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United States Department of Energy Oakland Operations Office

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Works: 2,581 works in 2,883 publications in 1 language and 3,347 library holdings
Roles: Researcher
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Final Technical Report ( )
11 editions published between 1996 and 2005 in English and held by 16 WorldCat member libraries worldwide
Two primary technologies have been employed for analysis and measurement of the Synechocystis proteome. (1) 2D-gel electrophoresis. Currently one of the most reliable options in quantitative proteomics, typical 2D-gel experiments use isoelectric focusing (IEF) in the first dimension. In the case of membrane proteins, detergents must be added to maintain their solubility though only neutral/zwitterionic surfactants are compatible with the IEF process. We have optimized 2D gel separations for Synechocystis proteins extracted and separated into soluble and membrane subfractions. The resolution and coverage of integral membrane proteins is only marginally satisfactory and alternatives to the first dimension are being considered. Size-exclusion chromatography under non-denaturing conditions was one option that was explored but resolution was insufficient for subfractionation of the membrane-bound proteome. A more highly resolving technique, the ''Blue-native gel'' has proven excellent for Synechocystis and we plan to set up this technology in the near future. Proteins with altered expression are being identified through standard LCMSMS technologies. The analysis of PSI, PSII and SDH deficient mutants is completed, establishing the comparative aspect of the project for integration with the ultrastructural and metabolomic experiments at ASU. We are also looking forward to receiving ftsZ and VIPP1 interruption mutants to explore the effects on the proteome of cell enlargement and disruption of thylakoid biogenesis, respectively. (2) 2D liquid chromatography with mass spectrometry of intact proteins. Early experiments with total membrane protein extracts of Synechocystis showed that the spatial resolution of the reverse-phase separation used in front of the mass spectrometer limited detection to the one hundred or so most abundant proteins. The intact mass tags (IMTs) measured in this experiment represent the first of these measurements that will ultimately define the entire proteome. While some of the IMTs were matched to masses calculated from translations of genomic open-reading frames allowing reasonably confident identification of about half of them (hypothetical IMTs), we are currently validating identifications using a combination of peptide mass fingerprinting after cyanogen bromide cleavage and LC-MSMS after trypsin, of protein in fractions collected during LC-MS+. In order to gain more complete proteome coverage we are applying a liquid separation in front of the LC-MS+ experiment. Size-exclusion chromatography is the first separation technology to be employed, yielding immediate benefits, while still not satisfactory for overall resolution of complexes. Total membranes were solubilized with dodecyl maltoside (1.5%) and separated on deactivated silica (G 4000 SW). LC-MS+ analysis of less-retained chlorophyll-containing fractions, using reverse-phase and size-exclusion technologies, yielded intact protein mass spectra of the two large photosystem I subunits PsaA and PsaB as well as many other IMTs (Figures 1 & 2). These integral membrane proteins have eleven transmembrane helices and, at 81 and 83 kDa, represented one of the most significant challenges to the intact protein molecular weight approach. The identities of the proteins were confirmed by peptide mass fingerprinting and while there is good general agreement between measured and calculated masses it is noted that modest post-translational modifications are necessary to account for the measured molecular weights of the intact proteins. Whether these discrepancies are due to genuine post-translational modifications or DNA sequence errors remains to be determined. The data have been published allowing us to claim to be the first to have completed high-resolution electrospray-ionization mass spectrometry of the core subunits of Photosystem II, Photosystem I and the cytochrome b{sub 6}f complex providing effective proof-of-principle for application of the intact mass approach to the integral membrane proteome. Significantly, we reported greater integral membrane proteome coverage than a colleague studying thylakoids of Arabidopsis illustrating the benefits of the technique over sequential organic extraction of membrane proteins and 1D-gel analysis. The homogeneity of the PsaA and PsaB protein mass spectra attest to the quality of material grown at ASU and the viability of extraction and work up of the material after transport to UCLA
Progress report by Geoffrey M Wahl ( )
12 editions published between 1991 and 1993 in English and held by 12 WorldCat member libraries worldwide
