WorldCat Identities

Espéli, Marion

Overview
Works: 19 works in 34 publications in 2 languages and 277 library holdings
Genres: Laboratory manuals 
Roles: Editor, Opponent, Author, Thesis advisor, Publishing director, htt, Other
Classifications: QR185.8.T2, 616.0797
Publication Timeline
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Most widely held works by Marion Espéli
T follicular helper cells : methods and protocols by Marion Espéli( )

9 editions published in 2015 in English and held by 160 WorldCat member libraries worldwide

This volume brings together the skills and protocols of numerous laboratories that are at the heart of investigation into the biology of Tfh cells in both mice and humans. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Concise and easy-to-use, T follicular Helper Cells: Methods and Protocols provides scientist with techniques and protocols that have facilitated breakthroughs in Tfh biology and to present them in a way that will enable both new and experienced researchers to continue to move this exciting field forward
Bone marrow environment : methods and protocols by Marion Espéli( )

5 editions published between 2021 and 2022 in English and held by 95 WorldCat member libraries worldwide

This volume brings together classical and cutting-edge protocols on the spatio-temporal study of the cellular subsets constituting the bone and the marrow in both mouse and human. Chapters details methods on bone marrow (BM) ecosystem, to label, sort, analyse, and culture specific cell subsets as well as techniques allowing the evaluation of the function of some of the cellular elements of the BM. Written in the highly successfulMethods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bone Marrow Environment: Methods and Protocols aims to help new investigators to pursue the characterization of the BM microenvironment in the coming years
Bone Marrow Environment : Methods and Protocols by Marion Espéli( )

2 editions published in 2021 in English and held by 3 WorldCat member libraries worldwide

This volume brings together classical and cutting-edge protocols on the spatio-temporal study of the cellular subsets constituting the bone and the marrow in both mouse and human. Chapters details methods on bone marrow (BM) ecosystem, to label, sort, analyse, and culture specific cell subsets as well as techniques allowing the evaluation of the function of some of the cellular elements of the BM. Written in the highly successfulMethods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bone Marrow Environment: Methods and Protocols aims to help new investigators to pursue the characterization of the BM microenvironment in the coming years
<> by Marion Espéli( )

1 edition published in 2019 in English and held by 2 WorldCat member libraries worldwide

Influence du microenvironnement stromal de la moelle osseuse sur le développement des lymphocytes B normaux et pathologiques by Marielle Balzano-Foucher( )

2 editions published in 2016 in French and held by 2 WorldCat member libraries worldwide

In adults, the early stages of hematopoietic development take place in the bone marrow (BM). The contribution of specialized cells of mesenchymal origin, called stromal niches, has been demonstrated in the case of hematopoietic stem cell (HSC) maintenance and B lymphocyte development. Indeed, the maintenance of HSC depends on perivascular niches secreting CXCL12 and SCF. Furthermore progenitor B cells (preproB) are in contact with CXCL12+ stromal cells and migrate towards interleukin 7 expressing stromal cells during their differentiation into proB cells. PreBCR expression then marks the entrance into the preB cell stage. At this point, the cells are in contact with galectin-1+ stromal cells.Although progress have been made in understanding the role of stromal cell niches, their heterogeneity and the mechanisms controlling migration and adhesion of differentiating hematopoietic cells are controversial and remain to be defined. With this objective, we characterized phenotypically BM stromal cells but also demonstrated the existence of a multi-specific niche, associated to sinusoids and able to support both HSC and early B cells.The contribution of BM niches in the development and resistance to treatment of B cell Acute Lymphoblastic Leukemia (B-ALL), pathological equivalent of developing B cells has also been demonstrated. During my PhD, our work revealed the influence of a factor expressed by BM stromal cells on the proliferation of B-ALL. Ultimately, this work will allow the development of treatments targeting the protective functions of tumor niches
Analyse fonctionnelle des interactions entre cellules pré-B et cellules stromales de la moelle osseuse au cours de la différenciation B by Marion Espéli( Book )

2 editions published in 2007 in French and held by 2 WorldCat member libraries worldwide

Role of Eros, a novel transmembrane protein, in regulation of host defence( )

1 edition published in 2016 in English and held by 1 WorldCat member library worldwide

