WorldCat Identities

Lesieur, Sylviane

Overview
Works: 26 works in 31 publications in 2 languages and 45 library holdings
Roles: Thesis advisor, Opponent, Contributor, Author, Other
Classifications: QH345, 547.7
Publication Timeline
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Most widely held works by Sylviane Lesieur
ETUDE DE LA SOLUBILISATION ET DE LA FORMATION DE VESICULES NON-IONIQUES EN PRESENCE D'OCTYL B-D-GLUCOPYRANOSIDE by M Seras( Book )

2 editions published in 1992 in French and held by 5 WorldCat member libraries worldwide

LA COMPREHENSION DES MECANISMES DE SOLUBILISATION DES MEMBRANES BIOLOGIQUES OU LA MAITRISE DES PROCESSUS DE LEUR RECONSTITUTION PASSENT PREALABLEMENT PAR LA CONNAISSANCE DE SYSTEMES MODELES LIPIDE(S)-DETERGENT(S). DANS CE CADRE, LES DETERGENTS NON IONIQUES ONT ETE LARGEMENT UTILISES ET, PLUS PARTICULIEREMENT, L'OCTYL B-D-GLUCOPYRANOSIDE (OG) POUR SES PROPRIETES NON DENATURANTES VIS-A-VIS DES PROTEINES ET LA VALEUR RELATIVEMENT ELEVEE DE SA CONCENTRATION MICELLAIRE CRITIQUE (CMC). LA SOLUBILISATION ET LA FORMATION, EN PRESENCE D'OG, DE VESICULES NON IONIQUES COMPOSEES D'UN MELANGE EQUIMOLAIRE DE CHOLESTEROL ET D'UN LIPIDE MONOCATENAIRE NON IONIQUE, L'HEXADECYLETHER DE DIGLYCEROL, AVEC UNE FAIBLE PROPORTION D'UNE MOLECULE CHARGEE, LE DICETYLPHOSPHATE, A ETE ABORDEE A DEUX NIVEAUX: I) AU NIVEAU SUPRAMOLECULAIRE EN UTILISANT LES TECHNIQUES DE DIFFUSION DE LA LUMIERE ET DE MICROSCOPIE ELECTRONIQUE PAR CRYO-TRANSMISSION; II) AU NIVEAU MOLECULAIRE EN SUIVANT L'EMISSION DE FLUORESCENCE STATIQUE DE DEUX SONDES MEMBRANAIRES ET RESOLUE DANS LE TEMPS D'UN ANALOGUE DU CHOLESTEROL INCORPORE DANS LES MEMBRANES. PARALLELEMENT, L'INFLUENCE DE LA CINETIQUE D'ADDITION OU D'ELIMINATION DU DETERGENT A ETE ETUDIEE. IL RESSORT DE CETTE ETUDE QUE LA TRANSFORMATION DES VESICULES NON IONIQUES EN MICELLES EST PRINCIPALEMENT REGIE PAR DES PROCESSUS MOLECULAIRES CINETIQUEMENT LIMITES PAR L'INSERTION ET LA DIFFUSION DU DETERGENT DANS LES MEMBRANES. AU CONTRAIRE LA FORMATION DES VESICULES EST GOUVERNEE PAR LES REARRANGEMENTS SUPRAMOLECULAIRES DES AGREGATS, L'EQUILIBRE MOLECULAIRE ETANT ATTEINT INSTANTANEMENT
Liposomes superparamagnétiques stabilisés stériquement pour l'imagerie IRM et la thérapie des cancers à tumeurs solides : étude in vitro et in vivo de la vectorisation magnétique d'un anti-ostrogène sur un modèle tumoral de cancer du sein by Vincent Plassat( Book )

2 editions published in 2010 in French and held by 3 WorldCat member libraries worldwide

