WorldCat Identities

Henry, Raymond L.

Overview
Works: 25 works in 48 publications in 1 language and 363 library holdings
Roles: Author
Publication Timeline
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Most widely held works by Raymond L Henry
Sickle cell; a scope publication [by] Marion I. Barnhart, Raymond L. Henry [and] Jeanne M. Lusher by Marion I Barnhart( Book )

10 editions published between 1974 and 1976 in English and held by 256 WorldCat member libraries worldwide

Sickle cell by Marion I Barnhart( Book )

9 editions published between 1974 and 1979 in English and held by 88 WorldCat member libraries worldwide

An Automated Screening Method for the Specific Detection of Homozygous and Heterozygous S Hemoglobin by Raymond L Henry( Book )

3 editions published in 1970 in English and held by 2 WorldCat member libraries worldwide

The Murayama test although a specific test for the molecular lesion of S hemoglobin, is not convenient for use in the detection of S hemoglobin in large human populations. The principles of that modification have been adapted to the automated technique described in this report. When S hemoglobin is introduced into the Sickledex working solution the S hemoglobin 'sickles, ' constituting a nematic liquid crystal system. In that physical state of hemoglobin, this system acquires several remarkable properties including a marked gain in turbidity. From previous studies it was known that urea will selectively attack specified hydrophobic bonds necessary to the sickling event in S hemoglobin. Accordingly, it was found that when a specimen of S hemoglobin is divided and traversed in phase along two channels, a comparison of the percent transmittance of the two aliquots will show that the specimen in the urea-Sickledex line gains in percent transmittance as a quantitative function of the amount of S hemoglobin present. The method is sensitive enough to detect both homozygous and heterozygous S hemoglobin. An inexpensive, automated technique for the specific detection of S hemoglobin suitable for the mass survey of large human populations has been developed and is now available
Sickle cell( Visual )

1 edition published in 1975 in English and held by 2 WorldCat member libraries worldwide

Self-instructional unit presents scanning electron microscope comparisons of normal and sickled red blood cells. Presents photographs and radiographs of sickle cell anemia manifestations (dactylitis, bone changes, leg ulcers)
Sickledex Test for S Hemoglobin: A Critique by Robert M Nalbandian( Book )

1 edition published in 1971 in English and held by 1 WorldCat member library worldwide

When the reagents, reactions, and principles of the Sickledex test are understood in terms of modern molecular biology, it is believed that the Sickledex test can be an invaluable and productive tool in the discovery not only of hemoglobin S but other hemoglobinopathies as well, provided: (1) certain pitfalls of false positive and false negative reactions are recognized; (2) urea is used to modify the test; and (3) all positive Sickledex specimens are subsequently studied by hemoglobin electrophoresis technique. (Author)
A progress report on the mineral analysis of deep well water conditions in Dallas county by Raymond L Henry( )

1 edition published in 1949 in English and held by 1 WorldCat member library worldwide

Modified Sickledex Tube Test: A Specific Test for 5 Hemoglobin( Book )

1 edition published in 1970 in English and held by 1 WorldCat member library worldwide

The Sickledex test is a screening method for the detection of S hemoglobin. On the basis of analytical studies conducted, the Sickledex test is a version of the Itano solubility test which is predicted on the unique and extraordinary insolubility of deoxygenated hemoglobin S in phosphate buffer systems. It was shown that other non-S sickling hemoglobins and also unstable hemoglobins may and can give false positive Sickledex tests. The Sickledex test was modified by the reversal of a positive test in the presence of urea when the specimen is S hemoglobin. If the test specimen is a non-S sickling hemoglobin the positive test will remain positive in the presence of urea. This differential property of the clearance of turbidity (the disbursal of the neumatic liquid crystal system of sickled S hemoglobin) with urea under the conditions of this test is shown to occur only in the presence of unique, specific hydrophobic bonds between interacting tetramers, a condition which Murayama has shown to be essential to the sickling event in S hemoglobin. The modified Sickledex tube test as herein described will thus identify specifically not only S hemoglobin but also non-S sickling hemoglobins as a second category of hemoglobinopathies
The Murayama Test. Part I. Evidence for the Modified Murayama Hypothesis for the Molecular Mechanism of Sickling( Book )