This report discusses the following topics: Rayleigh-Benard convection with an imposed horizontal flow; Rayleigh-Benard convection in a gas under non-boussinesq conditions; electro- convection in a nematic liquid crystal; and Rayleigh-Benard convection in a nematic liquid crystal. (LSP)
Final Report ( )
5 editions published between 1998 and 2005 in English and held by 6 WorldCat member libraries worldwide
OAK-B135 This is the final report from the project Hydrodynamics by High-Energy-Density Plasma Flow and Hydrodynamics and Radiation Hydrodynamics with Astrophysical Applications. This project supported a group at the University of Michigan in the invention, design, performance, and analysis of experiments using high-energy-density research facilities. The experiments explored compressible nonlinear hydrodynamics, in particular at decelerating interfaces, and the radiation hydrodynamics of strong shock waves. It has application to supernovae, astrophysical jets, shock-cloud interactions, and radiative shock waves
High energy physics ( )
6 editions published between 1991 and 1997 in English and held by 6 WorldCat member libraries worldwide
This proposal is for the continuation of the High Energy Physics Program at the University of California, Riverside. In 1990, we will concentrate on analysis of LEP data from the OPAL detector. We expect to record 10⁵ Z's by the end of 1989 and 10⁶ in 1990. This data will be used to measure the number of quark-lepton families in the universe. In the second half of 1990 we will also be occupied with the installation of the D-Zero detector in the Tevatron Collider and the preparation of software for the 1991 run. A new initiative made possible by generous university support is a laboratory for detector development at UCR. The focus will be on silicon strip tracking detectors both for the D-Zero upgrade and for SSC physics. The theory program will pursue further various mass-generating radiative mechanisms for understanding small quark and lepton masses as well as some novel phenomenological aspects of supersymmetry
Energy transfer processes in solar energy conversion. Progress report ( )
6 editions published between 1986 and 1992 in English and held by 6 WorldCat member libraries worldwide
We have made substantial progress in experimental and theoretical studies in two areas: Photoinduced donor to acceptor electron transfer followed by back transfer in random solutions; and electronic excitation transport in systems with complex inhomogeneous spatial geometries and inhomogeneous energy distributions. Through the development of accurate statistical mechanical theories, we have been able to relate dynamics in complex systems to experimental observables. We have then used the experimental observables, time resolved fluorescence depolarization and transient grating experiments, to examine well defined molecular systems. The agreement between theory and experiment is excellent. 11 refs
Fundamental studies of fluid mechanics and stability in porous media. Progress report ( )
5 editions published between 1991 and 1993 in English and held by 5 WorldCat member libraries worldwide
This report summarizes accomplished and proposed work for the fundamental studies of fluid mechanics and stability in porous media. Topics discussed include: viscous fingering in miscible displacements; polymer flow interactions in free shear layers of viscoelastic fluids; effect of nonmonotonic viscosity profiles on the stability of miscible displacements in porous media; and references. (JL)
An Investigation of the Mechanism of IGA ( )
5 editions published between 1999 and 2000 in English and held by 5 WorldCat member libraries worldwide
OAK-B135 An Investigation of the Mechanism of IGA/SCC of Alloy 500 in Corrosion Accelerating Heated Crevice Environments. Technical progress report Note: This report was submitted electronically even though Part II A indicates by ''PAPER''
Salient issues of edge physics pertaining to loss of confinement a resistive MHD analysis. FY91 technical progress report ( )
5 editions published between 1990 and 1992 in English and held by 5 WorldCat member libraries worldwide
The progress that has been made during this fiscal year is significant in the area of tokamak edge plasma transport. The drift-rippling mode model of edge turbulent transport was extended. In particular, the research areas on which were concentrated include the following topics: (1) The theoretical investigation of the radiatively enhanced transport due to the effects of impurity driven radiation instabilities has been expanded to include a situation with multiple impurities (such as carbon, C{sup 4+}, and oxygen, O{sup 6+}); (2) In order to validate the use of the impurity radiation input from the tokamak bolometer experiments in the theoretical edge turbulent transport calculations, the analysis that is utilized to transform impurity brightness data to radiated power profiles has been checked for state population and Abel inversion correctness; (3) The drift-rippling model of edge turbulent transport has been extended to include ionization particle sources in addition to the impurity driven thermal instability drive; and (4) The detailed limiter and realistic edge geometric effects on the edge turbulent transport has been included in the drift-rippling model