Ce travail porte sur l'utilisation de liposomes superparamagnétiques PEG-ylés pour la vectorisation par voie systémique d'agents anticancéreux dans des tumeurs solides sous gradient de champ magnétique. La première partie établit les paramètres pharmacocinétiques et la biodistribution du vecteur chez la souris après administration intraveineuse. Les liposomes chargés de fluide magnétique ou MFLs présentent en particulier un temps de circulation prolongé avec une demi-vie de 12,5h. La deuxième partie concerne le traitement de tumeurs du cancer du sein issues de la lignée humaine MCF-7 à l'aide de l'anti -œstrogène RU 58668 préalablement incorporé dans la bicouche lipidique des MFLs. La structure mixte RU 58668/MFL ou RU-MFLs est parfaitement stable comme démontré par calorimétrie et diffraction des rayons X. L'étude in vitro reposant sur différentes techniques, notamment microscopies confocale de fluorescence et électronique ou magnétophorèse, révèle que le processus d'internalisation des RU-MFLs au sein de cellules MCF-7 obéit à un mécanisme d'endocytose préservant la structure vésiculaire des liposomes. Le rendement d'association est significativement amélioré en présence d'un gradient de champ magnétique produit par un aimant placé au voisinage des cellules. L'étude in vivo sur des souris porteuses de tumeurs xenogreffées valide définitivement l'efficacité de la vectorisation magnétique via le compartiment vasculaire à l'appui d'une caractérisation poussée associant imagerie par résonance magnétique (IRM), fibroscopie de fluorescence, biodistribution, analyse histologique et immunohistochimie. L'ensemble de ce travail montre que la vectorisation magnétique d'un anti-œstrogène par les MFLs apporte potentiellement un réel bénéfice pour traiter les tumeurs de cancers du sein hormono-dépendants. De façon plus générale, les MFLs apparaissent comme un outil très prometteur pour le traitement par voie systémique des cancers à tumeurs localisées en ayant l'avantage de pouvoir coupler thérapie et diagnostic puisque le matériel magnétique encapsulé assure non seulement le guidage des liposomes mais représente un agent de contraste intrinsèque pour l'IRM
PROPRIETES STRUCTURALES DE SYSTEMES MIXTES NON IONIQUES LIPIDE-TENSIOACTIF : - ELABORATION DE VESICULES STABILISEES STERIQUEMENT - CONTRIBUTION A L'ETUDE DE LA TRANSITION MICELLES VESICULES by Sophie Beugin Deroo( Book )

2 editions published in 1997 in French and held by 3 WorldCat member libraries worldwide

LA PREMIERE PARTIE DE CE TRAVAIL PORTE SUR LA CARACTERISATION PHYSICO-CHIMIQUE D'UNE NOUVELLE CLASSE DE VESICULES NON-IONIQUES STABILISEES STERIQUEMENT, A BASE D'HEXADECYLETHER DE DIGLYCEROL (C#1#6G#2), DE CHOLESTEROL ET DE CARBONATE DE MONOMETHOXYPOLY(ETHYLENE GLYCOL) (M-PEG-CHOL). LA SYNTHESE DU POLYMERE A ETE MISE AU POINT. LES COMPOSITIONS LIPIDIQUES ONT ETE CHOISIES A L'AIDE D'ETUDES STRUCTURALES REALISEES PAR ANALYSE THERMIQUE DIFFERENTIELLE ET DIFFRACTION DES RAYONS X. LES VESICULES ONT ETE ANALYSEES PAR MICROSCOPIE ELECTRONIQUE PAR CRYO-TRANSMISSION, DIFFUSION QUASI-ELASTIQUE DE LA LUMIERE, CHROMATOGRAPHIE D'EXCLUSION SUR GEL, TURBIDIMETRIE ET SPECTROPHOTOMETRIE DE FLUORESCENCE. LE DIAGRAMME C#1#6G#2/CHOLESTEROL EN EXCES D'EAU REVELE NOTAMMENT AUX COMPOSITIONS INTERMEDIAIRES UNE PHASE MIXTE UNIQUE ORGANISEE EN BICOUCHES. LES STRUCTURES C#1#6G#2/CHOLESTEROL/M-PEG-CHOL ONT ETE ETUDIEES EN FONCTION DE L'HYDRATATION, DE LA TENEUR EN POLYMERE ET DE LA LONGUEUR DE LA CHAINE PEG. LE POLYMERE, ANCRE DANS LA BICOUCHE, INDUIT UN GONFLEMENT LAMELLAIRE JUSQU'A UN SEUIL DE SATURATION, AU-DELA DUQUEL LES STRUCTURES LAMELLAIRES DISPARAISSENT AU PROFIT D'OBJETS DISCOIDAUX. DES COMPOSITIONS EN POLYMERE INFERIEURES A CE SEUIL PERMETTENT DE PREPARER DES VESICULES UNILAMELLAIRES, IMPERMEABLES, STABLES ET RESISTANTES AUX AGENTS SOLUBILISANTS. LEUR MORPHOLOGIE EST LIEE A LA CONFORMATION DU PEG A LEUR SURFACE. LA SECONDE PARTIE DE LA THESE CONCERNE L'ANALYSE DES PHASES DONT SONT ISSUES LES STRUCTURES IMPLIQUEES DANS LA SOLUBILISATION DE VESICULES DE PHOSPHATIDYLCHOLINE PAR UN TENSIOACTIF GLUCOSIDIQUE, L'OCTYL GLUCOSIDE OU L'HECAMEG. POUR DES TENEURS CROISSANTES EN TENSIOACTIF, NOUS AVONS SUCCESSIVEMENT OBSERVE DES STRUCTURES LAMELLAIRES HETEROGENES PUIS HOMOGENES, UN DOMAINE TRIPHASIQUE (LAMELLAIRE, MICELLAIRE, EAU), UNE PHASE DE MICELLES VERMICULAIRES EXCLUANT DE L'EAU PUIS UN DOMAINE PUREMENT MICELLAIRE
Direct synthesis of novel amphiphilic cyclodextrin by Delphine Gallois-Montbrun( )