Abstract: Background: Reactive oxygen species (ROS), generated via the phagocyte NADPH oxidase cytochrome b558, are essential for effective immune responses to common and serious pathogens. The phagocyte NADPH oxidase is a multisubunit protein complex and deficiency of either the membrane bound or cytoplasmic components leads to chronic granulomatous disease, a serious and often fatal illness characterised by recurrent infections and autoimmunity. Moreover, abnormal generation of ROS has been implicated in the pathogenesis of multigenic autoimmune diseases such as systemic lupus erythematosus. Eros (essential for reactive oxygen species), encoded by bc017643, is a novel transmembrane protein that is highly expressed in the immune system and highly conserved in evolution but has no previously identified function. Eros is an orthologue of the plant protein Ycf4, necessary for expression of proteins of the photosynthetic photosystem 1 complex, an NADPH oxio-reductase complex. We elucidated its role in infection in mice. Methods: ROS are essential for host defence against the serious bacterial pathogen Salmonella enterica serovar Typhimurium. We screened individual knockout mice (Wellcome Trust Knockout mouse project) for susceptibility to salmonella infection. Having identified mice deficient in Eros as being highly susceptible to salmonella, we used ex-vivo approaches including reactive oxygen burst assays and western blot, to characterise their defect further. Findings: We found that Eros was essential for host defence to infection. Eros was crucial for generating reactive oxygen species through regulation of the essential NADPH oxidase components, gp91 and p22. Eros-deficient mice expressed almost no gp91 and p22 in neutrophils and macrophages secondary to accelerated degradation in the absence of Eros. As a result Eros-deficient mice died rapidly after infection with salmonella or listeria. Eros also regulated the ROS-dependent formation of neutrophil extracellular traps and melanoma metastases. Interpretation: We have found a a key role for Eros in regulating host defence. The finding that Eros-deficient mice lack gp91 and p22 at the protein, though not mRNA, level shows how these key components of the reatcive oxygen burst are protected from degradation and furthers our understanding of reactive oxygen burst biology. Eros is highly conserved between mouse and man so it is likely that it also has a crucial role in human immunity. Funding: Wellcome Trust, Academy of Medical Sciences starter grant
Switch Canonique en Cis ou Trans et Recombinaisons Suicides du Locus IgH by Iman Dalloul( )

1 edition published in 2018 in French and held by 1 WorldCat member library worldwide

Characterizing the antibody response at the single cell level with droplet microfluidics by Carlos Castrillon( )

1 edition published in 2018 in English and held by 1 WorldCat member library worldwide

Antibodies are Y shaped proteins, found as a component of circulating serum, that help the immune system target and respond to pathogens and foreign molecules, but can also contribute to disease when reacting to constitutive self-proteins. Antibodies are produced Plasma Cells, which secrete them into circulation. Because there's no physical link between Plasma Cells and their secreted antibodies, the detection of antigen-specific antibodies is problematic. In this thesis I explore the use of droplet microfluidics to generate and manipulate homogeneous aqueous compartments in which single antibody secreting cells can be isolated and analyzed in a high-throughput manner. To characterize single antibody secreting cells inside the droplets I use a novel assay that allows to interrogate cells based on the specificity of their secretion. These droplet compartments can be sorted for single cell antibody sequencing, or analyzed over time to obtain kinetic information of the antibody-antigen interaction inside each droplet. Using new established technology I was able to obtain the antibody repertoire of mice immunized against two different antigens from single antigen-specific antibody secreting cells, with equal or better capacities than current available technologies. Also, I was able to follow the affinity maturation process of antibodies at the single-cell level, both in immunization and autoimmune disease. With these tools I demonstrate how microfluidics can be used to characterize the immune and the autoimmune responses through the evaluation of single antibody secreting cells
Plasmocytes et désordres immunitaires : impacts des chimiokines et de leurs récepteurs sur la biologie des cellules sécrétrices d'anticorps dans le syndrome WHIM et le lupus systémique by Jessica Natt( )

1 edition published in 2017 in French and held by 1 WorldCat member library worldwide

