WorldCat Identities

Bigot, Yves 1961-

Works: 17 works in 21 publications in 2 languages and 226 library holdings
Genres: Laboratory manuals 
Roles: Thesis advisor, Other, Opponent
Publication Timeline
Most widely held works by Yves Bigot
Mobile genetic elements : protocols and genomic applications by Yves Bigot( )

3 editions published in 2012 in English and held by 199 WorldCat member libraries worldwide

Transposable elements are used as genetic tools for dissecting the function of a specific gene and elaborating on mechanisms leading to genetic change and diversity, and the evolutionary impact of mobile DNA on the biology and evolution of organism. In, Mobile Genetic Elements: Protocols and Genomic Applications, Second Edition gathers a wide array of strategies and protocols for identifying transposable elements (TEs) and their evolutionary derivatives, and for studying genome structure, function, and evolution. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Through and intuitive Mobile Genetic Elements: Protocols and Genomic Applications, Second Edition aids scientists in the continuing study of in silico tools, strategies and protocols that are complementary of those described in the first volume
Three non-autonomous signals collaborate for nuclear targeting of CrMYC2, a Catharanthus roseus bHLH transcription factor by Sabah Hedhili( )

1 edition published in 2010 in English and held by 2 WorldCat member libraries worldwide

Sequence properties of certain GC rich avian genes, their origins and absence from genome assemblies: case studies by Linda Beauclair( )

1 edition published in 2019 in English and held by 2 WorldCat member libraries worldwide

Mariner Mos1 transposase optimization by rational mutagenesis by Stéphanie Germon( )

1 edition published in 2009 in English and held by 2 WorldCat member libraries worldwide

But where did the centromeres go in the chicken genome models? by Benoît Piégu( )

1 edition published in 2018 in English and held by 2 WorldCat member libraries worldwide

Complete genome sequence of invertebrate iridovirus IIV22A, a variant of IIV22, isolated originally from a blackfly larva by Benoît Piégu( )

1 edition published in 2014 in English and held by 2 WorldCat member libraries worldwide

Transposon tools: worldwide landscape of intellectual property and technological developments by Fabien Palazzoli( )

1 edition published in 2009 in English and held by 2 WorldCat member libraries worldwide

An Assessment of Fixed and Native Chromatin Preparation Methods to Study Histone Post-Translational Modifications at a Whole Genome Scale in Skeletal Muscle Tissue by Sarah-Anne David( )

1 edition published in 2017 in English and held by 2 WorldCat member libraries worldwide

Mise au point d'un système dérivé du transposon Mos1 comme vecteur non viral de transfert de gène en cellules eucaryotes by Gwenhael Jegot( Book )

2 editions published in 2007 in French and held by 2 WorldCat member libraries worldwide

The mariner Mos 1 transposon can naturally move into the genome. The first research axis of this work concern the transposon characteristics which act upon the transposition activity of Mos1 (like the nucleic content of the transposon and its size). It have shown that a size of transgene upper than 2,5 kb limit the transposition efficiency, that a strong GC percentage favour the transposition, and that there is no minimal size for Mos1 transposition. The second research axis concern the adaptation and improvement of the Mos1 system to the eukaryotic cells, to developp a nonviral vector for gene transfer. The addition of a nuclear localization sequence improve the nuclear transfer, the "humanization" of the transposase sequence increase it expression in mammal cells, and the use of the HSVtK suicide system permit to limit the recombination events
The mariner Transposons Belonging to the irritans Subfamily Were Maintained in Chordate Genomes by Vertical Transmission by Ludivine Sinzelle( )

1 edition published in 2006 in English and held by 2 WorldCat member libraries worldwide

Factors acting on Mos1 transposition efficiency by Ludivine Sinzelle( )

1 edition published in 2008 in English and held by 2 WorldCat member libraries worldwide

Caractérisation du génome des ascovirus et de leurs relations phylétiques avec les autres familles de virus à grand génome ADN double brin by Karine Stasiak( Book )

2 editions published in 2001 in French and held by 2 WorldCat member libraries worldwide

