WorldCat Identities

McNaughton, Donald

Overview
Works: 9 works in 11 publications in 1 language and 16 library holdings
Genres: Parodies, imitations, etc  Academic theses 
Roles: Author
Classifications: PR6025.A2855,
Publication Timeline
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Most widely held works about Donald McNaughton
 
Most widely held works by Donald McNaughton
Ghosting for McGonagall by Donald McNaughton( Book )

2 editions published in 1945 in English and held by 7 WorldCat member libraries worldwide

Studies in rotational spectroscopy and structure of short-lived molecules by Donald McNaughton( Book )

2 editions published in 1981 in English and held by 2 WorldCat member libraries worldwide

Infrared spectroscopy of cold molecules by Christopher Michael Medcraft( )

1 edition published in 2012 in English and held by 1 WorldCat member library worldwide

A long path collisional cooling cell has been installed at the Far-Infrared beamline at the Australian Synchrotron to study the infrared spectrum of molecules at cryogenic temperatures. New transfer optics and vacuum chambers were designed in order to allow study in the far infrared region where the synchrotron source provides the most advantage. Experiments using this cooling cell can be divided into two themes: rotationally resolved high resolution spectroscopy and low resolution spectroscopy of aerosols (solid or liquid particles suspended in a gas). Chapter 1 contains the background information and theory important to high resolution spectroscopy. This chapter also contains details of the instrumentation used and the modifications to the optics of the cooling cell. Chapter 2.1 is the first publication, a report on the performance of the Far-IR beamline and the optical modifications to the cooling cell. This paper also contains examples of the data obtained with the new arrangement. The far-IR spectrum of carbon dioxide aerosols and the high resolution spectrum of 1,1-difluoroethane recorded at room temperature and at 154 K, showing the advantages of cooling for high resolution FTIR spectroscopy. Prior to the completion of the optical modifications the mid-IR spectrum of carbon-13 enriched C2F4 was measured in the cooling cell at 150 K. Chapter 2.2 contains the structural, vibrational and rovibrational analysis of this molecule. The ground state rotational constants for 12C13CF4 and 13C2F4 were determined and used to compute a semi-experimental equilibrium geometry. Upper state rotational constants were also determined. Chapter 2.3 contains a publication where the pure rotational spectrum of the important interstellar molecule oxirane (cyclic-C2H4O) was measured between 15 and 73 cm-1 (0.4-2.2 THz)
Hearing-Waiver Program : preliminary report by Donald McNaughton( Book )

1 edition published in 1969 in English and held by 1 WorldCat member library worldwide

Far-infrared ro-vibrational spectroscopy of coriolis coupled molecules of interstellar importance by Michael Kenneth Bane( )

1 edition published in 2012 in English and held by 1 WorldCat member library worldwide

One of the fundamental limiting factors in ro-vibrational spectroscopy has been the lack of high brilliance sources in the far-infrared region of the electromagnetic spectrum. Synchrotron sources are somewhat able to fill this gap, and spectroscopy of the usually weak lowest energy vibrational modes is becoming more accessible. A detailed knowledge of the molecular parameters of low energy vibrational modes is important for interstellar detection, since it is these modes that are most likely to be thermally populated in these systems. The first chapter of this thesis outlines the fundamental theory associated with the high- resolution vibration-rotation spectroscopy of molecules. Firstly, a theory of the allowable energies of molecules is presented, with careful consideration of the quantum mechanical nature of the entity. Secondly, a theory of the interaction with light is discussed, which relates to the observable absorbance and selection rules. Finally, the theory of the Fourier transform is discussed in relation to the collection and attributes of the recorded spectra. The second chapter discusses the instrumentation and analytical basics of the project. Detailed aspects of the synchrotron source and spectrometer are first outlined and give insight into the experimental work done during this project. For context, a discussion of complementary high-resolution techniques is included regarding the recording of both pure rotational and ro-vibrational spectra. The majority of this project involved detailedspectral analysis, and thus an explanation of the most useful tools and techniques is included. Finally a section justifying the selection of ketenimine, 1-phosphapropyne, thiirane and chlorodifluoromethane as target molecules is included. This also serves as a summary of the work done on each molecule, and elaborates slightly on what is presented in the publications. Chapter 3 contains the work published during this project. This work mainly relates to the analysis of high-resolution far-infrared spectra of molecules which are either predicted to be, or have been detected as, a part of the interstellar medium. Chapter 4 discusses the implications of this research, and suggests possible future extension. A major theme of this project is the de-perturbation of modes which exhibit strong coupling mechanics, highlighted by the characterization of an intensity stealing mechanism which allows effectively infrared inactive modes to be enhanced and become observable. This effect was found, by chance, to be crucial in explaining the spectra of two of the three molecules studied in this thesis. This leads to the tantalizing prospect that perhaps this mechanism may be important in the study of the ro-vibrational spectra of other molecules
Investigating biochemical and structural changes in animal models of multiple sclerosis using Fourier transform infrared imaging and small angle x-ray scattering by Sally Caine( )