Nuclear Energy Research Initiative (NERI) Program. Technical progress report ( )
5 editions published in 2000 in English and held by 5 WorldCat member libraries worldwide
During the past quartile the authors have explored the thermodynamics of various metal hydroxide and oxyhydroxide dehydration reactions of iron, nickel, and chromium with the objective of estimating the critical temperature, T{sub cr}. This is the temperature at which the oxide is in equilibrium with the oxyhydroxide or hydroxide and water vapor under the expected repository conditions of temperature and pressure. Because localized corrosion requires the presence of an ionically conducting phase on the surface (to support oxygen reduction) T{sub cr} is also the temperature below which ''wet'' corrosion first becomes possible. Past analyses have assumed that wet corrosion requires the presence of a layer of bulk water on the surface and hence that corrosion is not possible at temperatures above the boiling temperature of bulk water under the prevailing atmospheric pressure. Furthermore, significant elevation of the boiling temperature may occur if the aqueous phase becomes concentrated in salts. Because of the hydration of hygroscopic corrosion products, which may occur at significantly higher temperatures than the boiling temperature of water, and because of boiling temperature elevation effects, the past assumption needs to be reevaluated. This is the primary goal of this work
High resolution Raman spectroscopy of complexes and clusters in molecular beams. Performance report ( )
5 editions published between 1989 and 1991 in English and held by 5 WorldCat member libraries worldwide
The DOE-sponsored project in this laboratory has two facets. The first is the development of methods of nonlinear Raman spectroscopy for application in studies of sparse samples. The second is the application of such methods to structural and dynamical studies of species in supersonic molecular beams. The progress we have made in both of these areas is described in this paper. The report is divided into five remaining sections. The first pertains to theoretical and experimental developments in Fourier transform stimulated emission spectroscopy and Fourier transform hole-burning spectroscopy. The second deals with progress in the development of ionization-detected stimulated Raman spectroscopies (IDSRS). The third describes results from the application of IDSRS methods to studies of jet-cooled benzene clusters. The fourth describes IDSRS results from studies of hydrogen-bonded complexes containing phenols. The fifth relates to studies of carbazole-(Ar){sub n} clusters
Particle Pressures in Fluidized Beds. Final report ( )
4 editions published between 1992 and 1996 in English and held by 4 WorldCat member libraries worldwide
This project studies the particle pressure, which may be thought of as the force exerted by the particulate phase of a multiphase mixture, independently of that exerted by other phases. The project is divided into two parts, one concerning gas and the other liquid fluidized beds. Previous work on gas fluidized beds had suggested that the particle pressures are generated by bubbling action. Thus, for these gas fluidized bed studies, the particle pressure is measured around single bubbles generated in 2-D fluidized beds, using special probes developed especially for this purpose. Liquid beds are immune from bubbling and the particle pressures proved too small to measure directly. However, the major interest in particle pressures in liquid beds lies in their stabilizing effect that arises from the effective elasticity (the derivative of the particle pressure with respect to the void fraction): they impart to the bed. So rather than directly measure the particle pressure, we inferred the values of the elasticity from measurements of instability growth in liquid beds the inference was made by first developing a generic stability model (one with all the normally modeled coefficients left undetermined)and then working backwards to determine the unknown coefficients, including the elasticity
High-Pressure Thermodynamic Properties of f-electron Metals, Transition Metal Oxides, and Half-Metallic Magnets ( )
2 editions published between 2004 and 2005 in English and held by 4 WorldCat member libraries worldwide
