WorldCat Identities

Gagnaire, Béatrice

Overview
Works: 17 works in 18 publications in 2 languages and 27 library holdings
Roles: Author, Contributor, Opponent, Other, Thesis advisor
Publication Timeline
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Most widely held works by Béatrice Gagnaire
Effects of in vivo exposure to tritium: a multi-biomarker approach using the fathead minnow, Pimephales promelas by Béatrice Gagnaire( )

1 edition published in 2018 in English and held by 2 WorldCat member libraries worldwide

Former uranium mine-induced effects in caged roach: a multiparametric approach for the evaluation of in situ metal toxicity by Béatrice Gagnaire( )

1 edition published in 2014 in English and held by 2 WorldCat member libraries worldwide

Depleted Uranium Disturbs Immune Parameters in Zebrafish, Danio rerio: An Ex Vivo/In Vivo Experiment by Béatrice Gagnaire( )

1 edition published in 2014 in English and held by 2 WorldCat member libraries worldwide

In situ experiments to assess effects of constraints linked to caging on ecotoxicity biomarkers of the three-spined stickleback (Gasterosteus aculeatus L.) by Antoine Le Guernic( )

1 edition published in 2015 in English and held by 2 WorldCat member libraries worldwide

Étude des effets des polluants sur les paramètres hémocytaires de l'huître creuse Crassostrea gigas : interactions entre environnement, mécanismes de défense et maladies infectieuses by Béatrice Gagnaire( Book )

2 editions published in 2005 in French and held by 2 WorldCat member libraries worldwide

L'ostréiculture est principalement réalisée dans les estuaires, zones soumises à de nombreuses pollutions liées aux activités anthropiques. Parmi ces animaux, l'huître creuse, Crassostrea gigas, représente un modèle d'intérêt car elle est sédentaire et filtre de grandes quantités d'eau pour se nourrir. Parmi toutes les fonctions physiologiques pouvant être perturbées par les polluants, les mécanismes de défense sont peu étudiés chez les bivalves. Des animaux présentant des activités de défense altérées peuvent se montrer plus sensibles aux maladies infectieuses. Dans ce contexte, les effets de polluants sur les activités hémocytaires et la sensibilité aux maladies ont été testés chez C. gigas. Après mise au point de protocoles permettant de suivre des paramètres hémocytaires en cytométrie de flux, des expériences de contamination ont été conduites in vitro et in vivo. Les expériences in vitro ont démontré les effets de plusieurs polluants sur les paramètres hémocytaires. La contamination in vivo avec un mélange de pesticides a induit une diminution de l'activité de phagocytose et une sous-expression de gènes immunitaires mesurée par PCR en temps réel. Dans d'autres expériences impliquant la même contamination suivie d'une injection de bactéries, la mortalité était plus élevée et plusieurs gènes immunitaires étaient sur-exprimés chez les animaux contaminés infectés en comparaison aux animaux non contaminés infectés. Les expériences in situ ont permis de définir le statut de défense de différentes populations génétiques. Cette étude est la première à explorer une relation entre modulation des paramètres hémocytaires et dérèglement de la réponse aux bactéries chez C. gigas
In situ effects of metal contamination from former uranium mining sites on the health of the three-spined stickleback (Gasterosteus aculeatus, L.) by Antoine Le Guernic( )

1 edition published in 2016 in English and held by 2 WorldCat member libraries worldwide

Effects of Depleted Uranium on Oxidative Stress, Detoxification, and Defence Parameters of Zebrafish Danio rerio by Beatrice Gagnaire( )

1 edition published in 2012 in English and held by 2 WorldCat member libraries worldwide

Isolation and primary culture of gill and digestive gland cells from the common mussel Mytilus edulis by Jérôme Faucet( )

1 edition published in 2004 in English and held by 2 WorldCat member libraries worldwide

Acclimation capacity of the three-spined stickleback (Gasterosteus aculeatus, L.) to a sudden biological stress following a polymetallic exposure by Antoine Le Guernic( )

1 edition published in 2016 in English and held by 2 WorldCat member libraries worldwide

