WorldCat Identities

CASE WESTERN RESERVE UNIV CLEVELAND OH

Overview
Works: 141 works in 189 publications in 1 language and 203 library holdings
Classifications: HV873,
Publication Timeline
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Most widely held works by CASE WESTERN RESERVE UNIV CLEVELAND OH
Programmed Cell Death in Breast Cancer( )

6 editions published between 1995 and 1998 in English and held by 6 WorldCat member libraries worldwide

Programmed cell death, or apoptosis, is a genetically regulated process through which a cell is active in bringing about its own death for the sake of the entire organism. Apoptosis is the mechanism by which the mammary gland normally undergoes involution. Also, apoptosis is the mechanism by which many cancer chemotherapeutic agents induce the death of breast cancer cells. This work has focused on the role of intracellular calcium as a mediator of the apoptotic process in the MCF-7 and MDA-MB-468 breast cancer cell lines. The findings indicate that release of calcium from intracellular stores by the calcium-ATPase inhibitor, thapsigargin, induces apoptosis in these cell lines. However, the cells mount an endogenous stress response that delays and inhibits the cell death response, so that the breast cancer cell lines are much less susceptible to thapsigargin-induced apoptosis than lymphoid cell lines, an observation that parallels the differential susceptibility of breast cancer and lymphomas to chemotherapy-induced cell death in vitro. One component of this work still in progress is to measure how the stress responses, and well know apoptosis inhibitors like Bcl-2, regulate intracellular calcium fluxes that mediate apoptosis. Ultimately, this work will provide new insight into the role of calcium in the apoptotic process in breast cancer cells and how the response of cells to apoptotic signals is regulated by endogenous stress responses
Neural Networks for Real-Time Sensory Data Processing and Sensorimotor Control by Randall D Beer( Book )

5 editions published between 1990 and 1992 in English and held by 5 WorldCat member libraries worldwide

One of the critical problems that we faced in modeling the neural control of escape turns in the cockroach was a quantitative analysis of the leg movements that generate the turn. Because the turn is very rapid, often spanning no more than 20 msec, conventional motion analysis techniques could not be employed. For that reason, we acquired an NAC high speed video and computer motion analysis system. With these tools, we have not attained a very good description of the movements that occur at each joint of each leg during turns in response to wind from four different angles relative to the animals body. Figure 1 is a typical stick figure generated by the computer system showing four successive frames at 5 msec intervals. The pairs of legs shown are, from front to back, legs in the prothoracic (T1), mesothoracic (T2), and metathoracic segments. In all legs the coxa, femur, and tibia segments are shown. These segments make two critical joint angles: the coxal-femur (C-F) joint and the femoro-tibial (F-T) joint. Keywords: Robotics. (kr)
TIG3 - A Novel Inhibitor of Breast Cancer Cell Proliferation( )

4 editions published between 2000 and 2003 in English and held by 4 WorldCat member libraries worldwide

The mechanism of inhibition of cancer cell proliferation by vitamin A is poorly understood because many of the targets that mediate the retinoid-dependent growth suppression are not known. We have recently identified a novel retinoid-responsive gene target, TIG3, that we believe may be a key player in mediating the retinoid-dependent suppression of tumor cell proliferation. Understanding the mechanism of TIG3 action may provide insights that lead to innovative new anti-breast cancer therapies. Exploiting this potential requires that we understand how TIG3 inhibits cell proliferation - the major goal of this proposal. Specific Aim 1: Subcellular location plays a major role in determining function, and knowing location provides clues about function. Therefore, our first goal is to localize TIG3 in breast cancer cells. Specific Aim 2: Most proteins contain distinct functional domains, some responsible for localization and others for function. We hypothesize that TIG3 is divided into distinct functional domains that are important for growth suppression. We are testing this hypothesis. Specific Aim 3: The TIG3 amino acid sequence/structure reveals no obvious catalytic functional domains, suggesting that TIG3 acts by modulating the function of other proteins. A major goal of the study is to identify these targets. During the first year we have: 1) constructed a plasmid-based TIG3 expression systems and used it to express TIG3 in cells; 2) identified a perinuclear localization of TIG3 in cells; 3) demonstrated that the TIG3 carboxy-terminal hydrophobic domain guides appropriate subcellular localization; 4) shown that the TIG3 carboxy-terminal tail is required for optimal cell killing; and 5) constructed an adenovirus expression system that permits efficient TIG3 expression for biochemical studies
Regulation of A Kinase Cascade Involved in Cancer and Normal Cell Growth( )