The chemokines (CK) and their receptors (CKR) are essential for leukocyte homeostasis. They are also involved in the pathophysiology of several diseases including the WHIM syndrome and systemic lupus erythematosus (SLE).The WHIM syndrome is a rare immunodeficiency characterised by a severe peripheral lympho-neutropenia. It is caused by a gain-of-function of the CKR CXCR4, leading to a defect in desensitization of this receptor and a hyper-responsiveness to its ligand CXCL12. To better understand the pathophysiology of the WHIM syndrome, our laboratory developed the mouse model Cxcr4+/1013 harboring a natural mutation observed in some patients. Despite the lymphopenia, WHIM patients can mount a potent humoral immune response but that is not sustained over time. The maintenance of long-term antibody (Ab) titers is guaranteed by plasma cells (PCs) in the bone marrow (BM). The first part of my thesis was thus dedicated to the analysis of the role of a gain-of-function of Cxcr4 on PC differentiation and migration. The analysis of humoral response of Cxcr4+/1013 mice revealed a defect in the persistence of specific antibody titres in the absence of Cxcr4 desensitization. Furthermore, this was associated with an abnormal accumulation of a population of immature PCs in the BM.The second part of my work aimed to characterize the migratory potential of circulating PCs from SLE patients. SLE is a systemic autoimmune disease which can affect several organs like the skin, the kidneys or the central nervous system. SLE evolves in flare and remission phases and auto-Ab secreted by autoreactive PCs contribute to its pathophysiology and severity. Today, no specific treatment against autoreactive PCs exist. Targeting their migratory capacity to the inflamed tissues could be an alternative strategy. The objective of this project was to identify the migratory potential of SLE PCs. These studies were processed on samples from SLE patients during flare or remission, and on the lupus mouse model, the NZB/W F1 mice. We observed that the expression of different combinations of surface CKR may stratify several groups of SLE patients
La cytokine BAFF et les cellules T CD4+ sont des facteurs de survie majeurs pour les plasmocytes spléniques dans le contexte de déplétion B chez la souris : implications thérapeutiques pour les maladies auto-immunes by Lan-Huong Thai( )

1 edition published in 2016 in French and held by 1 WorldCat member library worldwide

Previous data suggested that the monoclonal anti-CD20 antibody induced paradoxically the settlement of autoreactive splenic long-lived plasma cells (LLPC) in the spleen of patients with auto-immune cytopenia, explaining the treatment failure. To investigate whether this process had a general relevance and decipher its mechanism, we used the AID-CreERT2-EYFP mouse model, which allows the irreversible expression of EYFP in B cells engaged in an immune response after tamoxifen regimen to follow plasma cells at different times after immunization. When analyzed by multiplex PCR at the single-cell level, while the splenic EYFP+B220-PC of untreated mice displayed an intermediate profile between short-lived and long-lived PC, the PC from anti-CD20 treated mice composed a more mature homogeneous population, similar to the long-lived bone marrow PC. The absolute number of splenic EYFP+B220-PC did not change significantly upon anti-CD20 treatment indicating that B-cell depletion promoted PC differentiation rather than a long-lived PC selection. BAFF (B-cell activating factor) and CD4+ T-cells played a major role in plasma cell survival since combination of anti-CD20 with anti-BAFF or anti-CD4 antibodies dramatically reduced the number of splenic EYFP+B220- LLPC. Anti-CD20 treatment also promoted the differentiation of LLPC in the spleen in the lupus prone NZB/W model, while a treatment combining anti-CD20 with anti-BAFF induced a marked reduction in total splenic PC numbers. These results suggest that the process of PC maturation upon anti-CD20 treatment is a general mechanism and that interfering with anti-BAFF antibody at the time of B-cell depletion might greatly improve the response rate in auto-immune disease
T follicular Helper Cells : Methods and Protocols by Marion Espéli( )

1 edition published in 2015 in English and held by 1 WorldCat member library worldwide

This volume brings together the skills and protocols of numerous laboratories that are at the heart of investigation into the biology of Tfh cells in both mice and humans. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Concise and easy-to-use, T follicular Helper Cells: Methods and Protocols provides scientist with techniques and protocols that have facilitated breakthroughs in Tfh biology and to present them in a way that will enable both new and experienced researchers to continue to move this exciting field forward
Analyse exploratoire des thérapies antisens innovantes dans le traitement des syndromes lymphoprolifératifs et autres maladies impliquant la lignée lymphoïde B by Anne Marchalot( )

1 edition published in 2020 in French and held by 1 WorldCat member library worldwide

