Orgogozo, Virginie
Works: | 17 works in 46 publications in 2 languages and 565 library holdings |
---|---|
Genres: | Laboratory manuals Dictionaries Bibliographies |
Roles: | Editor, Author, Other, Opponent, htt, Thesis advisor |
Classifications: | QH390, 572.838 |
10 editions published between 2011 and 2016 in English and held by 236 WorldCat member libraries worldwide
We are entering a particularly fruitful period in evolutionary genetics, as rapid technological progress transforms the investigation of genetic variation within and between species. Molecular Methods for Evolutionary Genetics is a collection of advanced molecular biology protocols and general overviews intended to represent the essential methods currently bringing evolutionary genetics to fruition. Divided into six thematic sections, this volume covers methods for characterizing genomes, diverse approaches to enrich DNA for subsets of the genome prior to sequencing, and state-of-the-art protocols for sampling genetic variation for genetic mapping studies and population genetic studies (RAD sequencing, Sequenom, microarrays, etc.). The volume concludes by focusing on methods to study candidate genes, from obtaining their sequences and analyzing their transcripts to experimentally manipulating their activities in vivo. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Molecular Methods for Evolutionary Genetics serves as a rich resource to biologists interested in evolution, whether they be specialists or beginners in molecular biology
13 editions published in 2016 in English and held by 229 WorldCat member libraries worldwide
"This volume of Current Topics in Developmental Biology is being published at a time when many research laboratories are focusing on the evolution of diverse phenotypic traits and trying to dissect their genetic basis. A wide array of powerful methods have been developed in recent years to find the genes and the mutations responsible for evolutionary changes, so that empirical data on the "loci of evolution" are accumulating at a fast pace. The field of evolutionary genetics has now moved from pure theoretical computations of changes in allele frequencies in populations to assessments of the actual mutations that occurred in populations and that cause observable changes in phenotype, bringing up new ideas and new questions. Each chapter provides an overview of current knowledge and ongoing research on the genes and the mutations responsible for phenotypic evolution in different areas of investigation. As a whole this volume constitutes a remarkable material to reflect on our current state of research and to try to detect new future explanatory frameworks and ideas to be discovered from our examination of the genes and the mutations responsible for evolution"--
1 edition published in 2020 in French and held by 60 WorldCat member libraries worldwide
"À un moment où les revendications identitaires sont légion, il faut revenir en amont d'une tendance qui galvaude un concept philosophique pour le mobiliser sur le seul terrain idéologique et politique. Qui suis-je? Aucune discipline scientifique n'oserait à elle seule penser, affronter et circonscrire cette vieille question métaphysique... et enfantine. En mettant en œuvre une interdisciplinarité effective, les auteurs ont pour ambition d'éclairer l'énigme de l'identité personnelle, mais pas seulement. En effet, l'identité est à la fois le caractère de ce qui est même et de ce qui est unique, qu'importe l'objet. Pensée comme individuelle, elle serait tour à tour personnelle, psychologique, génétique ou narrative; pensée comme collective, elle serait sociale, ethnique, familiale, genrée, linguistique ou encore nationale. À travers les différents regards exposés ici, le lecteur tracera son propre chemin dans les méandres de cette notion."--
6 editions published in 2011 in English and held by 18 WorldCat member libraries worldwide
We are entering a particularly fruitful period in evolutionary genetics, as rapid technological progress transforms the investigation of genetic variation within and between species. Molecular Methods for Evolutionary Genetics is a collection of advanced molecular biology protocols and general overviews intended to represent the essential methods currently bringing evolutionary genetics to fruition. Divided into six thematic sections, this volume covers methods for characterizing genomes, diverse approaches to enrich DNA for subsets of the genome prior to sequencing, and state-of-the-art protocols for sampling genetic variation for genetic mapping studies and population genetic studies (RAD sequencing, Sequenom, microarrays, etc.). The volume concludes by focusing on methods to study candidate genes, from obtaining their sequences and analyzing their transcripts to experimentally manipulating their activities in vivo. Written in the highly successful Methods in Molecular Biology"!series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Molecular Methods for Evolutionary Genetics serves as a rich resource to biologists interested in evolution, whether they be specialists or beginners in molecular biology
3 editions published in 2016 in English and held by 4 WorldCat member libraries worldwide