1 edition published in 1970 in English and held by 1 WorldCat member library worldwide

An analytical review of the literature yields considerable support for the recently modified Murayama hypothesis for the molecular mechanism of sickling in S hemoglobin. The Murayama concept implicates as essential to the sickling event in S hemoglobin specified hydrophobic bonds between interacting tetramers. Hydrophobic bonds have recently been recognized to be predominantly responsible for the steric configuration of proteins in aqueous systems. Furthermore, it is shown that the helical stability of proteins in aqueous systems is the result of counter-poised actions of hydrogen and hydrophobic bond strengths as a function of temperature. Application of this basic, general concept is made in the special case of the Murayama Test. Also, the molecular configuration of the S hemoglobin tetramer and its critical relationship to the formation of hydrophobic bonds between interacting tetramers is noted. The Murayama Test is shown to discriminate specifically for S hemoglobin on the basis of molecular structure and is presented as the first test for the detection of the specific molecular lesion of S hemoglobin. The details of the principle, technique, and experimental data of the Murayama Test are reported in Part II. (Author)
A Practical Synopsis of Consumption Coagulopathy( Book )

1 edition published in 1970 in English and held by 1 WorldCat member library worldwide

The objective of this report is to develop a practical synopsis of consumption coagulopathy. Current methodology to recognize and diagnose consumption coagulopathy is reviewed. A consumption coagulopathy panel is devised as a data source for improved methodology. A panel of very simple coagulation tests was devised which can be readily repeated, day or night, weekday or weekend, on demand. It is designated 'consumption coagulopathy panel' to serve as a source of data for diagnosis, surveillance, and therapeutic decisions con erning the case at hand. The report provides a table listing the parameters of the standard consumption coagulopathy panel and the qualitative changes which occur with each parameter for all three types. In addition, the recommended treatment is correlated with the type of consumption coagulopathy. (Author)
The Murayama test for hemoglobin S : a simplification in technique by Bruce M Nichols( Book )

1 edition published in 1971 in English and held by 1 WorldCat member library worldwide

An improvement is reported in the technique of the Murayama test, a simple specific test for hemoglobin S reported originally by this laboratory in 1970. The improvements consist of: (1) the optional use of N2 or dry ice; and (2) the elimination of several steps in the test for the preparation of concentrated hemolysates by use of a dense sucrose-detergent lysing solution. This reduces the performance time per test from 1 hour to 20 minutes, without adverse effect on reliability or accuracy of the test result. (Author)
Sickle Cell Crisis Terminated by Use of Urea in Invert Sugar in Two Cases by Robert M Nalbandian( Book )

2 editions published in 1970 in English and held by 1 WorldCat member library worldwide

The use of urea in invert sugar (UIS) for the treatment of SS crisis is related to the existence of pathologic hydrophobic bonds in sickled hemoglobin. Murayama's recently modified hypothesis for the molecular mechanism of sickling in SS hemoglobin clearly implicates the formation of hydrophobic bonds between interacting tetramers of S hemoglobin as the necessary condition for the sickling event. Hydrophobic bonds are now recognized by some biochemists to be responsible for the integrity of the steric configurations of protein molecules in aqueous systems. Urea, long known to attack hydrogen bonds and more recently recognized to attack hydrophobic bonds, has been used successfully by us as a chemical agent both to reverse and to block sickling in susceptible cells as shown elsewhere in optical and electron microscopy studies. Urea in invert sugar (UIS) has been established by a large body of literature and by Javid as a safe pharmaceutical agent; he has personally used this preparation in the treatment of over 2,000 cases of intracerebral edema. For these reasons, there was little hesitancy in using infusions of UIS in the treatment of our first two cases of SS crisis, a therapeutic strategy which has proved to be effective in these preliminary studies. Clinical histories and data are given. Precautions and principles concerning the use of UIS are discussed. A protocol including the use of controls for the evaluation of this therapeutic modality in the treatment of painful sickle cell crisis is recorded. (Author)
Consumption Coagulopathy. Practical Principles of Diagnosis and Management( Book )