Effets sublétaux d'une contamination métallique liée à des rejets miniers uranifères sur l'épinoche à trois épines (Gasterosteus aculeatus L.). Implication dans la susceptibilité envers un stress biologique. by Antoine Le guernic( )

1 edition published in 2015 in French and held by 1 WorldCat member library worldwide

Uranium extraction has resulted in a remobilisation of this actinide into mine surrounding ecosystems. Uses of metal salts during mining site rehabilitation, and the natural presence of metals have increased the metal contamination in hydrosystems submitted to mine tailings.In situ experiments were conducted in two former French uranium mining sites. Three-spined stickleback caging was used to determine the sublethal effects of this metal mixture on this freshwater fish, as well as its effects on fish susceptibility to a sudden biological stress.This pollution, characterised by higher metal concentrations (especially for uranium), has led to an oxidative stress in sticklebacks visible through several biomarkers, and other effects dependent on the study site. The polymetallic contamination has modified the stickleback responses to the biological stress, by preventing their phagocytic and antioxidant responses. This work has reinforced the interest of the caging technique during environmental studies and that of immunomarkers in a multi-biomarker approach
Développement de marqueurs d'immunocompétence chez le gastéropode d'eau douce Lymnaea stagnalis et applications en écotoxicologie by Paul Boisseaux( )

1 edition published in 2017 in French and held by 1 WorldCat member library worldwide

Dans l'environnement, les organismes sont soumis à des stress de multiples natures (e.g., polluants et pathogènes/parasites). Dans ce contexte, le système immunitaire joue un rôle fondamental. La pression chimique exercée par les polluants peut impacter leur système immunitaire, affaiblir leur santé et les prédisposer à contracter des maladies infectieuses. En écotoxicologie, l'évaluation de marqueurs d'immunocompétence peut servir à diagnostiquer l'état de santé des organismes et mieux comprendre le danger immunotoxique des xénobiotiques. Lymnaea stagnalis est un gastéropode d'eau douce représentatif de nombreux milieux lentiques sur la planète. Des tests OCDE de reprotoxicité sont disponibles mais peu d'études se sont focalisées sur des paramètres immunotoxiques. Le premier axe de la thèse se porte sur la connaissance et le développement de méthodes pour évaluer l'immunocompétence de manière répétée à l'échelle individuelle chez L. stagnalis. Le deuxième axe évalue cette approche multibiomarqueurs dans différents contextes écotoxicologiques. Plusieurs populations (élevages vs terrain), temps d'expositions (3 vs 29 - 44 jours), concentrations d'exposition (environnementales vs non réalistes) et contaminants ont été évalués : des médicaments (les anti-cancéreux Etoposide et cyclophosphamide, l'anti-rejet de greffes cyclosporine A et l'antiinflammatoire diclofenac), l'insecticide deltaméthrine, le métal cadmium et des effluents de station d'épuration. Des comparaisons entre des traits de vie et des traits immunitaires ont été réalisées. La réflexion est bâtie sur les questionnements relatifs à (i) la sensibilité de l'approche, (ii) le temps de réponse des marqueurs, (iii) leur précocité physiologique, (iv) la transposition d'immunotoxicité de xénobiotiques entre le phylum des chordata et L. stagnalis et (vi) l'utilisation pratique de L. stagnalis (élevages, collecte en milieu naturel, calibration, reproduction)
Etudes des effets de l'eau tritiée sur les stades de développement précoces chez le poisson zèbre (Danio rerio) : caractérisation des modes d'action by Caroline Arcanjo( )

1 edition published in 2018 in French and held by 1 WorldCat member library worldwide