This volume of Current Topics in Developmental Biology is being published at a time when many research laboratories are focusing on the evolution of diverse phenotypic traits and trying to dissect their genetic basis. A wide array of powerful methods have been developed in recent years to find the genes and the mutations responsible for evolutionary changes, so that empirical data on the "loci of evolution" are accumulating at a fast pace. The field of evolutionary genetics has now moved from pure theoretical computations of changes in allele frequencies in populations to assessments of the actual mutations that occurred in populations and that cause observable changes in phenotype, bringing up new ideas and new questions. Each chapter provides an overview of current knowledge and ongoing research on the genes and the mutations responsible for phenotypic evolution in different areas of investigation. As a whole this volume constitutes a remarkable material to reflect on our current state of research and to try to detect new future explanatory frameworks and ideas to be discovered from our examination of the genes and the mutations responsible for evolution"--Publisher description
2 editions published in 2003 in French and held by 4 WorldCat member libraries worldwide
1 edition published in 2019 in English and held by 2 WorldCat member libraries worldwide
1 edition published in 2019 in English and held by 2 WorldCat member libraries worldwide
1 edition published in 2016 in English and held by 2 WorldCat member libraries worldwide
1 edition published in 2018 in French and held by 1 WorldCat member library worldwide
La couleur est un trait biologique essentiel et très variable qui permet à un organisme d'être adapté à son environnement biotique et abiotique. De multiples gènes influent sur la couleur ; leur évolution a été étudiée ici chez les Vertébrés en lien avec les duplications de génome survenues dans cette lignée . Alors que l'essentiel des gènes sont perdus lors de la rediploïdisation suivant une duplication totale de génome, ces travaux montrent que les gènes de la pigmentation appartiennent à des fa milles de gènes qui sont en moyenne plus retenues que le reste du génome . Ceci est particulièrement vrai pour les poissons Téléostéens, cette expansion du répertoire de gènes de pigmentation pouvant être en lien avec la grande diversité de cellules pigmenta ires et de patrons de pigmentation observés chez ces animaux . Par ailleurs, l'étude de la pigmentation d'un organisme modèle émergent, le poisson -clown Amphiprion oellaris, a été réalisée dans le cadre de cette thèse . La nature cellulaire de la bande blanche de ce poisson a été déterminée et a permis l'identification - d'un nouveau gène présent presque exclusivement chez les poissons Actinoptérygiens , probablement acquis par transfert horizontal, et impliqué dans le développement d'un type de cellules pigmentaires, les iridophores . Enfin, l'acquisition de la pigmentation au cours du développement du poisson -clown A. ocellaris, un poisson corallien au cycle de développement marqué par une métamorphose, a été étudiée en rapport avec la signalisation thyroïdienne, qui joue un rôle majeur au cours du développement des Vertébrés
1 edition published in 2021 in English and held by 1 WorldCat member library worldwide
Bioadhesives are a great natural resource for innovations and characterizing mechanisms that are involved in bioadhesion process could help developing new biocompatible and biodegradable materials with novel properties. Drosophila flies produce a glue at the end of the larval stage which allows the pupa to stay firmly attach to a substrate during metamorphosis. Although D. melanogaster has been extensively studied in the laboratory, little is known about the role and the evolution of fly glue. During my thesis,I aimed at better characterizing the properties, the function as well as the evolution of Drosophila glue. We first designed a simple pull-off force test which allows us to measure the adhesion of individual pupae. Using this test, we showed that D. melanogaster glue interacts with polarizable substrates and that Drosophila glue adhesion may rely on non covalent interactions between charged surfaces. Then, we found that the glue protects pupa from being collected by ant predators. Drosophila glue thus represents a crucial trait for fly survival.We observed that adhesion varies between but also within species originating from different locations. To better characterize the evolution of the glue genes, we analyzed the sequences of several Drosophila genomes for which the assembly within the repeat regions is good. Our study revealed that the eight glue genes evolved differently. In particular,Sgs1, Sgs3, Sgs7 and Sgs8 display low diversity in D. melanogaster whereas Sgs5 andSgs5bis show high diversity which may be due to local adaptations in these lines. UsingRNAi lines to specifically inactivate the expression of each glue genes, we provide the first evidence that Sgs1 and Sgs3 contribute to the adhesive properties of Drosophile glue. Finally, in the species Megaselia scalaris, our experiments suggest that the fluid produced by their salivary glands is not involved in the adhesion of the pupa but in defense against larva's predators. My thesis work allows to develop a new promising model for the study of species adaptation: Drosophila glue. Furthermore, it paves the way for future works aiming at deciphering the mechanisms of bioadhesion