1 edition published in 2007 in English and held by 2 WorldCat member libraries worldwide

Designing nanoparticles for delivery of neurotrophic proteins by Angelina Angelova( )

1 edition published in 2013 in English and held by 2 WorldCat member libraries worldwide

Liposome retention in size exclusion chromatography by Tristan Ruysschaert( )

1 edition published in 2005 in English and held by 2 WorldCat member libraries worldwide

Conception de magnétoliposomes furtifs pour le diagnostic et la thérapie anti-cancéreuse by Marie Sophie Martina( Book )

2 editions published in 2006 in French and held by 2 WorldCat member libraries worldwide

Un nouveau système hybride résultant de l'encapsulation de nanoparticules d'oxyde de fer superparamagnétiques dans des vésicules phospholipidiques stabilisées stériquement par des chaînes de poly(éthylène glycol) (PEG) a été mis au point et caractérisé. Ces magnétoliposomes, stables et biocompatibles sont d'excellents agents de contraste pour l'imagerie par résonance magnétique (IRM) qui, par application d'un gradient de champ magnétique externe, peuvent être guidés in vivo dans le tissu tumoral ou un hémisphère cérébral. In vitro, la présence de chaînes de PEG réduit fortement l'association magnétoliposome/macrophages (lignée J774) sans pour autant modifier la cinétique d'endocytose des magnétoliposomes une fois ceux-ci liés à la surface des cellules. Dans le cas de cellules d'adénocarcinome prostatique humain, il a été montré que l'internalisation cellulaire des magnétoliposomes pouvait être nettement favorisée en présence d'un gradient de champ magnétique
Thermotropic phase behavior of in vivo extracted human stratum corneum lipids by Frédéric Bonté( )

1 edition published in 1997 in English and held by 2 WorldCat member libraries worldwide

Rapid cooling of lipid in a prilling tower Theoretical considerations and consequences on the structure of the microspheres by Perrine Pivette( )

1 edition published in 2009 in English and held by 2 WorldCat member libraries worldwide

Water behaviour in processed cheese spreads DSC and ESEM study by Hela Gliguem( )

1 edition published in 2009 in English and held by 2 WorldCat member libraries worldwide

Rapid mixing stopped-flow small-angle X-ray scattering study of lipoplex formation at beamline ID02ESRF by Borislav Angelov( )

1 edition published in 2015 in English and held by 2 WorldCat member libraries worldwide

<> by Valérie Bernat( Book )

1 edition published in 2008 in French and held by 2 WorldCat member libraries worldwide

Preface by Sylviane Lesieur( )

1 edition published in 2009 in English and held by 2 WorldCat member libraries worldwide

Elaboration et caractérisation d'édifices supramoléculaires mixtes cyclodextrine/lipide : cristallogénèse et analyse structurale de complexes d'inclusion, nano-assemblages de dérivés covalents by Delphine Gallois-Montbrun( Book )

2 editions published in 2009 in French and held by 2 WorldCat member libraries worldwide