1 edition published in 2012 in English and held by 1 WorldCat member library worldwide

Multiple sclerosis (MS) is a debilitating disease of the central nervous system and is the leading cause of non-traumatic neurological disability in young adults. MS affects over 2 million people worldwide and is commonly believed to arise from an autoimmune attack directed against components of the myelin sheath, resulting in multifocal lesions characterised by inflammation, demyelination and axonal damage. A complex interplay between genetic and environmental factors is thought to contribute to disease susceptibility. Although conventional histopathological assessment and magnetic resonance imaging techniques have greatly improved our understanding of lesion activity, the aetiology of MS and the mechanisms underlying lesion formation remain largely unknown. In recent decades, advances in instrumentation and multivariate analysis tools have seen Fourier transform infrared (FTIR) microspectroscopic imaging become a powerful tool for detecting discrete and subtle changes in the macromolecular composition of healthy and diseased tissues. Complementary information about the fundamental structural attributes of the myelin sheath and quantification of the relative amount of myelin within the sample can be determined using small angle X-ray scattering (SAXS). The aim of this thesis was to investigate the biochemical and structural changes underpinning the pathological, developmental and reparative processes in animal models of MS using FTIR microspectroscopic imaging and SAXS. In the first part of this thesis, laboratory based FTIR microspectroscopic imaging, bioinformatics, and synchrotron mapping were used to analyse macromolecular changes in the CNS during the course of experimental autoimmune encephalomyelitis (EAE), an animal model of MS. Using this approach, the distinct and heterogeneous tissue layers of the cerebellum and spinal cord, as well as lesion pathology, could be distinguished from one another. EAE lesions were characterised by low relative lipid concentrations and high relative nucleic acid concentrations and correlated well with regions of demyelination and inflammation identified using conventional histological and immunofluorescence staining techniques. The identification unique infrared 'spectral phenotypes' identified allowed the training of artificial neural networks (ANNs) capable of discriminating EAE pathology from the surrounding healthy tissue, in an unbiased and automated fashion. Moreover, the integration of ANNs with the higher lateral resolution achieved using synchrotron mapping allowed the early detection and definitive identification of microlesions in the CNS of mice, prior to the onset of clinical signs of EAE. Furthermore, the potential of this technique for the evaluation of new therapeutic agents was demonstrated in lesions of animals partially protected against EAE by vaccination with Nogo-A, an inhibitor of neurite outgrowth, where subtle chemical and protein secondary structural changes, not observed by conventional histology, were identified. For the second part of this thesis, FTIR microspectroscopic imaging and SAXS, in conjunction with conventional histological and EM techniques were used to detect and characterise the natural biochemical and ultrastructural changes associated with developmental myelination in the corpus callosum of healthy mice. The onset of myelination was consistently found to occur at postnatal day 14 (P14), while the rate of myelination varied depending on the analytical employed. Myelination reached a maximum rate between P14-P21 and P21-P28 as determined by SAXS and EM, respectively. In contrast, the rate of myelination was found to increase at a constant rate when measured by the relative amount of lipid quantified by FTIR microspectroscopic imaging. In addition to biochemical changes, SAXS analysis revealed that the myelin sheath underwent significant compaction at the extracellular space, which coincided with alterations in protein secondary structure detected in the FTIR spectra. Together, these data suggest that proteins involved in the compaction of the myelin sheath at this site, are responsible for the observed FTIR spectroscopic changes. The identification of significant biochemical changes between the oldest animals (P140) used in this study and the mice agedP98 and younger is of considerable importance, as mice aged between P56-P84are often used in research and thus may still be undergoing significant developmental changes. This is particularly relevant to the CPZ intoxication animal model, which is increasingly being used in MS research to assess demyelinationand remyelination of the corpus callosum of mice aged between P56 and P140. The data obtained here, therefore provide a useful benchmark against which the biochemical and ultrastructural changes occurring in the corpus callosum following CPZ intoxication can be identified and compared. The ability of FTIR microspectroscopic imaging and SAXS to detect and quantify relative biochemical and structural changes during chemically induced demyelination and following subsequent remyelination of the corpus callosum in the CPZ intoxication animal model was examined in the third part of this thesis. Changes in the relative amounts of demyelination and remyelination were easily visualised and quantified in the FTIR spectra using the integrated area of the lipidester carbonyl band as a measure of myelin. Notably, alterations in protein secondary structure were identified following remyelination, suggesting that such differences could be used to identify remyelination in a rapid and automated fashion. Despite these protein conformational changes, the ultrastructure of the myelin sheath, including the widths of the myelin period, lipid bilayers, cytoplasmic space and extracellular space, did not significantly differ during demyelination or remyelination, when compared with the age-matched controls. Interestingly, a discrepancy between the relative amount of myelin measured by SAXS and the average number of myelinated axons within the electron micrographs was found, suggesting that the SAXS technique is only capable of detecting myelin in a highly ordered structure. Thus, the SAXS method applied here could serve as a rapid means for quantifying the relative amount of intact internodal myelin within a sample and could be used to assess the effect of novel therapies on the relative amount of myelin remaining after demyelination or accumulating following remyelination. In summary, the data presented in this thesis illustrates the power of FTIR microspectroscopic imaging and SAXS techniques to detect subtle biochemical and structural changes associated with CNS pathology. These two techniques form a powerful addition to conventional techniques, providing rich biochemical and structural information and a unique opportunity to investigate a range of CNS pathologies within tissues at the molecular level, as well as the potential to evaluate and understand new therapeutic approaches and mechanisms of action
Deamidated wheat protein-carbohydrate Maillard conjugates : effect of size, location and number of carbohydrate conjugated on emulsion steric stabilization at acidic pH and in ionic environment by Benjamin Tziak Ze Wong( )