4 editions published between 1995 and 1997 in English and held by 4 WorldCat member libraries worldwide

This award was used to train Dr. Minhong Yan during his Ph. D. research, and as such has been highly successful. Dr. Yan received his Ph. D. in May of 1997, and began a Post-Doctoral fellowship in July 1 1997. In experiments performed under this award, Dr. Yan first mapped sites of phosphorylation on the MEK1 protein kinase, and next identified the role of the MEKK1 protein kinase in signal transduction, placing it upstream of the SEK1 kinase and the Stress-Activated Protein Kinase. MEKK1 directly phosphorylates tSEK1, which in turn phosphorylates SAPK. Subsequent to accomplishment of this Aim, Dr. Yan has identified a novel activity of MEKK1, specifically a tyrosine kinase activity associated with the MEKK1 kinase (thought to be exclusively a serine/threonine kinase heretofore) as well as a cellular substrate of MEKK that becomes phosphorylated on tyrosine to undetermined ends
New Microlayer and Nanolayer Polymer Composites( )

4 editions published between 1998 and 2001 in English and held by 4 WorldCat member libraries worldwide

Created new microlayered and nanolayered polymer composites for various novel and unique applications, (1) Produced clear microlayered composites with improved ballistic performance (see attached, interim report 6/1/98 - 12/31/98). (2) Developed microlayered composites with highly anisotropic conductivity (see attached, interim report 6/1/98 - 12/31/98). (3) Polymer nanolayered systems of polypropylene were created having discoidal, meso-form morphologies. Also, high density polyethylene has been changed to a 'shesh-kabab' structure due to the fact that the nano-layer thickness is less than the radius of gyration of the polymer macromolecule (see attached, interim report (1/1/99 - 12/31/99). (4) High barrier, injection moldable systems have been produced by microlayering a polymer with good water barrier and a polymer with good oxygen barrier. Taking advantage of differences in melting points, layer integrity was maintained during injection molding in between the two melting points. This concept, which utilizes and maintains the large interfacial surface area achieved with microalyering, is broadly applicable to highly immiscible polymeric systems. (see attached, interim report 1/1/00 - 12/31/00). (5) One-dimensional photonic materials with applications to optical limiting and optical switching have been developed in conjunction with the Naval Research Laboratory. This was accomplished by synergistically combining nanolayer coextrusion methodology with dispersion of nonlinear dyes and careful control of nanolayer refractive indices. (see attached, interim report 1/1/00 - 12/31/00 and progress to date, 1/1/01 - 5/31/01)
Prostatic Fluid Cells( )

4 editions published between 2002 and 2005 in English and held by 4 WorldCat member libraries worldwide

Most research that requires long-term propagation of prostate cancer (PCA) cells is carried out with three cell lines: DU 145, PC-3, and LNCaP. All but one of these lines, LNCaP, fail to express prostate-specific antigen (PSA), androgen receptors, and/or any other prostate phenotype. Some prostatic fluids contain prostate cancer cells. The goals of this research were as follows: (1) to test the tumorigenicity of, and to develop transplantable xenografts from, PCA cells in prostatic fluid; (2) to develop methods for enhancing the tumorigenicity of small numbers of these PCA cells without deliberately altering their genes; (3) to test these methods for enhancement of tumorigenicity with prostatic fluid cells; and (4) to initiate clinical followup. As detailed in the body of the report, the aims of the research have been limited by exceptionally weak clinical collaboration that has become progressively weaker. The fluids that the author tested earlier were promising, but samples tested in the past year have been quite inadequate. Co-injection of lethally irradiated, growth-factor-producing cells was encouraging in some experiments with some tumors, but results were quite variable in repeated experiments. The inadequacies in fluid samples and the repeatability of experiments are detailed in this report. The author concludes that, prostate cancer cells from prostatic fluids of a significant proportion of patients with primary prostate cancers can survive for a few months in nude mice, and they do not grow rapidly enough to form transplantable xenografts with the technology that is currently available
Episome-Based Gene Therapy Strategy for Treatment of Human Breast Cancer( Book )

