WorldCat Identities

Lima Filho, Jose Luiz de

Works: 11 works in 11 publications in 2 languages and 10 library holdings
Genres: Academic theses 
Roles: Other, Author
Publication Timeline
Most widely held works by Jose Luiz de Lima Filho
A Screening Study of Potential Carcinogen Biomarkers After Surgical Treatment of Obesity by Luciana Teixeira de Siqueira( )

1 edition published in 2018 in English and held by 2 WorldCat member libraries worldwide

Protoplast formation and regeneration from Streptomyces clavuligerus NRRL 3585 and clavulanic acid production by Maria das Gracas Carneiro-da-Cunha( )

1 edition published in 2002 in English and held by 1 WorldCat member library worldwide

Protoplasts of the wild type Streptomyces clavuligerus NRRL 3585 (ATCC 27064) were formed from spores cultures obtained in the lag, exponential and stationary growth phases by using 0.5% glycine in the culture medium. The protoplasts were obtained by treatment of the cells with lysozyme (EC- 40,000 U (1mg/mL), in an osmotic solution for 90 min at 28o.C. The frequency of regenerated protoplasts in the lag phase was 1.7x10³ CFU/mL (28.97%), in the beginning of the exponential phase 0.4x10² CFU/mL (31.67%), in the exponential growth phase 2.5x10³ CFU/mL (46.30%) and 1.0x10(5) CFU/mL in stationary phase (48.45%). Antibiotic production and activity of regenerated protoplasts were observed in all phases, except in the lag phase. The protoplast formation and regeneration techniques resulted in a new isolate strain of Streptomyces clavuligerus that produced approximately 2.5 fold more clavulanic acid
Partial characterization of proteases from Spretomyces clavuligerus using an inexpensive medium by Keila Aparecida Moreira( )

1 edition published in 2001 in English and held by 1 WorldCat member library worldwide

The partial characterization of extracellular proteases from Streptomyces clavuligerus NRRL 3585 and 644 mutant was investigated. The enzyme production was carried out in batch fermentation using soy bean filtrate as nitrogen source. Maximum activity was obtained after 96h of fermentation with an initial pH of 7.0. The enzyme was partially purified by ammonium sulphate precipitation. Enzymes from the two strains retained 37 per cent of their initial activities at pH 8.0 after 2 h incubation at 25[vbar]C. Enzyme half-life at pH 8.0 and 60[vbar]C was 40.30 and 53.32 min, respectively for both strains (partially purified extract). The optimum pH was obtained at pH 7.0-8.0 and 8.4 for enzymes produced for 3585 and 644 strains (crude extract), respectively, and 8.4 and 8.0 for enzymes from the partially purified extract 3585 and 644 strains, respectively. The optimum temperature for the crude extract was 21[vbar]C for both strains. However, for the partially preparation the optimum temperature was 50[vbar]C and 40[vbar]C for S. clavuligerus NRRL 3585 and 644 strains respectively. (Au)
Production of extracellular lipase by the phytopathogenic fungus Fusarium solani FS1 by Maria de Mascena Diniz Maia( )

1 edition published in 1999 in English and held by 1 WorldCat member library worldwide

A Brazilian strain of "Fusarium solani" was tested for extracellular lipase production in peptone-olive oil medium. The fungus produced 10,500 U.L(E-1) of lipase after 72 hours of cultivation at 25[vbar]C in shake-flask at 120rpm in a medium containing 3(per cent) (w/v) peptone plus 0.5(per cent)(v/v) olive oil. Glucose (1(per cent) w/v) was found to inhibit the inductive effect of olive oil. Peptone concentrations below 3(per cent)(w/v) resulted in a reduced lipase production while increased olive oil concentration (above o.5(per cent)) did not further stimulate lipase production. The optimum lipase activity was achieved at pH 8.6 and 30[vbar]C and a good enzyme stability (80(per cent) activity retention) was observed at pH ranging from 7.6 to 8.6, and the activity rapidly dropped at temperatures above 50[vbar]C. Lipase activity was stimulated by addition of n-hexane to the culture medium supernatatnts, in contrast to incubation with water-soluble solvents (AU)
Genotipo MLB2 materno influencia a transmissao vertical em criancas brasileiras by Sergio Crovella( )

1 edition published in 2004 in Portuguese and held by 1 WorldCat member library worldwide