1 edition published in 1970 in English and held by 1 WorldCat member library worldwide

Consumption Coagulopathy (CC), produced by multiple etiologies, is a common mechanism of death. CC, properly diagnosed and managed, often results in the extension of life over several days, yielding sufficient time for the diagnosis and treatment of the underlying disorder. With the correction of the primary disease, the threat of CC will spontaneously remit. Determination of the type of CC is critical since it may be lethal if the wrong treatment--according to type--is used. A short panel of simple tests periodically repeated and suitable for hospital laboratories will produce patterns of differential diagnostic and therapeutic significance. In CC, the central principle of pathophysiology is the intravascular conversion of plasma to serum or mimetic 'serum.' The central principle of therapy is the intravascular reversion of serum to plasma by the titration of the patient with continuous intravenous administration of the indicated drug, dosages being determined by the response of individual coagulation parameters in a periodically repeated standard panel. The physician, and especially the pathologist, because of his pivotal role in the diagnosis and management, must understand these concepts thoroughly. A practical operational approach is proposed to achieve these ends. (Author)
Embryonic, Fetal, and Neonatal Hemoglobin Synthesis: Relationship to Abortion and Thalassemia( Book )

1 edition published in 1970 in English and held by 1 WorldCat member library worldwide

In the embryo and fetus, the events and genetic mechanisms regulating the production of the five different globin chains account for several molecular species of intrauterine and neonatal hemoglobins. Strikingly intricate, synchronized correlations with reference to onset, peak level, and disappearance of: (1) five distinct globin chains; (2) five molecular species of hemoglobin; (3) three distinct intrauterine erythropoietic cell lines; ad (4) erythropoiesis in three specific embryonic and fetal organs are noted and result in new insights and findings. It is shown on the basis of correlated published data in the literature that under normal conditions of embryonic and fetal hemoglobin synthesis: (1) the erythroblasts in the yolk sac in the first trimester synthesize alpha and epsilon globin chains and so produce hemoglobins Gower-1 and Gower-2; (2) the erythroblasts in the liver in the second trimester synthesize alpha and gamma globin chains predominantly and so produce hemoglobin F; and (3) the erythroblasts in the bone marrow in the second trimester initially and in the third trimester more intensely synthesize alpha, beta, and delta globin chains and so produce hemoglobins A and A2 predominantly. Important clinical insights are gained by an understanding of these genetic, biochemical, and embryologic correlations of intrauterine erythropoiesis, including the basis: (1) for the well-known but presently unexplained peak incidence of abortions at the end of the first trimester; (2) for the delayed postnatal morphologic expression of S hemoglobin; (3) for the appearance of hemoglobinopathies Bart's and H; and (4) for the thalassemia syndromes. (Author)
Dual Automated Method for Sequential Detection of Anemia and/or Hemoglobin S by Robert M Nalbandian( Book )

2 editions published in 1973 in English and held by 1 WorldCat member library worldwide

The problem of screening large civilian and military populations for sickle cell hemoglobin has been recently approached by three distinct methods. An evaluation is attempted to determine the optimal technique. The first method to be discussed requires hemoglobin electrophoresis as the initial screening technique, followed by a solubility (dithionite) test when an 'S band, ' a nonspecific finding result, is noted. The second method requires the use of the automated dithionite test, followed, when positive, by hemoglobin electrophoresis. Several advantages are derived from the use of this sequence of tests. Also, the test has a molecular basis for specificity and the benefits of automation recommend this system for general use, especially in military populations. The third method is a dual automated method for detecting anemia and/or hemoglobin S. The initial mass screening technique involves the use of the automated dithionite test and an automated hemoglobin determination. Any specimens found to be positive by the dithionite test are studied additionally by hemoglobin electrophoresis. When such a broad spectrum of diagnostic techniques is school-centered, in effect, entire civilian populations may be screened both for hemoglobin S and anemias at remarkably low cost. (Modified author abstract)
Sickle cell disease : clinical advances by the Murayama molecular hypothesis by Army Medical Research Laboratory (U.S.)( Book )