This project involves research into the thermodynamic propertiesof f-electron metals, transition metal oxides, and half-metallicmagnets at high pressure. These materials are ones in which thechanging importance of electron-electron interactions asthe distance between atoms is variedcan tune the system through phase transitionsfrom localized to delocalized electrons, from screened to unscreened magnetic moments, and from normal metal to one in which only a single spinspecie can conduct. Three main thrusts are being pursued:(i) Mott transitions in transition metal oxides, (ii) magnetism in half-metallic compounds, and(iii) large volume-collapse transitions in f-band metals
Response of a tundra ecosystem to elevated atmospheric carbon dioxide and CO₂-induced climate change ( )
4 editions published between 1990 and 1993 in English and held by 4 WorldCat member libraries worldwide
Northern ecosystems contain up to 455 Gt of C in the soil active layer and upper permafrost. The soil carbon in these layers is equivalent to approximately 60% of the carbon currently in the atmosphere as CO₂. Much of this carbon is stored in the soil as dead organic matter. Its fate is subject to the net effects of global change on the plant and soil systems of northern ecosystems. The arctic alone contains about 60 Gt C, 90% of which is present in the soil active layer and upper permafrost. The arctic is assumed to have been a sink for CO₂ during the historic and recent geologic past. The arctic has the potential to be a very large, long-term source or sink of CO₂ with respect to the atmosphere. In situ experimental manipulations of atmospheric CO₂, indicated that there is little effect of elevated atmospheric CO₂ on leaf level photosynthesis or whole-ecosystem CO₂ flux over the course of weeks to years, respectively. However, there may be longer- term ecosystem responses to elevated CO₂ that could ultimately affect ecosystem CO₂ balance. In addition to atmospheric CO₂, climate may affect net ecosystem carbon balance. Recent results indicate that the arctic has become a source of CO₂ to the atmosphere. This change coincides with recent climatic variation in the arctic, and suggests a positive feedback of arctic ecosystems on atmospheric CO₂ and global change. The research proposed in this application has four principal aspects: (A) Long-term response of arctic plants and ecosystems to elevated atmospheric CO₂; (B) Circumpolar patterns of net ecosystem CO₂ flux; (C) In situ controls by temperature and moisture on net ecosystem CO₂ flux; (D) Scaling of CO₂ flux from plot, to landscape, to regional scales (In conjunction with research proposed for NSF support)
The metered delivery of solids into pressure a radically new machine concept ( )
4 editions published in 1992 in English and held by 4 WorldCat member libraries worldwide
This is the second report on the development of a machine concept for the continuous movement and accurately metered delivery of particulate solids (e.g., coal), wet or dry, into environments of ambient or differential fluid or mechanical pressure. It includes the first disclosure of continuous, direct delivery of US power plant coal into 26 psi gas pressure. The pump has only one moving part and is self-cleaning
Radiation effects and micromechanics of SiC ( )
4 editions published between 1991 and 1997 in English and held by 4 WorldCat member libraries worldwide
The basic displacement damage process in SiC has been fully explored, and the mechanisms identified. Major modifications have been made to the theory of damage dosimetry in Fusion, Fission and Ion Simulation studies of Sic. For the first time, calculations of displacements per atoms in SiC can be made in any irradiation environment. Applications to irradiations in fusion first wall neutron spectra (ARIES and PROMETHEUS) as well as in fission spectra (HIFIR and FFTF) are given. Nucleation of helium-filled cavities in SiC was studied, using concepts of stability theory to determine the size of the critical nucleus under continuous generation of helium and displacement damage. It is predicted that a bimodal distribution of cavity sizes is likely to occur in heavily irradiated SiC. A study of the chemical compatibility of SiC composite structures with fusion reactor coolants at high-temperatures was undertaken. It was shown that SiC itself is chemically very stable in helium coolants in the temperature range 500--1000°C. However, current fiber/matrix interfaces, such as C and BN are not. The fracture mechanics of high-temperature matrix cracks with bridging fibers is now in progress. A fundamentally unique approach to study the propagation and interaction of cracks in a composite was initiated. The main focus of our research during the following period will be : (1) Theory and experiments for the micro-mechanics of high-temperature failure; and (2) Analysis of radiation damage and microstructure evolution
Neutron Capture Experiments on Unstable Nuclei ( )
2 editions published between 2003 and 2005 in English and held by 4 WorldCat member libraries worldwide
