WorldCat Identities

Sanchez, Wilfried

Overview
Works: 24 works in 27 publications in 2 languages and 41 library holdings
Roles: Contributor, Opponent, Other, Author, Thesis advisor
Publication Timeline
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Most widely held works by Wilfried Sanchez
Biomarkers as tools for monitoring within the Water Framework Directive context: concept, opinions and advancement of expertise by Thomas Milinkovitch( )

1 edition published in 2019 in English and held by 2 WorldCat member libraries worldwide

Approche multi-biomarqueurs chez l'épinoche à trois épines (Gasterosteus aculeatus L.) : un modèle pour la surveillance des écosystèmes aquatiques continentaux by Wilfried Sanchez( Book )

2 editions published in 2007 in French and held by 2 WorldCat member libraries worldwide

The aim of this work was to characterise the usefullness of three-spined stickleback (Gasterosteus aculeatus L.) as a sentinel fish species to assess aquatic environment contamination using a set of biomarkers. For this purpose, assays for biomarkers of xenobiotic metabolisation, oxidative stress, neurotoxicity and estrogenic or androgenic endocrine disruption have been developed and validated. Our laboratory and field results show that investigated biomarkers are sensitive and inducible parameters. To run the full potential of biomarkers, basal values mesured in reference site are established and their seasonal or gender variability is characterised. Field application provides information on early effects of contamination. Moreover, it provides complementary informations compared to chemical analysis and populational investigations and allows sampling site discrimination. At least, the development of a biomarker-based index allows the synthesis of collected data and offers a perspective for using biomarkers in aquatic ecosystem management
Recycling, reuse, and circular economy: a challenge for ecotoxicological research by Vera I Slaveykova( )

1 edition published in 2019 in English and held by 2 WorldCat member libraries worldwide

A potential biomarker of androgen exposure in European bullhead (Cottus sp.) kidney by Mélanie Villeret( )

1 edition published in 2012 in English and held by 2 WorldCat member libraries worldwide

Acclimation capacity of the three-spined stickleback (Gasterosteus aculeatus, L.) to a sudden biological stress following a polymetallic exposure by Antoine Le Guernic( )

1 edition published in 2016 in English and held by 2 WorldCat member libraries worldwide

The European technical report on aquatic effect-based monitoring tools under the water framework directive by Ann-Sofie Wernersson( )

1 edition published in 2015 in English and held by 2 WorldCat member libraries worldwide

In situ experiments to assess effects of constraints linked to caging on ecotoxicity biomarkers of the three-spined stickleback (Gasterosteus aculeatus L.) by Antoine Le Guernic( )

1 edition published in 2015 in English and held by 2 WorldCat member libraries worldwide

Former uranium mine-induced effects in caged roach: a multiparametric approach for the evaluation of in situ metal toxicity by Béatrice Gagnaire( )

1 edition published in 2014 in English and held by 2 WorldCat member libraries worldwide

Ecotoxicology and toxicology: problems and decisions by Muriel Bost( )

1 edition published in 2018 in English and held by 2 WorldCat member libraries worldwide

Développement d'un biomarqueur de qualité spermatique chez deux espèces de crevettes Palaemonidae : état des lieux le long du continuum estuaire / littoral de la Seine by Alexandre Erraud( )

2 editions published in 2018 in French and held by 2 WorldCat member libraries worldwide

The environment shapes the reproduction's strategy and performance of a population, influencing its fitness and a fortiori its survival. Therefore, biomarkers that alter reproductive functions represent a great interest in ecotoxicology. The reduction of male fertility in wildlife can represent a threat to the population's survival. Moreover, few methodologies are available for species that are relevant to address this issue on environmental biomonitoring programs, especially for crustaceans, despite their representativeness in the wildlife and their essential functions within ecosystems. The present work aimed to propose methodologies based on the measurement of functionality and integrity spermatic biomarkers on Palaemonidae shrimps. We studied two species, an estuarine, Palaemon longirostris, and a coastal species, Palaemon serratus, due to their complementarity on the continuum estuary-littoral ecosystem. However, crustaceans' sperm has many structural and functional characteristics, the number of transposable markers is limited. Thereafter, the research effort was refocused on the measurement of the DNA integrity and this method was evaluated for its adequacy for a biomonitoring study. We optimized and validate the Comet assay for the Palaemonidae species, and the dynamic of the biological response in terms of appearances, persistence, and possible effects on fitness was evaluated under controlled conditions in the laboratory. Furthermore, ex-vivo and in-vivo exposures were conducted using genotoxic models with different modes of action. On the one hand, results attested to the sensibility, the reproducibility and the integrating character of the response, on the other hand, no correlation between damaged sperm DNA and an alteration of the pre-hatch stage of development was established. In parallel, an in-situ approach was conducted to characterize the baseline level of the measured response, taking into consideration the specific constraints of each species' habitat. We were able to define a common baseline level and a threshold value for both species, highlighting the potential of the tool for inter-species transferability. This method was tested with native populations from the estuary and from the Seine Bay in 2015 and 2016. And, the results proved to be consistent with the contamination pressure and the hydro-sedimentary dynamics of the Seine Bay. Ultimately, the DNA integrity of sperm in Palaemonidae seems to be functional for in-situ deployments as a biomarker of exposure to genotoxic stress. Nevertheless, future studies should be conducted (1) to better discern the implications of this spermatic damage on the recruitment of new cohorts and, (2) to test the transferability of the methodology to other shrimp species and on a wider geographical scale
In situ effects of metal contamination from former uranium mining sites on the health of the three-spined stickleback (Gasterosteus aculeatus, L.) by Antoine Le Guernic( )

