for Chemistry, ...)

Alternative Names

controlled identityWashington State University

Washington State University. Dept. of Chemistry

Languages
English (24)