This work describes the molecular analysis of ascovirus. The first part presents the study of the ascovirus genome. This genome consists of a double-stranted circular partially supercoiled DNA molecule, which contains large interspersed repeats. Repeated genes of the bro family (baculovirus repeated open reading frame) have also been discovered. The study revealed also that ascovirus genome is methylated on cytosines positions, with DpAV-4a having the highest level of methylation reported for a DNA virus (76%). In a second part, phylogenetic analysis were done on ascovirus genome. The first phylogenetic analysis were base on 4 different genes : the genes of the DNA polymerase, of the major capside protein (MCP), of the thymidine kinase (TK), and of the ATPase III. All of them revealed that ascoviruses are closely related to iridoviruses, and also, to a less extent, to phycodnaviruses and asfarviruses. Moreover, two of them, TK and ATPase III genes, indicate that ascoviruses have evolved form invertebrate iridoviruses. This last observation is confirmed with other genes which are specifically present in this two viral families. Finally, other phylogenetic analyses were done to study the putative evolutionary relationship between Ascoviridae and ichnoviridae. The pylogenetic analysis done on the MCP gene provides evidence that proteins involved in the ichnovirus capsid are related to ascovirus capsid proteins, and likely have evolved from them. On the other hand, no other ichnoviral gene have a homologue in ascovirus or iridovirus genomes. This observation suggests that ichnovirus particles could be a viral capsid used by the wasp to transfert its own genes in its host
Etude de l'organisation du génome de poulet à travers les séquences répétées by Sébastien Guizard( )

1 edition published in 2016 in French and held by 1 WorldCat member library worldwide

The genomes of avian species have special features such as the structure of chromosomes or their content in repeated sequences. Indeed, compared to vertebrate genomes in which the amount of repetitions varies from 30 to 55%, it is lower in avian species and varies from 8 to 10%. The annotation of repeated content is most often done with the RepeatMasker program that is generally use the Repbase database of repeated sequences. This kind of approach is based solely on the sequence of already known transposable elements. In fact, this program is not able to detect new repeats and in consequence produced annotations with a quality that depends on the sequences of transposable elements used. More and more studies show that transposable elements play a role in the functioning of the genome and can influence gene expression. It is therefore essential that the annotation of these sequences is as complete as possible. There are many programs using methods for detecting de novo transposable elements, either by searching for characteristic structures, or by comparing the genome against itself. However, no standard strategy of annotation for repeated sequences have been defined yet. My thesis aims to set-up a standard strategy of annotation for repeated sequences that was applied to a large genome, that of the red jungle fowl. The obtained annotation allowed me studying the genome organization in this species through its repeated sequences and transposable elements
Caractérisation des sites d'insertion du transposon mariner Mos 1 by Gwénaëlle Crenes( )

1 edition published in 2009 in French and held by 1 WorldCat member library worldwide

L'élément mariner Mos1 est un élément mobile de classe II, sans spécificité d'insertion évidente, hormis un dinucléotide TA dupliqué lors de l'insertion. Pourtant, des travaux antérieurs révèlent l'existence de deux sites préférentiels d'insertion : le gène bactérien cat, codant une chloramphénicol acétyl-transférase et le locus du rDNA chez Caenorhabditis elegans. Le premier objectif de ce travail a été d'évaluer l'attractivité du gène cat dans différents contextes plasmidiques et chromosomiques, par des tests in vitro et en bactéries. Le second objectif était de faire le point sur les sites d'insertions de l'élément Mos1 à partir des données disponibles in vitro et in vivo. Une analyse statistique a révélé que l'insertion de Mos1 était préférentiellement ciblée dans des TA regroupés en motifs TATA ou TAxTA, ou localisés dans des régions riches en AT. Pour finir, le gène cat a servi de modèle pour déterminer que, si la courbure et la flexibilité de l'ADN n'expliquaient pas l'attractivité des sites préférentiels, la sélection du site d'insertion de Mos1 était dépendante de l'hélicité de la cible et que l'intervention de facteurs protéiques codés par l'hôte n'est pas à exclure
Conception d'un système protéique pour le ciblage de vecteurs non viraux au niveau des gènes codant les ARN ribosomiques by Elodie Carnus( )