This thesis work treats with the preparation of cyclodextrin and lipid based self assembled nanomaterials and its characterisation from the micro level down to the atomic level. Two different strategies have been developed for the sake of nanomaterial preparation. The first one consisted of the preparation of non covalent inclusion complexes where alpha-cyclodextrins were host molecules for single chained lipids. Four different columnar structures have been identified for the latter complexes using powder X-ray diffraction techniques. It was found that these nanomaterial structures are influenced by the i) lipid hydrocarbon chain length ii) the nature of the polar head group and iii) crystallisation conditions such as temperature of complexation reaction or crystallization kinetics. It was also shown that the compactness of the molecular structure is also a function of the hydration state of the complex (amount of bound water molecules in the structure). The characterisation of single crystals of some of those inclusion compounds, using both neutron and X-ray diffraction, permitted the determination of the molecular structure and hence the description of hydrogen bounds in the structure. The second strategy employed the synthesis of covalently bound fatty acids onto the primary face of bêta-cyclodextrin. In this fashion, statistically substituted amphiphiles have been obtained. Their capacities to self assemble in the aqueous phase for the formation of nanoparticles has been determined after their composition and degree of purity had been established. Finally the formulation condition permitting stable aqueous dispersions have been developed
Stealth nanoparticles for preclinical X-rays imaging and multimodal X-rays/MRI (magnetic resonance imaging) imaging by Justine Wallyn( )

1 edition published in 2017 in English and held by 1 WorldCat member library worldwide

Biomedical imaging is nowadays an essential tool to establish a diagnosis by means of observation of tissues and biological fluids. Combination of imaging instrument with contrast enhancers is a key to obtain clear delineation of a desired tissue by accumulation of a contrast agent into this specific target. The two main imagers are the X-ray scanner and the magnetic resonance imaging (MRI).These imagers are frequently used in conjuncture. Typically, small hydrosoluble iodinated molecules are used as contrasting material for radiography whereas MRI involves magnetic materials like iron oxide nanoparticles. In this work, we proposed two novel contrast agents, the first one was aiming to form an alternative to iodinated contrast agents suffering from fast excretion and causing renal toxicity whereas the second one was aiming at providing bimodal contrasting ability to facilitate access to bimodal imaging procedure in clinics. In the first case, iodinated polymeric nanoparticles, serving for preclinical X-ray imaging were formulated by nanoprecipitation technique. Parameters of formulation were elucidated to provide nanoparticles with size distribution suitable for in vivo administration and high iodine content for contrast enhancement. In vivo study revealed the efficacy of our nanoparticles to clearly visualize liver and spleen and limiting current issues associated with marketed radiopaque contrast agents. The second work achieved was aiming at formulating bimodal lipids-based nanocarriers capable of yielding contrast enhancement for X-ray imaging and MRI by combining iodinated oil and iron oxide nanoparticles within a nano-emulsion core. This would provide bimodal nanoparticulate platform to carry out fast and efficient dual modal imaging procedures. In this context we succeeded to generate efficient dual modal contrast agent yielding clear visualization of liver and kidney by MRI and liver and spleen by X-ray imaging. Pharmacokinetic profile was so determined thanks to bimodal imaging. Using MRI allowed to show that kidneys eliminated a fraction of the dose whereas X-ray imaging confirmed that both tissues, liver and spleen, were passively targeted. These two studies proposed solutions limiting current issues of radiopaque contrast agents and novel formulations to facilitate bimodal imaging for soft tissues imaging
A scalable process to produce lipid-based compartmented Janus nanoparticles with pharmaceutically approved excipients1( )

1 edition published in 2018 in English and held by 1 WorldCat member library worldwide

Abstract : In the field of nanotechnologies, theranostic approaches and fixed-dose combination products require the development of innovative carriers able to co-encapsulate several entities of interest. Abstract : In the field of nanotechnologies, theranostic approaches and fixed-dose combination products require the development of innovative carriers able to co-encapsulate several entities of interest. This communication describes the preparation and characterization of lipid-based Janus compartmented nanoparticles. They were successfully prepared using a scalable process with pharmaceutically approved excipients. The analysis of the microscopic structure and supramolecular organization demonstrated the formation of two physico-chemically different compartments enabling the co-administration at once of both liposoluble and hydrosoluble active pharmaceutical ingredients
Technologie de prilling : des principes fondamentaux du procédé au développement de microsphères lipidiques by Floriane Séquier( )