1 edition published in 2016 in English and held by 2 WorldCat member libraries worldwide

Juvenile roach (Rutilus rutilus) increase their anaerobic metabolism in response to copper exposure in laboratory conditions by Virginie Maes( )

1 edition published in 2016 in English and held by 2 WorldCat member libraries worldwide

Depleted Uranium Disturbs Immune Parameters in Zebrafish, Danio rerio: An Ex Vivo/In Vivo Experiment by Béatrice Gagnaire( )

1 edition published in 2014 in English and held by 2 WorldCat member libraries worldwide

Health and environmental risks associated with emerging pollutants and novel green processes by Wilfried Sanchez( )

1 edition published in 2018 in English and held by 2 WorldCat member libraries worldwide

Un biomarqueur de génotoxicité chez l'épinoche (Gasterosteus aculeatus) : application au biomonitoring et étude de sa valeur prédictive en écotoxicologie by Raphaël Santos( )

2 editions published in 2013 in French and held by 2 WorldCat member libraries worldwide

A rule context in the field of environmental impact assessment of anthropogenic chemicals and specifically ecotoxicological risk to the aquatic environment, requires strengthening the assessment tools and refine knowledge of their predictive value. As such, the study of biomarkers of genotoxicity should be choose, given the central role of DNA in living organisms function and trans-generational effects could lead to a genetic load affecting populations exposed to a contaminated environment . In this context, and after defining the problem at the end of a literature review, this thesis has developed a biomarker of genotoxicity in the stickleback (Gasterosteus aculeatus), model species in ecotoxicology, using the measurement primary DNA damage by the comet assay under alkaline conditions. It aimed to: i) provide additional information in a battery of biomarkers used in biomonitoring in this species, 2) provide some understanding of the predictive value of a genotoxic effect on the germinal tissue of aquatic organisms exposed to chemical stress. On the first point, the results underline the importance of this biomarker of genotoxicity developed on blood tissue in the laboratory, its high sensitivity leading to a significant discriminating power under multiparameter approaches taken in a second time on the ground. Regarding the study of the ecotoxicological significance of this biomarker of genotoxicity, the aim was to explore the relationship between DNA integrity in germ cells of the fish and the quality of the offspring. The results of this work show an original way the majority of the paternal genome contribution to the transmission of a deleterious genetic information that result in abnormal survival and development measured on the offspring. They allow you to have a predictive element relevant evaluation of the effect of genotoxic potential contaminants in aquatic ecosystems, in a context of growing interest in understanding the decline of fish populations identified universally
Le chabot comme espèce modèle pour évaluer les effets des perturbateurs endocriniens by Mélanie Villeret( )

1 edition published in 2012 in French and held by 1 WorldCat member library worldwide