Les oligonucléotides antisens (ASO) peuvent être utilisés pour diriger certaines étapes de la maturation des ARN comme l'épissage et la polyadénylation afin de moduler l'expression génique. Durant le développement des cellules de la lignée lymphoïde B, la maturation des transcrits d'immunoglobulines (Ig) fait intervenir des mécanismes d'épissage et de polyadénylation alternatifs. Mes travaux de thèse ont montré que les ASO peuvent être utilisés pour découpler les deux étapes interconnectées que sont l'épissage et la transcription des transcrits germinaux (GLT) des Ig afin d'étudier l'impact de l'épissage seul sur la commutation de classe d'Ig. Le dépôt du brevet n°EP19305716.3 m'a également permis de faire une preuve de concept dans l'utilisation des ASO pour influencer la polyadénylation d'un isotype spécifique d'Ig en ciblant les sites de polyadénylation (PAS) alternatifs responsables de la production d'Ig membranaire ou sécrétée. En effet, cibler le PAS utilisé pour le transcrit de l'IgE sécrétée avec un ASO a permis de réduire la sécrétion d'IgE et pourrait constituer une nouvelle piste thérapeutique dans les allergies IgE dépendantes. Enfin, j'ai développé une méthode de knock down par oligonucléotide modulant l'épissage (SSO) qui induit un saut d'exon lors de l'épissage et l'apparition d'un transcrit alternatif portant un décalage du cadre de lecture et un codon stop prématuré. Cette méthode permettrait de cibler de nombreux gènes exprimés dans les cellules eucaryotes comme la cellule B ou le plasmocyte.En dépit de leurs limitations en termes de biodistribution, les ASO constituent des pistes innovantes pour le développement de nouvelles thérapies contre les maladies impliquant la lignée lymphoïde B ou les lymphoproliférations
Nanoparticules de palmitate de dexaméthasone pour le ciblage passif dans le traitement de la polyarthrite rhumatoïde. by Mathilde Lorscheider( )

1 edition published in 2017 in French and held by 1 WorldCat member library worldwide

We developed nanoparticles of a glucocorticoid prodrug, dexamethasone palmitate (DXP) for the treatment of rheumatoid arthritis (RA). Joint inflammation, bone and cartilage erosion and dysregulation of the immune system characterize this autoimmune disease. Among the treatments indicated in RA, the use of glucocorticoids is hampered by their side effects induced by their unfavorable pharmacokinetics. To treat RA intravenously, the formulation of PEGylated nanoparticles seems essential in order to escape from opsonization and to obtain a specific accumulation in the joints inflamed. Therefore, we developed DXP nanoparticles (DXP-NPs) stabilized by the DSPE-PEG2000.The physicochemical characteristics of the nanoparticles obtained were evaluated as well as their stability over time. The amorphous internal structure of the nanoparticles and their very high drug loading thus prove the impact of DSPE-PEG2000 in the molecular organization of DXP-NPs. In vivo, the study of the pharmacokinetics and biodistribution of DXP-NPs following intravenous administration demonstrated prolonged circulation of the system. In a mouse model of rheumatoid arthritis, DXP-NPs their demonstrated specific accumulation in inflamed joints in correlation with significant therapeutic superiority in comparison with the water-soluble free molecule. Histological studies as well as adverse events evaluation supplemented the in vivo study
Molecular mechanisms regulating B lymphocyte polarization by Dorian Obino( )

1 edition published in 2016 in English and held by 1 WorldCat member library worldwide

In secondary lymphoid organs, B cells acquire antigens that are tethered at the surface of neighboring cells. Engagement of the B cell receptor (BCR) with such immobilized antigens leads to the formation of an immune synapse and the subsequent polarization of B cells. This includes the repositioning of the centrosome towards the immune synapse as well as the recruitment and local secretion of lysosomes required for efficient antigen extraction, processing and presentation onto class II major histocompatibility complex (MHC-II) molecules to primed CD4+ T cells. Pioneer work performed in the lab has highlighted the first molecular players involved in this process. However, the precise mechanism governing centrosome polarization remains to be fully elucidated. The work performed during this thesis aimed at identifying new regulators supporting centrosome polarization in B lymphocytes upon BCR engagement with immobilized antigens. In addition, in view of the emerging role played by the tissue microenvironment in shaping B cell activation and functions we investigated whether extracellular Galectin-8 modulates the ability of B cells to polarize, extract and present immobilized antigens. We show here that, in resting lymphocytes, centrosome-associated Arp2/3 (actin related protein-2/3) locally nucleates F-actin, which is needed for centrosome tethering to the nucleus via the LINC (linker of nucleoskeleton and cytoskeleton) complex. Upon lymphocyte activation, Arp2/3 is partially depleted from the centrosome as a result of its HS1-dependent recruitment to the immune synapse. This leads to a reduction in F-actin nucleation at the centrosome and thereby allows its detachment from the nucleus and polarization to the synapse. In addition, we show that extracellular Galectin-8 favors lysosome recruitment and secretion at the immune synapse, hence providing B cells with an enhanced capacity to extract and present immobilized antigens. Our findings highlight unexpected mechanisms that tune B cell polarity in response to antigenic stimulation and raise exciting questions concerning the coordinated regulation of these mechanisms to provide B cells with the capacity to efficiently extract, process and present surface-tethered antigens
Impact d'un gain de fonction de CXCR4 sur la différenciation et la biologie plasmocytaire by Nagham Alouche( )

