WorldCat Identities

Rapley, Ralph

Overview
Works: 26 works in 289 publications in 2 languages and 9,364 library holdings
Genres: Laboratory manuals  Handbooks and manuals  Conference papers and proceedings 
Roles: Author, Editor, Other, Publishing director, Contributor
Publication Timeline
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Most widely held works by Ralph Rapley
Molecular biology and biotechnology by Walker. J. M( Book )

46 editions published between 2000 and 2015 in English and held by 823 WorldCat member libraries worldwide

This fifth edition has been revised and updated to reflect the developments in this rapidly expanding field. Chapters on the impact of molecular biology in the development of biotechnology have been fully updated and include the applications of molecular biology in the areas of drug design and diseases
Molecular biomethods handbook by John M Walker( Book )

36 editions published between 1994 and 2008 in English and held by 503 WorldCat member libraries worldwide

Numerous key protein methods, as well as support and related techniques, are also included."--Jacket
Molecular forensics by Ralph Rapley( Book )

10 editions published in 2007 in English and held by 381 WorldCat member libraries worldwide

Molecular Forensics offers comprehensive coverage of the increasingly important role molecular analysis plays within forensic science. Starting with a broad introduction to modern forensic molecular technologies, the text covers key issues from the initial scenes of crime sampling to the use of evidential material in the prosecution of legal cases. The book also explores the questions raised by the growing debate on the applications of national DNA databases and the resulting challenges of developing, maintaining and curating such vast data structures. The broader range of applications to non-human cases is also discussed, as are the statistical pitfalls of using so called unique data such as DNA profiles and the ethical considerations of national DNA databases. This book is an invaluable reference work for students taking courses within the Forensic and Biomedical sciences. It is also useful for practitioners in the field looking for a broad overview of the subject
The nucleic acid protocols handbook by Ralph Rapley( Book )

18 editions published between 1999 and 2008 in English and held by 284 WorldCat member libraries worldwide

A comprehensive treasury of all the key molecular biology methods-ranging from DNA extraction to gene localization in situ-needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular BiologyTM series, providing an introduction to the scientific basis of each technique, a complete listing of all the necessary materials and reagents, and clear step-by-step instruction to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate m
Molecular analysis and genome discovery by Ralph Rapley( Book )

35 editions published between 2004 and 2012 in English and held by 277 WorldCat member libraries worldwide

Provides a comprehensive overview of recent developments in the area of molecular based diagnostics (including nucleic acids, biosensors and immunoessays) of disease markers. It also covers the impact of techniques such as in vitro nucleic acid amplifications (e.g. PCR) and other amplification methods, as well as gene and biochip production and automated techniques such as fluorescent sequencing. The book discusses key concepts where new and merging areas, including pharmacogenomics, proteomics and functional genomics, are being researched and developed. In addition, examples are given where this new area of bioscience has or may be successfully applied
PCR sequencing protocols by Ralph Rapley( Book )

18 editions published in 1996 in English and held by 259 WorldCat member libraries worldwide

A superb, comprehensive collection of the most reliable protocols for PCR sequencing - all developed and described by eminent researchers and scientists who use them successfully every day. These protocols deal practically with those problems accompanying the rapid reassociation of amplified complementary strands by showing how sequencing reactions may be effectively modified, as well as complex methods of primer manipulation that enable the affinity purification, or its transcript production, of homogeneous single strands. They also provide all the necessary details of PCR purification, primer labeling, the preparation of sequencing gels, as well as of conventional methods for cloning PCR products into vectors and their subsequent sequencing
RNA isolation and characterization protocols by Ralph Rapley( Book )

18 editions published between 1997 and 1998 in English and Undetermined and held by 255 WorldCat member libraries worldwide