1 edition published in 2010 in English and held by 1 WorldCat member library worldwide

This study investigated the effects of conjugation of deamidated wheat protein (sIWP - 40,375 Da) with carbohydrate (180 - 41,000 Da) on the protein's solution properties (e.g. pI, aggregation behavior at acidic pH, secondary structure, tryptophan local environment), interfacial properties (e.g. interfacial layer thickness) and the ability to stabilize an oil-in-water (O/W) emulsion at acidic pH and in ionic environments. The protein-carbohydrate conjugates were prepared via the Maillard reaction by dry heating at 60 ðC/75% relative humidity. The carbohydrates used were glucose (180 Da), maltodextrins (900 - 4,300 Da), and dextrans (6,400 - 41,000 Da). Approximately 3 - 4 moles of glucose or low molecular weight (LMW) carbohydrate fraction in the 900 - 4,300 Da maltodextrins (i.e. <425 Da) were conjugated per mole of sIWP, whereas approximately 1.3 mole or 0.5 mole of dextran (6,400 Da or 41,000 Da) was conjugated per mole of sIWP. The zeta-potential and the circular dichroism spectra of the sIWP/protein-conjugates indicate that conjugation did not change the protein's pI (~pH 4) or the protein's secondary structure in solution. sIWP and the protein-conjugates attached with glucose or LMW carbohydrates in the maltodextrins became aggregated in solution when the pH approached the pI. Conversely, protein-conjugates attached with dextrans were aggregated to lower extent in solution than sIWP at its pI due to the conjugated dextran acting as a physical barrier. Dynamic light scattering was used to study the interfacial layer thickness of sIWP/protein-conjugates adsorbed on polystyrene spheres model system. sIWP alone formed a thick protein steric layer of ~18 nm at the interface. Protein-conjugates attached with glucose or LMW carbohydrates in the maltodextrins showed the same layer thickness as sIWP (i.e. ~18 nm), indicating that the attached carbohydrates did not formed an additional carbohydrate steric layer at the interface due to their small sizes. On the other hand, protein-conjugates attached with dextrans (6,400 Da and 41,000 Da) were able to form a thicker interfacial layer by ~3.5 nm and ~5.9 nm respectively than sIWP. Dextranase digestion on the interfacial layer indicates that an additional carbohydrate steric layer was formed by the protein-conjugate attached with 41,000 Da dextran, but was not with 6,400 Da dextran. The observed difference between the attachments of two dextrans with different MW was due to the difference in the sites of conjugation. The smaller 6,400 Da dextran was attached at the C-terminal domain of sIWP, which is the anchoring point to the interface as suggested by the change in the local environment of the tryptophan residues located at the C-terminal domain of sIWP, whereas the larger 41,000 Da dextran was attached at the N-terminal domain of sIWP, as part of the protein diffuse layer. Emulsion stabilized by protein-conjugates attached with LMW carbohydrates in the maltodextrin, or the smaller 6,400 Da dextran became flocculated in acidic pH (pH 4) and in CaCl2 environments (0 - 20 mM), similar to the emulsion stabilized by sIWP. However the emulsion stabilized by the protein-conjugate formed with the larger 41,000 Da dextran was stable in acidic pH (pH 4) and in CaCl2 environments (0 - 20 mM). This is due to the additional carbohydrate steric layer formed by the attached dextran which provided adequate steric stability against emulsion droplets flocculation. This study showed that Maillard conjugation can be used to improve the ability of protein (e.g. sIWP) in stabilizing emulsions at acidic pH and in ionic environments by forming an additional carbohydrate steric layer at the interface. However the formation of an effective carbohydrate steric layer is dependent on the size of the carbohydrate, the location of conjugation and the number of carbohydrate conjugated.[Appendices not included]
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1 edition published in 2018 in English and held by 1 WorldCat member library worldwide

 
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Languages
English (11)