1 edition published in 2013 in French and held by 1 WorldCat member library worldwide

This work was conducted within the framework of an industrial collaboration and focused on the prilling technology and the development of lipid microparticles by this original technique.The first part of this work was to select lipid excipients and to study their behavior in the two main steps of the process: the droplet's formation by vibrating nozzle and the droplet's crystallization by quenching in the prilling tower. These studies highlight the critical key parameters involves in the success of the process and therefore the quality of the final product: viscosity and thermal and structural properties of lipids. These parameters should be therefore systematically studied before considering their application to technology prilling.The second part of this work concerns the formulation of three active molecules by prilling: Ketoprofen, Dronedarone and Fexofenadine. These three examples were used to illustrate different ways of incorporating the active ingredient in the process and their associated problems: the melting, the solubilisation and the suspension of active molecules in melted lipids
Reconstitution of non-ionic monoalkyl amphiphile-cholesterol vesicles by dilution of lipids-octylglucoside mixed micelles by M Seras( Book )

1 edition published in 1993 in English and held by 1 WorldCat member library worldwide

Nanoformulations pour la protection de flavonoïdes instables : exemple de la quercétine by Tri Truong Công( )

1 edition published in 2012 in French and held by 1 WorldCat member library worldwide

Cette thèse porte sur la mise au point de formulations de nanoparticules lipidiques à base de polyoxylglycérides afin d'assurer la protection de principes actifs instables chimiquement et physiquement, la quercétine (un flavonoïde antioxydant fragile) dans le cas présent. Différents systèmes dispersés ont été préparés par homogénéisation haute pression à chaud avec une taille des particules blanches entre 100 - 200 nm. Ces nanodispersions sont très stables sur plusieurs années à température ambiante. L'encapsulation de la quercétine, dans les nanoparticules lipidiques multicompartimentées et la préparation de nanocristaux ont permis d'augmenter fortement sa teneur dans la dispersion et d'améliorer effectivement sa stabilité physico-chimique
Évaluation et optimisation du potentiel thérapeutique d'un dendrimère anti-inflammatoire par sa formulation dans des vésicules catanioniques pour le traitement topique du psoriasis by Ranime Jebbawi( )

1 edition published in 2021 in French and held by 1 WorldCat member library worldwide

Psoriasis is a chronic inflammatory skin disease. It is characterized by an abnormal proliferation of keratinocytes, dilation of dermal blood vessels and an infiltration of inflammatory cells into the skin. Although effective, the side effects of anti-psoriatic treatments limit their long-term use. A Poly(PhosphorHydrazone) dendrimer capped with AzaBisPhosphonate groups, called ABP, has previously been shown to have anti-inflammatory properties both in vitro and in animal models of chronic inflammatory diseases. The aim of our study is to assess and optimize the therapeutic potential of ABP dendrimer as a new approach for psoriasis therapy. We have shown that ABP dendrimer applied topically in physiological buffer, without specific formulation, significantly reduced the inflammation induced by imiquimod in mice. However, the therapeutic efficacy of the ABP dendrimer for the topical treatment of psoriasis should be improved. The barrier properties of the stratum corneum and physicochemical properties of the ABP dendrimer both contribute to limit its skin penetration and therefore its therapeutic efficacy by topical route. In order to maximize its therapeutic effects, we have formulated the dendrimer in an innovative delivery system, sugar- derived tricatenar catanionic vesicles. These catanionic vesicles, developed in our team, are formed from a bilayer of two opposite charges surfactants. In addition to their biocompatibility, these catanionic vesicles have previously demonstrated their ability to promote skin penetration of active ingredients when their bilayer is in fluid state. After optimization of dendrimer formulation in catanionic vesicles, we obtained stable vesicles capable of encapsulating approximately 80% of the dendrimer. We have also shown that the insertion of the dendrimer into the vesicular bilayer makes it fluid at skin temperature. Our studies ex vivo have successfully shown that the formulation of dendrimer in catanionic vesicles allows deep skin penetration of the dendrimer into the viable epidermis and dermis. Finally, we have demonstrated that TriCat / ABP-A formulation improves the efficacy of dendrimer in murine model of psoriasis induced by imiquimod. Altogether, our results highlight the potential of this innovative formulation to deliver dendrimer to the deeper skin layers for the treatment of psoriasis
 
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Languages
French (14)

English (11)