4 editions published between 1997 and 1999 in English and held by 4 WorldCat member libraries worldwide

We have focussed our efforts on investigating the mechanisms by which antisense to type I insulin-like growth factor receptor (IGF-IR) or antisense to transforming growth factor beta (TGFbeta) reduces tumorigenicity in human and murine breast cancer cells. MDA-MB-435S human breast cancer cells expressing antisense IGF-IR enhanced apoptosis in vitro, which could contribute to the reduced tumorigenicity observed in vivo. A dramatic reduction in tumorigenesis and an absence of lung metastasis was seen in 3 models of immune compromised mice (nude, scid, scid-beige) injected with antisense IGF-IR transfected MDA-MB-435S cells. All mice injected with parental or control transfected cells developed large tumors. However, only the scid beige animals consistently exhibited pulmonary metastases indicating that this animal is the most suitable for studying the metastatic behavior of these human breast cancer cells. We have established baseline parameters for IL-2, IL-4, IL-5 and interferon gamma in EMT6 murine breast cancer cells and in antisense IGF-IR and antisense TGFbeta transfectants. Spleen cells from syngeneic mice injected with EMT6 cells expressing antisense TGF-beta show increased cytotoxicity. The percentage of apoptotic cells from tumors excised from these animals is significantly reduced
The Nigrostriatal Dopamine System and Methamphetamine: Roles for Excitotoxicity and Environment, Metabolic and Oxidative Stress( )

3 editions published between 2000 and 2002 in English and held by 3 WorldCat member libraries worldwide

Degeneration of the nigrostriatal dopamine system is linked to the pathophysiology of Parkinson's disease. Similarly, the psychostimulant drug, methamphetamine also produces relatively selective damage to nigrostriatal dopamine neurons and is a widespread problem and drug of abuse throughout the U.S. However, the neurochemical underpinnings that mediate methamphetamine toxicity and Parkinson's disease are unknown. Several variables common to methamphetamine toxicity and Parkinson's disease, each of which may be important but alone are insufficient, may account for the neurodegeneration of the nigrostriatal dopamine path. It is hypothesized that the convergence of excitotoxicity, free radicals and a depleted bioenergetic state produces damage to dopamine neurons. Moreover, environmental stressors, which also increase free radicals and excitatory amino acids predispose dopamine neurons to damage. Consequently, environmental stress may be synergistic with oxidative and metabolic insults as well as glutamate to culminate in dopamine cell death. The major objective is to examine the interaction between environmental stress and methamphetamine and the convergent action of excitotoxicity and bioenergetic and oxidative stress to produce damage to nigrostriatal dopamine neurons. A multidisciplinary approach will be used as well as pharmacological strategies that we posit to be neuroprotective against methamphetamine, excitotoxicity, and bioenergetic and oxidative stress will be examined
Investigating the Role of Nuclear Clustering (nCLU) in Lethality and Genomic Instability in Paclitaxel (taxol) - Treated Human Breast Cancer Cells( )

3 editions published between 2002 and 2004 in English and held by 3 WorldCat member libraries worldwide