L'objectif de ce travail était d'évaluer le potentiel du chabot (Cottus sp.) comme espèce modèle pour l'étude des effets des perturbateurs endocriniens (PE) in situ. Pour y parvenir, ce travail a débuté par l'étude de la reproduction des populations sauvages de chabots. En effet, le chabot présentant un mode de reproduction complexe, cette étape nous a semblé être un pré-requis indispensable avant d'envisager l'utilisation de cette espèce en tant qu'indicateur de la contamination par des perturbateurs endocriniens. Cette première étape a permis de confirmer l'existence de deux stratégies reproductives chez les chabots (annuelle ou saisonnière) dans notre zone d'étude inhérente au programme INTERREG et a mis en évidence les facteurs environnementaux qui en sont responsables. Par ailleurs, cette étude a permis la confirmation d'une hypertrophie dans le rein des chabots mâles en période de reproduction et la mise au point d'une méthode histologique permettant de la quantifier : la mesure de l'épaisseur de l'épithélium rénal (KEH). Ce travail a ensuite consisté à caractériser les niveaux de réponses mesurés chez le chabot en conditions contrôlées et en contexte terrain. Les études de laboratoire ont permis de statuer sur l'inductibilité ainsi que la sensibilité et la spécificité de l'hypertrophie rénale à l'aide de substances de références androgéniques ou non androgéniques. Les investigations in situ ont servi à évaluer le pouvoir discriminant de cet indicateur en contexte de pollution environnementale mais également de statuer sur le potentiel du chabot comme espèce modèle pour évaluer les effets des PE à l'aide d'autres marqueurs reflétant la perturbation endocrinnienne. Les résultats ont mis en évidence que l'utilisation de la méthode KEH chez le chabot comme un indicateur d'exposition aux androgènes n'était pas pertinente aussi bien au laboratoire que sur le terrain. En effet, il est apparu être faiblement inductible et moins sensible que ceux déjà disponible chez d'autres espèces. De plus, les profils de réponses observés sur le terrain n'ont pas permis une discrimination des sites de références et des sites contaminés tout comme les réponses des autres marqueurs utilisés. En résumé ce travail démontre le faible potentiel du chabot pour renseigner sur la perturbation endocrinnienne. Les efforts doivent néanmoins se poursuivre pour identifier et caractériser les protéines inductibles par les androgènes responsables des modifications histologiques afin de développer et valider une méthode de dosage quantitative spécifique et sensible utilisable comme biomarqueur d'androgénicité qui permettrait d'envisager l'utilisation du chabot comme espèce sentinelle utilisable pour la surveillance des milieux aquatiques
The effects of environmental stressors on coastal fish : in situ and experimental approach by Isil Filipuci( )

1 edition published in 2011 in English and held by 1 WorldCat member library worldwide

Les estuaires et les zones côtières constituent des territoires à fort enjeux stratégiques économiquement et pour l'environnement. Ils assurent de nombreuses fonctions biologiques et écologiques dont celle de nourricerie et de frayère pour les poissons. Ces écosystèmes sont pourtant soumis à de multiples facteurs de stress, à la fois naturels et anthropogéniques, qui peuvent représenter une menace potentielle envers les organismes aquatiques, en particulier pour les espèces commerciales de poissons. Dans ce contexte, les effets de facteurs de stress environnementaux tels que la contamination chimique et les efflorescences algales nuisibles (HABs) ont été étudiés par des approches in situ et expérimentale (microcosme et mésocosme) sur deux espèces de poissons : le flet (Platichthys flesus) et le bar (Dicentrarchus labrax). Durant cette thèse, nous avons utilisé différents indicateurs pour déterminer les réponses des poissons aux stress environnementaux. Parmi ces outils, nous avons utilisés des indices de croissance et condition, des biomarqueurs moléculaires et des paramètres immunologiques
An integrative approach to assess ecological risks of surface water contamination for fish populations( )

1 edition published in 2017 in English and held by 1 WorldCat member library worldwide

Abstract: Contamination of aquatic ecosystems is considered as one of the main threats to global freshwater biodiversity. Within the European Water Framework Directive (EU-WFD) a particular attention is dedicated to assess ecological risks of surface water contamination and mitigation of chemical pressures on aquatic ecosystems. In this work, we evaluated ecological risks of surface water contamination for fish populations in four EU-WFD rivers through an integrative approach investigating three Lines of Evidence (chemical contamination, biomarker responses as early warning signals of contamination impacting individuals and ecological analyses as an indicator of fish community disturbances). This work illustrates through 4 case studies the complementary role of biomarkers, chemical and ecological analyses which, used in combination, provide fundamental information to understand impacts of chemical pressures that can affect fish population dynamics. We discuss the limitations of this approach and future improvements needed within the EU-WFD to assess ecological risk of river contamination for fish populations. Graphical abstract: Highlights: Integrated approach improve ERA implemented within the EU-WFD. Chemistry, biomarkers and ecological analyses are complementary LOE. Biomarkers point out biological disturbances before fish community are disturbed. Chemical, Biomarker and Ecological indexes improve decision making by managers
 
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English (16)

French (7)