1 edition published in 2009 in French and held by 1 WorldCat member library worldwide

The main challenge of gene transfer technologies is to maintain and to sustain transgene expression and to confer innocuity on the genetically-modified cells. Most integration systems, derived from transposons, integrate randomly within the genome of cells. The issue of this work is to develop tools to target transgene integrations in a selected locus in order to improve biosecurity. This study consists in using ZFD (Zinc Finger Domain) proteins for their capability to be in silico synthesize, in using many bioinformatic sites. For compare, the properties of two DNA binding domains (DBD), NterR2P, originating from the endonucleases encoded by R2 non-LTR retrotransposons, are able to bind specifically within a 100-bp region of the 28S rRNA genes. The results show that NterR2P DBDs specifically recognize their DNA target with high affinity. Two fusion proteins, using NterR2P DBD, are synthesized in order to integrate the transgene within rDNA, using Sleeping Beauty transposon. The original idea of this work is to realize transgene integration via indirect targeting of transposon, or transposase without its modification. The use of such a targeting system will have to be extensively studied to determine its impacts on cells before it can be considered as safe for use
Exploitation de l'information brevets dans un laboratoire de recherche public : identification de niches de développement technologique en bioproduction en en thérapie génique. by Fabien Palazzoli( )

1 edition published in 2011 in French and held by 1 WorldCat member library worldwide

In a world where the innovation race is increasing fast, it is of economic importance for an innovative company or a public research laboratory to develop a strategy for the protection and enhancement of its inventions is efficient Protection of results through patents is critical for the industrial development of biotechnology which are an innovative and promising sector where R&D requires considerable financial investments. Beyond this fundamental interest, patents are also a source of information on technological, legal and strategic, which can be exploited through patent landscapes. These studies are a key tool for decision supportin R&D since they allow to identify research strategies of competitors and technological niches free from of Intellectual Property rights
Etude génomique des mécanismes nucléaires de contrôle qualité et dégradation de l'ARN by Kevin Moreau( )

1 edition published in 2019 in French and held by 1 WorldCat member library worldwide

La transcription des ARN messagers (ARNm), chez les eucaryotes est un processus complexe nécessitant de nombreuses étapes. En parallèle de ce mécanisme fondamentale, de nombreuses protéines vont se fixer à l'ARNm naissant afin de le maturer et de l'empaqueter pour former une particule ribonucléoprotéique (mRNP) compétente pour l'export vers le cytoplasme, où aura lieu la traduction. Les étapes de biogénèse de cette mRNP sont soumises à la surveillance par un système de contrôle qualité (QC) qui détectera tout évènement conduisant à une mauvaise formation de la particule. Les transcrits aberrants sont retenus au noyau pour y être dégradés par l'exosome. Afin de provoquer l'apparition massive d'ARNm aberrants dans le noyau de notre modèle d'étude, la levure S. cerevisiae, nous utilisons le facteur bactérien Rho. L'hélicase translocase Rho, une fois exprimée, va venir perturber l'assemblage co-transcriptionnel des mRNPs et ainsi générer des transcrits aberrants qui seront substrats de la machinerie de QC et dégradation. La présente étude étend les précédentes observations faites par l'équipe quant à l'implication de certaines protéines dans le système de QC par des approches globales (RNA-seq, ChIP-seq). De plus, l'étude du complexe THO, impliqué dans l'empaquetage et le transport de la mRNP, révèle l'implication d'une de ses sous-unités dans le marquage des ARNm aberrants ainsi qu'un lien de première importance dans le recrutement de l'exonucléase Rrp6 sur ces cibles à dégrader. Enfin, nous montrons, de manière globale l'existence, au sein du noyau des levures, d'une seconde voie de dégradation des ARNs aberrants distincte de la voie canonique passant par Rrp6
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Mobile genetic elements : protocols and genomic applications
Alternative Names
Yves Bigot wetenschapper

English (12)

French (9)