Clusterin is a protein that has been implicated in many normal physiological processes (tissue remodeling, sperm maturation) as well as many pathological processes (Alzheimer disease, atherosclerosis, cancer) . Our laboratory became interested in clusterin when we identified it as an x-ray induced protein/transcript in human melanoma cells. Recently, we have identified two forms of this protein that are derived by alternative splicing of a single message. The secretory form of clusterin (sCLU) has been shown to have cytoprotective effects after cellular stress and injury. In contrast, a nuclear form of clusterin (nCLU) appears to be cytotoxic. Recently, Redondo et. al demonstrated that sCLU was overexpressed in breast cancer. sCLU over expression may provide a selective advantage in malignant cells. The most effective therapies for breast cancer after surgery include chemo- and radiation therapies. These therapies often fail as the tumor develop drug and radiation resistance. Our lab has shown that sCLU is induced by physiological doses of taxol, taxotere and radiation. Understanding the cellular and molecular responses of malignant and normal cells to these chemo- and radiation therapy would allow us to increase the efficacy of these treatments. Insight into the regulation of sCLU will allow us to better understand some of these processes
A Novel Apoptotic Protease Activated in Human Breast Cancer Cells After Poisoning Topoisomerase I( )

3 editions published between 1999 and 2001 in English and held by 3 WorldCat member libraries worldwide

The goal of this grant is to clone the unknown protease activated by the active anti-breast cancer agent, Beta-lapachone (Beta-lap). The research team showed for the first time that Beta-lap requires NQ01, a two-electron reduction enzyme elevated in many human breast cancers, for activation. The team then characterized the unknown apoptotic protease activated in human breast cancer cells by Beta-lap, defining endpoints which will be essential for the ultimate isolation of this novel apoptotic protease. The unknown protease: (a) is a non-caspase cytsteine protease; (b) cleaves p53, lamin B, and PARP (atypically) in an NQ01-dependent manner at a time co-incident with calpain activation (appearance of an 18 kDa active form and its movement into the nucleus by confocal microscopy); and (c) is calcium-dependent (e.g., the proteolytic cleavage of PARP or p53 was blocked by co-administration of EGTA or EDTA), and the drug causes massive NQ01-dependent calcium influx within 3 mins posttreatment with 5-8 micro B-lap
Statistical Mechanical Study of Polyvinylidene Fluoride( Book )

2 editions published between 1984 and 1987 in English and held by 2 WorldCat member libraries worldwide

A final report is presented of work performed in the study of the properties of polyvinylidene fluoride by the theoretical techniques of statistical mechanics and condensed-matter theory. The nature of piezoelectricity and pyroelectricity in polymers and their dependence on crystal structure and internal electric fields has been thoroughly investigated. The time-dependence and microscopic origin of the poling process has been studied and accounted for
Teacher Expectancy or My Fair Lady by Elyse S Fleming( Book )

1 edition published in 1970 in English and held by 2 WorldCat member libraries worldwide

This study examined the effects of teacher expectancy on changes in intelligence, known as the phenomenon of the self-fulfilling prophecy. Differences in sex, socioeconomic status, and teacher opinion about standardized tests were control factors. The teachers completed a questionnaire that provided the basis for classifying teachers into high, middle, and low opinion groups with regard to the value they placed upon the usefulness of intelligence tests. A total of 859 second grade students in 39 classes, with high and low poverty backgrounds, were randomly assigned to one of four treatment conditions. Teachers were provided with one of four kinds of test information for each group: (1) Kuhlmann-Anderson iq reported as tested, (2) IQs inflated by 16 points, (3) Primary Mental Abilities percentiles, and (4) intelligence test information withheld entirely. Following re-testing several months later, teachers were asked to assess iq accuracy for each child. No significant iq differences were found among the four treatment groups or between sexes. There were significant differences between teacher opinion, socioeconomic status, and for the interaction of teacher opinion and socioeconomic status. The study failed to support a generalized self-fulfilling prophecy. (Author/DR)
[Liberal arts/mathematics based alternative core, Case Western Reserve University] by John C Angus( Book )

1 edition published in 1989 in English and held by 2 WorldCat member libraries worldwide