The overall objective of this project is the measurement of neutron capture cross sections of importance to stewardship science and astrophysical modeling of nucleosynthesis, while at the same time helping to train the next generation of scientists with expertise relevant to U.S. national nuclear security missions and to stewardship science. A primary objective of this project is to study neutron capture cross sections for various stable and unstable isotopes that will contribute to the Science Based Stockpile Stewardship (SBSS) program by providing improved data for modeling and interpretation of nuclear device performance. Much of the information obtained will also be important in astrophysical modeling of nucleosynthesis. Measurements of these neutron capture cross sections are being conducted in collaboration with researchers at the Los Alamos Neutron Science Center (LANSCE) facility using the unique Detector for Advanced Neutron Capture Experiments (DANCE). In our early discussions with the DANCE group, decisions were made on the first cross sections to be measured and how our expertise in target preparation, radiochemical separations chemistry, and data analysis could best be applied. The initial emphasis of the project was on preparing suitable targets of both natural and separated stable europium isotopes in preparation for the ultimate goal of preparing a sufficiently large target of radioactive 155Eu (t1/2 = 4.7 years) and other radioactive and stable species for neutron cross-section measurements at DANCE. Our Annual Report, ''Neutron Capture Experiments on Unstable Nuclei'' by J. M. Schwantes, R. Sudowe, C. M. Folden III, H. Nitsche, and D. C. Hoffman, submitted to NNSA in December 2003, gives details about the initial considerations and scope of the project. During the current reporting period, electroplated targets of natural Eu together with valuable, stable, and isotopically pure 151Eu and 153Eu, and isotopically separated 154Sm were measured for the first time at the DANCE facility in early 2004. The Eu targets, suitable blanks, Be backing foils, and standards had been sent to the DANCE group in early fall 2003. Some preliminary data analysis was performed and more sophisticated analysis has begun. We developed plans for a suitable computer system for data analysis within our group at Berkeley and had meetings with counterparts at Lawrence Livermore National Laboratory (LLNL) and LANL concerning analysis of these data. Our major emphasis in 2004 has been to develop the separations and processes ultimately required to prepare radioactive targets of 4.7-year 155Eu. Efforts continued to devise an optimum multiprocess procedure suitable for use in separating radioactive 155Eu already produced by irradiation of stable 154Sm in a high neutron flux reactor at the Institut Laue-Langevin in France and shipped to LANL (the 22-min 155Sm neutron-capture product decays to 155Eu). This separation is extremely demanding because the highly radioactive 155Eu must be isolated from about 20 times as much mass of samarium before a target can be prepared for DANCE measurements. After all the procedures have been fully tested the radioactive 155Eu will be separated. The same electroplating methods already used successfully to prepare stable Eu isotope targets will be used to prepare the 155Eu target for DANCE. Discussions were held with LANL radiochemists in the Chemistry (C) Division about appropriate facilities at LANL for conducting the full-scale separation and purification of the radioactive targets. Three more multiprocess separations were developed that generated less chemical and radioactive waste, but they must still be adapted for processing hundred-milligram quantities. Until these separations can be successfully implemented at this scale, standard HPLC procedures will be used for separating and preparing radioactive 155Eu, 2.6-year 147Pm, and 1.9-year 171Tm target materials. Future directions beyond the preparation of radioactive lanthanide targets include closer collaboration with both LLNL and LANL to prepare actinide targets such as plutonium, americium, and curium. Also, the applicability of established and novel techniques will be evaluated for rapid separations of Am and Cm required in the irradiation of 241Am. Lastly, we will conduct a series of experiments aimed at enhancing current methods used to electrodeposit lanthanide and actinide targets on thin Ti and Be backings
Micro and Nano-structure Development and Multiscale Physics at Sliding Metal Interfaces ( )
2 editions published between 2005 and 2006 in English and held by 4 WorldCat member libraries worldwide
This final report describes research on the response of ductile materials to extreme loading conditions and high strain rates during impact combined with sliding friction. The work has involved a collaboration among two groups at Los Alamos National Laboratory and a tribology research grouup at The Ohio State University. The project involved experimental work and computer simulations at both laboratories and continuum mechanics analysis at OSU, supplemented by testing at AWE, Harwell, UK. Results demonstrated the importance of vorticity and mechanical mixing near the sliding interface in the development of nanocrystalline tribomaterial that is far from equilibrium. The work also revealed that strain rate sensitivity is an important materials property for determining the development of the velocity profile during sliding. As such, it is a property that is key to understanding the evolution of sliding behavior