1 edition published in 1972 in English and held by 1 WorldCat member library worldwide

A comprehensive review of the scope and thrust of clinical gains, both diagnostic and therapeutic, made in sickle cell disease as a result of analytical, deductive, and inferential extensions of the modified Murayama hypothesis for the molecular mechanism of sickling is presented. (Author)
The Murayama Test. Part II. Principles, Technique, Interpretation, and Data( Book )

1 edition published in 1970 in English and held by 1 WorldCat member library worldwide

The Murayama Test is based on the molecular mechanism of sickling for S hemoglobin as proposed and recently modified by Murayama. The principle of the test is predicated on a feature of molecular structure: hydrophobic bonds formed between interacting tetramers by the substituted no. 6 Valine near the N-terminal end of each beta-S globin chain, which are essential to the sickling event in S hemoglobin. The existence of these particular hydrophobic bonds can be visually signaled in deoxygenated, concentrated hemolysates by reversible, thermally dependent gel-sol transformations. Under the conditions of the Murayama Test such a demonstration is specific for S hemoglobin or the S structural variant, hemoglobin C (Harlem). The test: (1) is simple; (2) has clear end points; (3) will detect both homozygous and heterozygous S hemoglobin; and (4) is specific. The technique of the test is recorded. The specificity of the Murayama Test is shown to reside in particular molecular structural features of the hemoglobin tetramers of S, C (Harlem), C (Georgetown), and I (all sickling hemoglobinopathies). Data from 29 cases of S hemoglobin and 37 controls are reported. The close agreement between Murayama's hypothesis for sickling in S hemoglobin and the experimental data and analysis presented in this report lends additional support for his views. (Author)
Sickling Reversed and Blocked by Urea in Invert Sugar: Optical and Electron Microscopy Evidence( Book )

1 edition published in 1970 in English and held by 1 WorldCat member library worldwide

Optical and electron microscopic evidence is presented to support the finding that sickling of hemoglobin S can be reversed and blocked by urea in invert sugar (UIS). Erythrocytes from subjects having hemoglobin SS, AS, or AA were treated with UIS either before or after deoxygenation with sodium metabisulfite (Na2S2O5). Light microscopy studies indicated that approximately one-fifth as much urea is required to block sickling than is necessary to reverse previously sickled poikilocytes to normal forms. Intracellular microfilaments apparent in transmission electron micrographs of sickled erythrocytes were eliminated by treatment of aliquots of the same deoxygenated erythrocytes with UIS. Scanning electron micrographs showed a reversion of sickled poikilocytes to a normal erythrocyte population of biconcave discs. The use of UIS was deduced from Murayama's recently modified hypothesis for the molecular mechanism of sickling which clearly implicates the role of hydrophobic bonds formed between the no. 6 Valine substitution of the beta-S globins and the alpha globins of interacting hemoglobin molecules. The use of UIS to arrest the formation of such hydrophobic bonds is advocated as an evident, safe, and effective therapeutic strategy to treat sickle is advocated as an evident, safe, and effective therapeutic strategy to treat sickle cell crisis. (Author)
Morphology of experimental venous thrombi in the rat by Raymond L Henry( )

1 edition published in 1961 in English and held by 1 WorldCat member library worldwide

Modified sickledex tube test : a specific test for S hemoglobin by Raymond L Henry( Book )

2 editions published in 1970 in English and held by 1 WorldCat member library worldwide

The murayama test by Robert M Nalbandian( Book )

1 edition published in 1970 in English and held by 0 WorldCat member libraries worldwide

 
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Audience Level
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Audience level: 0.43 (from 0.10 for Morphology ... to 0.99 for The Muraya ...)

Languages
English (42)