The report describes development of an alternative core curriculum of courses grounded equally in both mathematics/sciences and traditional humanities. The alternative core curriculum is based on the belief that mathematics, broadly defined, is the common language and basis for the contemporary revolutions in the physical sciences, social sciences, technology, and information sciences. The program serves students majoring in the following fields: anthropology, computer science, economics, history, philosophy, political science, psychology, and sociology. Currently an average of 33 undergraduates per semester elect the alternative courses. The program has involved development of specific core courses, development of courses in the major field programs based on the alternative core, and preliminary development of entirely new Bachelors programs based on the alternative core. Eight Bachelor of Arts degree programs based on the alternative core are now in place. Recommendations include adding two more Bachelors programs to maintain a critical mass of students and ensure the long term viability of the alternative curriculum. Separate brief reports by faculty participants in each of the new courses are included. (DB)
Community, Creativity, Collaboration: A New Training Model in Mental Health. a Summary Report on the Comprehensive Community Mental Health Training Project by Cleveland, OH Case Western Reserve Univ.( Book )

2 editions published in 1970 in English and held by 2 WorldCat member libraries worldwide

This report presents an experiment in post-graduate training of mental health professionals through innovative education designed not only to inform but to produce changes in individual attitudes, roles, and skills, as well as in agency programs. The educational design was based on a human factors or sociocentric change model. Included in Part One is a summary of project activities with specifics on the goal, the educational model, the participants, the outcomes, and the evaluation of the training project. Major impacts are noted on individuals, agencies, and on relationships between agencies. Based on material from rating scales and interviews, there was in increased acceptance of the goals of community mental health, an expanded definition of the field, and a greater sense of professional responsibility. Part Two of the report provides a detailed description of the curriculum and program, including lists of staff and participants. The appendix provides a followup questionnaire and reference material. (Author/KJ)
Multi-Disciplinary Ph. D. Research Training. Final Progress Report by Cleveland, Oh Case Western Reserve Univ.( Book )

1 edition published in 1973 in English and held by 2 WorldCat member libraries worldwide

An analysis of the Graduate Educational Research Fellowship Training Program at Case Western Reserve University is presented. The key elements of the program involve: financial support of graduate sutdents from a number of social and behavioral science disciplines related to educational problems; commitment on the part of several departments in the social and behavioral sciences that they had a stake as well as a responsibility to be directly involved in the training of students who would eventually claim a professional interest in education; a detailed curriculum format that balanced a structured set of courses along with built-in opportunities for independent learning; and program coursework that clearly reflected the multidisciplinary character of the objectives of the program as well as the operational steps necessary to achieve these expressed goals. Changes to the program and strengths and difficulties of the program are discussed. (Spg)
Fair play : a report of a study of the administration of Aid to Families with Dependent Children in several midwestern states by Marie Vesely( Book )

2 editions published in 1982 in English and held by 2 WorldCat member libraries worldwide

Applying for public assistance can be a difficult, long, and humiliating process. Administrative practices can prevent applicants from receiving their lawful benefits from agencies handling Aid to Families with Dependent Children (afdc). To examine these problems, Project Fair Play recruited graduate social work and law students as advocates for afdc recipients; and gathered study data using a Case Report Form for each family seeking assistance in resolving an afdc problem. Agency and legal services questionnaires were developed for face-to-face or telephone interviews with social, legal, and human services agency staff, as well as a Welfare Client Survey for people in local welfare department waiting rooms. Analyses of the data collected showed nine major categories of findings including: (1) welfare system management problems; (2) barriers to entering the welfare system; (3) communication problems; (4) welfare system inefficiency; (5) welfare system insensitivity; (6) client characteristics; (7) child support problems; (8) detection of fraud problems; and (9) emergency service problems. Section 2 is a detailed discussion of each of the findings. A reference list and two appendices providing information on Project Fair Play student participants, clients and the afdc population are also included. (Pas)
Technology Transfer University Opportunities and Responsibilities. a Report on the Proceedings of a National Conference on the Management of University Technology Resources by Cleveland, Oh Case Western Reserve Univ.( Book )

1 edition published in 1974 in English and held by 2 WorldCat member libraries worldwide