RNA Isolation and Characterization Protocols offers biological investigators a powerful and highly useful collection of up-to-date methods for the isolation of RNA from a variety of sources, including bacterial, plant, and mammalian cells. These fine-tuned protocols take both the experienced and inexperienced investigator through the nuances of RNA manipulation, from extraction to in vitro translation. The authoritative researchers writing here also include detailed protocols for the analysis and characterization of isolated RNA species and extensive troubleshooting hints and tips to ensure easy reproducibility. RNA Isolation and Characterization Protocols gives today's bench scientists the single best collection of easily reproducible, step-by-step RNA techniques now available. This book will quickly become indispensable for everyone seeking to manipulate RNA and help set the stage for the coming revolution in RNA-based diagnostic techniques
PCR mutation detection protocols( Book )

9 editions published in 2002 in English and held by 193 WorldCat member libraries worldwide

Application of DNA technology to the identification of disease-causing mutations has become widespread in recent years. PCR Mutation Detection Protocols, provides biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g., ASO-PCR, SSCP, DGGE, chemical cleavage), deletions (multiplex PCR, FISH, blotting), non-sense mutations (PTT), and more. The new and exciting techniques of DNA array analysis, along with such recently developed experimental methods as conformation-sensitive gel electrophoresis, are also included. Additional coverage is given to the direct use of DNA sequencing as a detection method in its own right and to the characterization of mutations previously located by other screening techniques. Each chapter explains the basic theory behind the technique and provides valuable notes essential for its successful execution. Comprehensive and highly practical, PCR Mutation Detection Protocols assures both seasoned and novice investigators access to the highly productive and readily reproducible PCR-based mutation detection methods, techniques that are laying the groundwork for many of today's major scientific and medical advances
Gene probes : principles and protocols by Marilena Aquino de Muro( Book )

11 editions published between 2001 and 2002 in English and held by 184 WorldCat member libraries worldwide

The use of nucleic acid or gene probes as cloning or diagnostic tools has promoted significant advances in human genome mapping and in the isolation and manipulation of genes generally. In Gene Probes, Marilena Aquino de Muro and Ralph Rapley have brought together an outstanding collection of time-tested protocols for designing and using gene probes in a wide variety of applications. The applications covered range from searching for specific genes in the human chromosome to the detection of microorganisms and their toxins in the environment and in food samples, as well as in the diagnosis of human disease. Each cutting-edge method includes practical hints and tips and is described in step-by-step detail by an expert who not only routinely uses the technique, but has also optimized it for experimental success. Helpful tutorials explain the principles of gene probe design, labeling, detection, target formation, and hybridization conditions. Comprehensive and versatile, Gene Probes: Principles and Protocols provides a cutting-edge practical resource for all biochemists, microbiologists, and molecular biologists who are using this powerful new technology to advance medical, environmental, and food research today
Route maps in gene technology by Matthew R Walker( Book )

18 editions published between 1997 and 2009 in English and held by 181 WorldCat member libraries worldwide

Medical biomethods handbook by John M Walker( Book )

14 editions published between 2005 and 2008 in English and held by 178 WorldCat member libraries worldwide

"Powerful new techniques in molecular biology have revolutionized our ability to investigate and define cellular processes at the molecular level for medical purposes. In Medical Biomethods Handbook, John Walker and Ralph Rapley have collected a wide-ranging group of molecular and biochemical techniques that are the most frequently used in medical and clinical research, especially diagnostics. The authors - well-established investigators who run their own research programs and use the methods on a regular basis - outline the practical procedures for using them and describe a variety of pertinent applications. Among the technologies presented are Southern and Western blotting, electrophoresis, PCR, cDNA and protein microarrays, liquid chromatography, in situ hybridization, karyotyping, flow cytometry, bioinformatics, genomics, and ribotyping. The applications include assays for mutation detection, mRNA analysis, chromosome translocations, inborn errors of metabolism, protein therapeutics, and gene therapy." "State-of-the-art and highly practical, Medical Biomethods Handbook offers novice and experienced researchers an in-depth understanding of the theory, practice, and application of the key molecular biology techniques used in biomedical research and diagnostic investigation today."--Jacket
Molecular diagnostics( Book )

