WorldCat Identities

Hartley, Rebecca S.

Overview
Works: 5 works in 12 publications in 1 language and 650 library holdings
Genres: Academic theses 
Roles: Author
Classifications: HC286.7, 303.48243073
Publication Timeline
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Most widely held works by Rebecca S Hartley
United Germany and the United States by Michael A Freney( Book )

4 editions published in 1991 in English and held by 259 WorldCat member libraries worldwide

The Growth of regional trading blocs in the global economy( Book )

2 editions published in 1990 in English and held by 218 WorldCat member libraries worldwide

European and American labor markets : different models and different results( Book )

4 editions published in 1992 in English and held by 168 WorldCat member libraries worldwide

United Germany and the United States by Michael A Freney( Book )

1 edition published in 1991 in English and held by 3 WorldCat member libraries worldwide

Characterization of human NT2N neurons for transplantation therapeutics by Rebecca S Hartley( )

1 edition published in 1998 in English and held by 2 WorldCat member libraries worldwide

Stroke, Parkinson's Disease and spinal cord injury may be therapeutically treated with neuronal replacement strategies which could allow synaptically regulated delivery and circuit replacement. The NT2 cell line is the only human cell line capable of generating a pure reproducible neuronal population (NT2N neurons). I assessed the suitability of NT2N neurons for use in therapeutic transplantation. I implanted NT2N neurons into murine spinal cords and investigated their phenotypic stability and the patterning, connectivity and myelination of their process outgrowth. The effects of primary CNS cells on NT2N phenotype and the functional synaptic properties of NT2N neurons were investigated in vitro. The dopaminergic phenotype and manipulatability was examined in vitro and after CNS implantation. I found that grafted NT2N neurons fully integrate into spinal cord while maintaining a stable intrinsic phenotype. NT2N process outgrowth follows host anatomic patterns, is myelinated by the host, extends for distances $>$2 cm, and can grow within spinal nerves. Axonal morphology and synaptophysin expression suggest synaptic contacts between graft and host cells. In vitro, NT2N neurons have a manipulatable dopaminergic phenotype which is lost through mechanical disturbance. They form functional GABAergic and glutamatergic synapses. Co-culture with primary astrocytes affects NT2N morphology, survival, maturation, and synaptic function in a contact-dependent manner. I conclude that graft integration with host tissue is not dependent on neuronal progenitor cells nor on the presence of transplanted non-neuronal cells. Post-mitotic NT2N neurons are capable of phenotypic change as they differentially respond to astrocytes cultured from different CNS regions. This also indicates regional distinctions between CNS astrocytes. The lack of phenotypic effect of the host on implanted NT2N neurons appears to be due to the lack of direct contact between host and graft cells. NT2N neurons are similar to primary neurons in synaptogenesis and their survival and maturation requirements for glial support. The selective dopaminergic sensitivity to damage makes them attractive for investigations into dopaminergic phenotype and for Parkinson's Disease therapeutics. They might also be used to develop strategies for spinal cord injury treatment and to investigate the neurobiology of human neuronal transplants and for studies of neuronal development and neuron/glia interactions
 
Audience Level
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Audience Level
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Audience level: 0.55 (from 0.52 for United Ger ... to 0.95 for United Ger ...)

Languages
English (12)