The problem of transferring university "technology" to industry must be addressed in highly specific terms if satisfactory and conclusive results are to be expected. It is the general purpose of this conference report to identify major obstacles and to explore successful means for reducing or eliminating them. The conference was planned with the following objectives in mind: (1) to promote an increased interest among university administrators in the technology potential of their respective institutions; (2) to develop a more acute awareness of the need for more effective management of university technology resources; (3) to assemble university representatives with considerable experiences and expertise in the management of faculty discoveries and inventions to encourage them to communicate with one another, and to a broad university audience details relating to their policies, procedures, methodology, etc.; and (4) through an effective program format to maximize effective communication among all institutional representatives relating to pertinent problems now impeding the flow of "technology" into the market place. (Author/KE)
A Probabilistic Model of Brittle Crack Formation( Book )

2 editions published between 1987 and 1988 in English and held by 2 WorldCat member libraries worldwide

Probability of a brittle crack formation in an elastic solid with fluctuating strength is considered. A set omega of all possible crack trajectories reflecting the fluctuation of the strength field is introduced. The probability P(X) that crack penetration depth exceeds X is expressed as a functional integral over omega of a conditional probability of the same event taking place along a particular path. Various techniques are considered to evaluate the integral. Under rather non-restrictive assumptions we reduce the integral to solving a diffusion type equation. Here a new characteristic of fracture process, 'crack diffusion coefficient', is introduced. Then an illustrative example is considered where the integration is reduced to solving an ordinary differential equation
Crystal Structure of an Alcohol Dehydrogenase from the Extreme Thermophile Thermoanaerobium brockii( Book )

2 editions published in 1998 in English and held by 2 WorldCat member libraries worldwide

The crystal structure of a thermophilic alcohol dehydrogenase (TBAD) has been determined in complex with sec-butanol as substrate to a resolution of 3.0 A. The enzyme is from Thermoanaerobacter brockii, a bacterium isolated from hot springs in Yellowstone National Park. This tetrameric enzyme has a melting temperature of 98 C. The structure consists of two domains, the catalytic domain and the cofactor-binding domain, with the active site located in a deep cleft at the domain interface. Factors important for the thermostability have been deduced by comparison with the crystal structure of a highly homologous mesophilic alcohol dehydrogenase from Clostridium beijerinckii (CBAD). The thermophilic enzyme has a more hydrophilic exterior, a more hydrophobic interior. a smaller surface area, more prolines, alanines, and less serines than CBAD. Furthermore, in the thermophilic enzyme the number of all types of intersubunit interactions is increased: more salt bridges, hydrogen bonds and hydrophobic interactions. All these effects combined can account for the 32 deg C higher melting temperature of the thermophilic enzyme
Multimodality CT/SPECT Evaluation of Micelle Drug Carriers for Treatment of Breast Tumors( )

2 editions published between 2006 and 2007 in English and held by 2 WorldCat member libraries worldwide

Polymer micelles are a nanoparticle drug delivery system that has the potential to improve breast tumor treatment with chemotherapy. These nanoparticles can increase the half-life of incorporated drugs, can target tumors by incorporating tumor specific ligands, and can be tracked with imaging through the inclusion of a radiolabel. In this study, PEG/PLA or PEG/PCL micelles were modified through the addition of a cRGD targeting ligand and a DOTA chelating molecule for tracking with 111In SPECT imaging. In vitro, cRGD-targeted micelles were incorporated into cells at a faster rate than non-targeted micelles and exhibited cytotoxicity rivaling that of free doxorubicin. Furthermore, SPECT imaging of 111In-labeled micelles administered intravenously to tumor-bearing mice in vivo established the potential for micelle tracking with 111In and confirmed the prolonged distribution half-life of micelles. Future research will focus on further development of this micelle platform with the ultimate goal of simultaneously tracking micelle localization and monitoring antitumor efficacy. Fluorescent tracking of micelles through the incorporation of quantum dots and monitoring antitumor efficacy with fluorescently labeled Annexin V may provide an alternate and superior method of achieving this goal
 
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