5 editions published in 1993 in English and held by 124 WorldCat member libraries worldwide

Molecular and antibody probes in diagnosis by Matthew R Walker( Book )

5 editions published in 1993 in English and held by 120 WorldCat member libraries worldwide

Molecular and cellular therapeutics by David Whitehouse( Book )

14 editions published in 2012 in English and held by 105 WorldCat member libraries worldwide

Molecular and Cellular Therapeutics aims to bring together key developments in the areas of molecular diagnostics, therapeutics and drug discovery. The book covers topics including diagnostics, therapeutics, model systems, clinical trials and drug discovery. The developing approaches to molecular and cellular therapies, diagnostics and drug discovery are presented in the context of the pathologies they are devised to treat
PCR mutation detection protocols by Bimal D. M Theophilus( Book )

15 editions published between 2002 and 2011 in English and held by 66 WorldCat member libraries worldwide

Since the publication of the popular first edition, the explosion of DNA sequence information, the access to bioinformatics and mutation databases coupled with the ability to readily detect and confirm mutations has cemented the role of molecular diagnostics in medicine and, in particular, mutation detection by the polymerase chain reaction (PCR). In PCR Mutation Detection Protocols, Second Edition, expert researchers bring the subject up-to-date with key protocols involving the PCR and its many various incarnations such as SSCP, CSGE, and dHPLC. The volume also addresses key areas such as Southern blotting, accurate diagnostics with high throughput, as well as microarray systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes which provide the often hard to find information that may mean the difference between the success and failure of the method. Authoritative and cutting-edge, PCR Mutation Detection Protocols, Second Edition aims to stimulate postgraduate scientists, researchers, and clinicians already engaged in the area and to provide an important first step for those new to this practice wanting to adopt the powerful and essential technique in their own laboratories
Molecular biomethods handbook by Ralph Rapley( Book )

5 editions published between 1997 and 2008 in English and held by 9 WorldCat member libraries worldwide

Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. Molecular Biomethods Handbook, 2nd Edition expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Part A of the book describes nucleic acid methods, such as gene expression profiling, microarray analysis and quantitative PCR
Guia de rotas na tecnologia do gene by Matthew R Walker( Book )

1 edition published in 1999 in Portuguese and held by 2 WorldCat member libraries worldwide

Monoclonal antibodies( )

in Undetermined and held by 2 WorldCat member libraries worldwide

Molecular analysis and manipulation of antibody variable region genes by Ralph Rapley( )

1 edition published in 1992 in English and held by 1 WorldCat member library worldwide

Gene Probes : Principles and Protocols. Methods in Molecular Biology, Volume 179( )

2 editions published in 2002 in English and held by 0 WorldCat member libraries worldwide

The use of nucleic acid or gene probes as cloning or diagnostic tools has promoted significant advances in human genome mapping and in the isolation and manipulation of genes generally. In Gene Probes, Marilena Aquino de Muro and Ralph Rapley have brought together an outstanding collection of time-tested protocols for designing and using gene probes in a wide variety of applications. The applications covered range from searching for specific genes in the human chromosome to the detection of microorganisms and their toxins in the environment and in food samples, as well as in the diagnosis of human disease. Each cutting-edge method includes practical hints and tips and is described in step-by-step detail by an expert who not only routinely uses the technique, but has also optimized it for experimental success. Helpful tutorials explain the principles of gene probe design, labeling, detection, target formation, and hybridization conditions. Comprehensive and versatile, Gene Probes: Principles and Protocols provides a cutting-edge practical resource for all biochemists, microbiologists, and molecular biologists who are using this powerful new technology to advance medical, environmental, and food research today
 
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Molecular forensics
Alternative Names
Rapley, Ralph

Raply, Ralph

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Covers
Molecular biomethods handbookMolecular forensicsThe nucleic acid protocols handbookMolecular analysis and genome discoveryPCR sequencing protocolsRNA isolation and characterization protocolsPCR mutation detection protocolsGene probes : principles and protocols