A theoretical investigation of mode-locking phenomena in reversed field pinches ( )
2 editions published in 2004 in English and held by 4 WorldCat member libraries worldwide
OAK-B135 This paper investigates the formation and breakup of the ''slinky mode'' in an RFP using analytic techniques previously employed to examine mode locking phenomena in tokamaks. The slinky mode is a toroidally localized, coherent interference pattern in the magnetic field which co-rotates with the plasma at the reversal surface. This mode forms, as a result of the nonlinear coupling of multiple m = 1 core tearing modes, via a bifurcation which is similar to that by which toroidally coupled tearing modes lock together in a tokamak. The slinky mode breaks up via a second bifurcation which is similar to that by which toroidally coupled tearing modes in a tokamak unlock. However, the typical m = 1 mode amplitude below which slinky breakup is triggered is much smaller than that above which slinky formation occurs. Analytic expressions for the slinky formation and breakup thresholds are obtained in all regimes of physical interest. The locking of the slinky mode to a static error-field is also investigated analytically. Either the error-field arrests the rotation of the plasma at the reversal surface before the formation of the slinky mode, so that the mode subsequently forms as a non-rotating mode, or the slinky mode forms as a rotating mode and subsequently locks to the error-field. Analytic expressions for the locking and unlocking thresholds are obtained in all regimes of physical interest. The problems associated with a locked slinky mode can be alleviated by canceling out the accidentally produced error-field responsible for locking the slinky mode, using a deliberately created ''control'' error-field. Alternatively, the locking angle of the slinky mode can be swept toroidally by rotating the control field
Nuclear research with the electromagnetic probe. Progress report ( )
4 editions published between 1988 and 1994 in English and held by 4 WorldCat member libraries worldwide
The project discussed in this progress report focusses on understanding the many facets and scales of strongly interacting systems using the electromagnetic probe. On one hand we are investigating the spin properties of the nucleon (proton and neutron) through its fundamental constituents (quarks and gluons). On the other hand we are studying the properties of nucleons in nuclei and the few-body systems. The E142 and the newly approved E143 experiments planned at the Stanford Linear Accelerator Center are designed to provide an answer to the mystery of the missing spin of the proton, while the new letter of intent submitted to SLAC, will investigate the so called Color transparency effect related to the prediction of PQCD for the (e, e(prime)p) quasielastic process in nuclei. Our research involvement at the Continuous Electron Beam Accelerator Facility focusses in Hall A. From the technical point of view we are building the Coemption polarimeter for the Hall A beam line. This device should allow a precise measurement of the electron beam polarization for several approved experiments. From the physics aspect of the project we plan to perform the transverse/longitudinal separation of the nuclear response at high momentum transfer in the quasielastic region, the photodisintegration of deuterium with the measurement of the recoil polarization of the proton and the electromagnetic form factor of few body systems experiment
Neutron scatter studies of chromatin structures related to functions ( )
4 editions published between 1989 and 1992 in English and held by 4 WorldCat member libraries worldwide
Despite of setbacks in the lack of neutrons for the proposed We have made considerable progress in chromatin reconstitution with the VLR histone H1/H5 and in understanding the dynamics of nucleosomes. A ferromagnetic fluid was developed to align biological molecules for structural studies using small-angle-neutron-scattering. We have also identified and characterized an intrinsically bent DNA region flanking the RNA polymerase I binding site of the ribosomal RNA gene in Physarum Polycephalum. Finally projects in progress are in the areas of studying the interatctions of histone H4 amino-terminus peptide 1-23 and acetylated 1-23 peptide with DNA using thermal denaturation; study of GGAAT repeats found in human centromeres using high resolution Nuclear magnetic Resonance and nuclease sentivity assay; and the role of histones and other sperm specific proteins with sperm chromatin
 
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