O presente estudo analisa os polimorfismos geneticos no primeiro exon MBL2, em um coorte de 21 pares de maes e filhos infectados por HIV-1 e um coorte de 11 pares constituidos por maes infectadas por HIV-1 e filhos nao infectados, na cidade de Recife(PE), localizada no nordeste do Brasil, com objetivo de avaliar se o genotipo MBL2, de maes infectadas por HIV-1 poderia estar relacionado com transmissibilidade do virus para seus conceptos. Todas criancas nasceram de maes infectadas por HIV-1 que nao haviam recebido nenhum tratamento antiretroviral durante a gravidez e nao foram submetidas a parto cesareano para prevenir transmissao. Maes que transmitiram HIV-1 a seus conceptos (MT) tinham frequencia mais alta (14 porcento) de genotipo 00 no MBL2(tipico de maus produtores de MBL) se comparado com frequencia de 0 porcento observada entre maes infectadas que transmitiram o virus para seus filhos (MNT). Esta diferenca foi estatisticamente significante. A presenca do genotipo 0 e 00 no alelo MBL2 em MT se associou com transmissao viral. O genotipo AA de MBL (associado com producao normal de MBL) teve frequencia de 82 porcento em MNT, significantemente mais alta que a frequencia de 52 porcento de MT. A presenca do alelo A em 91 porcento das MNT conferiu menor risco de transmissao vertical no coorte estudado. Nossos resultados sugerem que a transmissao vertical de HIV-1 depende somente do genotipo MBL2 da crianca e da mae, nas quais os niveis da proteina MBL poderiam desempenhar um importante papel na transmissao do virus
Immobilization of lipase from Fusarium solani FS1 by Kirsty Knight( )

1 edition published in 2000 in English and held by 1 WorldCat member library worldwide

Lipase from "Fusarium solani" FS1 was immobilized by covalent attachment to polyacrylamide beads and onto magnetized Dacron, retaining 12(per cent) and 97(per cent) of activity, respectively. Lipase was also entrapped within polyacrylamide beads, retaining 53(per cent) of activity. Investigations of the kinetic characteristics of the immobilized derivates using triolein as substrate showed that lipase immobilized onto polyacrilamide beads and Dracon did not follow Michaelis-Menten kinetics. (AU)
Partial characterization of proteases from streptomyces clavuligerus using an inexpensive medium by Keila Aparecida Moreira( )

1 edition published in 2001 in English and held by 1 WorldCat member library worldwide

A caracterizacao parcial de proteases extracelulares de Streptomyces clavuligerus foi investigada. A producao da enzima foi realizada pela fermentacao em batelada utilizando farinha de soja como fonte de nitrogenio. O maximo rendimento foi obtido apos 96 horas de fermentacao com um pH inicial de 7,0. A enzima foi purificada parcialmente por precipitacao com sulfato de amonia. Ambas enzimas reteram 37% de sua atividade inicial a pH 8,0, apos 2 horas de incubacao a 25[vbar]C. O tempo de meia-vida das enzimas foi de 40,30 e 53,32 minutos, respectivamente para ambos 3585 e 644 (extrato parcialmente purificado) a pH 8,0 e 60[vbar]C. Com relacao ao pH, a atividade otima foi alcancada a pH 7,0-8,0 e 8,4 para enzimas produzidas pelas amostras 3585 e 644 (extrato bruto) respectivamente e 8,4 e 8,0 para as enzimas obtidas a partir dos extratos parcialmente purificados das amostras 3585 e 644, respectivamente. A temperatura otima para o extrato bruto foi de 21[vbar]C para S. clavuligerus NRRL 3585 e 644. Contudo, para a preparacao parcialmente purificada, a temperatura otima foi de 50 e 40[vbar]C para amostras S. clavuligerus NRRL 3585 e 644 respectivamente. (AU)
Large scale purification of Clostridium perfringens toxins: a review by Maria Taciana Holanda Cavalcanti( )

1 edition published in 2004 in English and held by 1 WorldCat member library worldwide

Clostridium perfringens, a Gram-positive anaerobic bacterium, is widespread in the environment and commonly found in the intestines of animals, including humans. C. perfringens strains are classified into five toxinotypes (A, B, C, D and E) based on the production of four major toxins (alpha, ̄, epsilon, iota). However the toxins (theta, delta, lambda and enterotoxin) are also synthesized by C. perfringens strain. Many attempts to purify the toxins produced by C. perfringens have been proposed. In this review we discuss the purification methods used to isolate toxins from C. perfringens reported in last four decades
The development and evaluation of a microprocessor-controlled bioreactor for use in developing countries by Jose Luiz De Lima Filho( Book )

1 edition published in 1987 in English and held by 1 WorldCat member library worldwide

Production and Stability of Protease from Candida buinensis by Daniela de Araújo Viana( )

1 edition published in 2010 in English and held by 0 WorldCat member libraries worldwide

Detection of Human Papillomavirus in biopsies of patients with cervical cancer, and its association with prognosis by Carla Limeira Barreto( )

1 edition published in 2013 in English and held by 0 WorldCat member libraries worldwide

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