1 edition published in 2018 in English and held by 1 WorldCat member library worldwide
1 edition published in 2020 in English and held by 1 WorldCat member library worldwide
Les animaux utilisent les signaux de leur environnement pour guider leurs comportements. De nombreux comportements cruciaux, tel que le choix du site de ponte chez les insectes, sont le résultats d'adaptation à divers signaux. Un même signal sensoriel peut être perçu et interprété différemment par deux espèces, mais les mechanismes responsables de l'evolution du comportement sont encore mal-connus. Dans la nature, la majorité des Drosophiles préfèrent pondre dans les fruits en décomposition. A l'inverse, D. suzukii préfère pondre dans les fruits mûrs. Ce comportement spécifique a fait de D. suzukii un ravageur de culture important. Le changement de préférence de ponte de D. suzukii du fruit pourri vers le fruit mûr est une opportunité pour étudier les mechanismes de l'evolution du comportement. Mon projet de thèse vise à identifier les signaux gustatifs et les composants du système sensoriel périphérique (récepteurs, neurones) impliqués dans le comportement de ponte de D. suzukii. Dans les fruits mûrs, les sucres sont présents en abondance, et pourraient être un signal chimique important pour guider la préférence de ponte de D. suzukii.Pour répondre à cette hypothèse, j'ai utilisé une approche comparative entre D. suzukii et D. melanogaster incluant (1) des test comportementaux de ponte variés et (2) l'établissement du profile transcriptomique des organes gustatifs.Ensemble, mes résultats suggèrent que la préférence de ponte de D. suzukii pour les fruits mûrs pourrait être guidée par sa forte préférence pour le fructose et le glucose. Des changements importants dans le pool des GRs pourraient être à l'origine de cette plus forte réponse aux sucres des fruits
1 edition published in 2017 in English and held by 1 WorldCat member library worldwide
The thesis tackles the problem of the evolution of shape through the example of a shape difference in the male ventral branches linked to reproductive isolation in two sister species: Drosophila yakuba and Drosophila santomea. The goal is to identify the genes involved in the evolution of this shape difference and the evolutionary causes of such difference. In a first part, the thesis interrogates the concept of “gene” and its search. Then are scientifically characterized the “how” and “why” questions and their link with the distinction of proximal/ultimate, or evolutionary, causes; these philosophical grounds are then linked to Evo-Devo and the experimental work presented in the thesis. In a second part, through geometric morphometrics and a new high-throughput genotyping method, MSG, we identify a loci of 2.7 mega-bases located on chromosome 3L as involved in the evolution of the shape of ventral branches between D. yakuba and D. santomea. These results are linked to our quantitative analysis of shape variation in multiple natural and laboratory strains and strains reared at different temperatures which bring light into the evolutionary causes of this shape difference
1 edition published in 2019 in Undetermined and held by 1 WorldCat member library worldwide
Animal terminalia represent some of the most diverse and rapidly evolving structures in the animal kingdom, and for this reason have been a mainstay in the taxonomic description of species. The terminalia of Drosophila melanogaster, with its wide range of experimental tools, have recently become the focus of increased interest in the fields of development, evolution, and behavior. However, studies from different disciplines have often used discrepant terminologies for the same anatomical structures. Consequently, the terminology of genital parts has become a barrier to integrating results from different fields, rendering it difficult to determine what parts are being referenced. We formed a consortium of researchers studying the genitalia of D. melanogaster to help establish a set of naming conventions. Here, we present a detailed visual anatomy of male genital parts, including a list of synonymous terms, and suggest practices to avoid confusion when referring to anatomical parts in future studies. The goal of this effort is to facilitate interdisciplinary communication and help newcomers orient themselves within the exciting field of Drosophila genitalia
1 edition published in 2016 in English and held by 1 WorldCat member library worldwide
in English and held by 1 WorldCat member library worldwide


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General | Special |

- Rockman, Matthew V. Editor
- Villa, François
- Gayon, Jean
- Weitzman, Jonathan
- Pontarotti, Gaëlle 1984-
- Nicoglou, Antonine 1983-
- Troubé, Sarah 1983-
- SpringerLink (Online service) Other
- Schweisguth, François (1963-....). Thesis advisor
- Université Pierre et Marie Curie (Paris / 1971-2017) Degree grantor