1 edition published in 2018 in English and held by 1 WorldCat member library worldwide

Le récepteur CXCR4 et son ligand chimiokinique CXCL12 jouent un rôle essentiel dans le développement des cellules B et contribuent à la migration des plasmocytes (PCs) vers la moelle osseuse (MO) où ces cellules persistent dans des niches particulières et produisent des anticorps au long cours. Des mutations hétérozygotes du gène codant CXCR4 ont été identifiées dans un déficit immunitaire rare appelé le syndrome WHIM (SW) et récemment dans la macroglobulinémie de Waldenström (MW), un lymphoplasmacytome B caractérisé par l'expansion d'un clone IgM+ qui s'accumule dans la MO. Ces mutations affectent l'étape de désensibilisation du récepteur en réponse à CXCL12 et mènent donc à un gain de fonction. Mes travaux de thèse ont porté sur l'étude de l'impact du gain de fonction de Cxcr4 sur la biologie plasmocytaire par le moyen d'un unique modèle murin porteur de la mutation Cxcr4+/1013 précédemment identifié chez certains patients. Afin de mieux comprendre un défaut de vaccination présent chez les patients atteints du SW, la première partie de ma thèse s'est basé sur l'étude de la réponse humorale Thymo-dépendent du modèle murin Cxcr4+/1013. Nous avons mis en évidence un défaut de maintien de la réponse vaccinale probablement lié à l'absence des PCs spécifiques de l'antigène dans la MO des souris mutantes. En revanche, une population de plasmablastes (PBs) immatures dérivée de la réponse extra-folliculaire (EF), s'accumulent de façon aberrante suggérant une occupation des niches qui pourrait être à l'origine de la réponse vaccinale défectueuse. D'une autre part, dans la MW, les mutations CXCR4 sont toujours retrouvées en association avec des mutations gain de fonction du gène MYD88, la deuxième partie de ma thèse a donc porté sur l'étude de la réponse Myd88-dependante dans le modèle Cxcr4+/1013. Nos résultats révèlent une entrée en cycle accrue des cellules B Cxcr4+/1013 qui s'accompagne d'une augmentation de la différentiation plasmocytaire après activation de Myd88. De plus, nos résultats suggèrent, que le gain de fonction de Cxcr4 affecte la maturation des PBs ainsi que leur code migratoire ce qui pourrait expliquer leur migration exacerbée vers la moelle osseuse. L'ensemble de mes travaux a permis de mettre en évidence que la désensibilisation de Cxcr4 était un mécanisme clé régulant la réponse EF ainsi que la différentiation et la maturation plasmocytaire
Association Between Genetic Variation in FOXO3 and Reductions in Inflammation and Disease Activity in Inflammatory Polyarthritis( )

1 edition published in 2016 in English and held by 1 WorldCat member library worldwide

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1 edition published in 2016 in English and held by 1 WorldCat member library worldwide

Mécanismes moléculaires impliquant JAM-C dans le maintien du caractère souche des cellules de leucémies aigües myéloïdes by Julien Grenier( )

1 edition published in 2021 in French and held by 1 WorldCat member library worldwide

Acute myeloid leukaemia (AML) is a cancer resulting from blasts proliferation emerging from leukemic stem cells (LSCs). Recently, we have shown that JAM-C identifies a population of LSCs with leukemic initiation activity and exacerbated Src kinase activity. My thesis project consisted to uncover the signaling pathways downstream of JAM-C that contribute to leukemic initiation. Using a mouse model of AML, we were able to demonstrate that JAM-C is involved in early steps of leukemic initiation. Similarly, in Human AML, JAM-C expression is associated with stemness activity and the LSC marker GPR56. Surprisingly, we found that JAM-C could interact with the spliceosome through cleavage and release of its intracellular domain. This hypothesis was supported by the existence of a splicing signature correlated with JAM-C expression. This discovery allowed us to develop a prognostic score based on alternative splicing (SPL9) that predicts treatment response. We hope that in the near future this score can be used in clinical routine to help therapeutic decision and improve the management of AML patients
 
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T follicular helper cells : methods and protocols
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Bone marrow environment : methods and protocols
Alternative Names
Marion Espéli researcher

Marion Espéli wetenschapper

Languages
English (24)

French (10)