WorldCat Identities

Bryant, Derek

Overview
Works: 2 works in 2 publications in 1 language and 7 library holdings
Roles: Author
Publication Timeline
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Most widely held works by Derek Bryant
Mentoring by Derek Bryant( Book )

1 edition published in 1998 in English and held by 6 WorldCat member libraries worldwide

Mesoscale modeling in biophysics: Applications to formin( )

1 edition published in 2016 in English and held by 1 WorldCat member library worldwide

Many actin structures are nucleated and assembled by the barbed-end tracking polymerase formin family, including filopodia, focal adhesions, the cytokinetic ring and cell cortex. These structures respond to forces in distinct ways. Formins typically have profilin-actin binding sites embedded in highly flexible disordered FH1 domains, hypothesized to diffusively explore space to rapidly capture actin monomers for delivery to the barbed end. Recent experiments demonstrate that formin mediated polymerization accelerates when under tension. The acceleration has been attributed to modifying the state of the FH2 domain of formin. Intriguingly, the same acceleration is reported when tension is applied to the FH1 domains, ostensibly pulling monomers away from the barbed end. Here we define an emerging mod-eling method, which we call mesoscale modeling, and develop a model of formin-mediated actin polymerization in the context of this method. In our model, we include monomer capture and delivery by FH1, which sterically interacts with actin along its entire length. The binding of actin monomers to their specific sites on FH1 is entropically disfavored by the high disorder. We find that this penalty is attenuated when force is applied to the FH1 domain by revealing the binding site, increasing monomer capture efficiency. Overall polymerization rates can decrease or increase with increasing force, depending on the length of FH1 domain and location of binding site. Our results suggest that the widely varying FH1 lengths and binding site locations found in known formins could be used to differentially respond to force, depending on the actin structure being assembled
 
Audience Level
0
Audience Level
1
  Kids General Special  
Audience level: 0.86 (from 0.84 for Mentoring ... to 0.99 for Mesoscale ...)

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