In France, tritium is mainly released as tritiated water (HTO) by nuclear power plants and nuclear reprocessing plants. The developmental and reprotoxic effects of tritium have already been studied. However, few studies focus on molecular effects. In this context, the aim of this thesis is to evaluate the effects of HTO on the embryo-larvae stages of the zebrafish (Danio rerio). Two dose rates, 0.4 and 4 mGy/h, were tested. A protocol for the measurement of activity in organisms was developed. It has (i) confirmed that the internalization of HTO is rapid, (ii) allowed the discrimination of the tissue-free-water-tritum and organically bound tritium forms, and (iii) allowed the calculation of the dose permitting to link the observed effects to a received dose. The effects of HTO were evaluated at different biological levels. At the molecular level, a transcriptomic analysis (mRNAseq) showed the modulation of genes involved in muscle contraction and eye development at 24hpf, as well as in the circadian rythm and the response to oxidative stress at 96hpf. Modulation of genes involved in the DNA damage repair was shown. At higher levels of organization, some alterations of muscle fibers were observed for both dose rates. A decrease in swimming velocity was shown at 96 hpf after exposure to 0.4 mGy/h. This work allowed a better understanding of the tritium internalization, to characterize the absorbed dose in organisms and to better understand the effects of tritium
Metabolic programming of zebrafish, Danio rerio uncovered : physiological performance as explained by Dynamic Energy Budget theory and life-cycle consequences of uranium induced perturbations by Starrlight Augustine( )

1 edition published in 2012 in English and held by 1 WorldCat member library worldwide

The aim of this dissertation is to characterize the toxicity of depleted uranium (U) on the metabolism of zebrafish, Danio rerio. The underlying hypothesis of this work is that effects of U show up as effects on the metabolism of the individual. Consequently, we characterized physiological performance using Dynamic Energy Budget (DEB) theory since it is the only theory which simultaneously specifies ingestion, assimilation, growth, reproduction, maturation, maintenance and ageing over the whole life-cycle at varying food availability. Thus a DEB model was built which quantifies and predicts how the physiological performance of zebrafish relates to food level (and temperature). We showed that development accelerates after birth until metamorphosis after which acceleration ceases. Furthermore, somatic maintenance costs are very high.A module specifying toxico-kinetics of U in a feeding, growing and reproducing individual was incorporated into the DEB model. The model was then applied to toxicity data (from the literature or acquired during this thesis) in order to determine which processes are affected by U. Our results show that, from 0 nM onwards, U increases costs for growth and either increases somatic maintenance or decreases assimilation. We were unable to detect effects on maturation. A histological study showed that U alters histology of the gut wall and may perturb host-microbe homeostasis. By accounting for differences in initial conditions between individuals we were able to explain a number of seemingly contradictory results. The take home message is: observations on individuals should not be averaged for groups of individuals
Pertinence d'une approche multibiomarqueurs en biosurveillance active avec l'épinoche à trois épines (Gasterosteus aculeatus) by Audrey Catteau( )

1 edition published in 2019 in French and held by 1 WorldCat member library worldwide

Many chemical contaminants have been produced and spread into aquatic environments and given rise to anthropogenic activities. In order to assess and predict the impacts of this contamination on organisms and ecosystems, several tools were developed. Among them, biomarkers have been shown to be sensible and early indicators of a perturbation at sub individual level. These physiological responses can be measured in native individuals as well as in individuals from husbandry imported on the field through caging systems. This latter approach, mostly known as active biomonitoring approach, brings with it the ability to overcome some confounding factors present when native individuals are used (history, exposure time, adaptation mechanism). Additionally, the use of imported individuals enables the study of sites in which the model species is endangered or absent. This active methodology also seems more effective in defining reference levels for the comparison of sites over large geographic scales. In this context, the present work aims to assess the relevance of using a new model species in active biomonitoring approach: the three spined stickleback (Gasterosteus aculeatus). Several physiological functions have been chosen to be representative of the global health of individuals (antioxidant system and oxidative damages, biotransformation enzymes, reproduction system, innate immune system, synaptic transmission). As a first step, the effect of a restricted access to food and the confinement induced by caging conditions have been characterized in two seasons with the aim to ensure a correct and thorough interpretation of the results. Then, the approach has been deployed on the field (i) to assess the efficiency of a constructed wetland built downstream a wastewater treatment plant (WWTP), (ii) to assess the potential effects of several effluents of WWTP and (ii) to discriminate different sites along the Meuse basin (Interreg DIADeM program). Findings have been encouraging in highlighting the relevance of the three spined stickleback as a sentinel species and the selected biomarkers in this context of contamination in active biomonitoring. In a larger scale, these same tools have made it possible to discriminate the different sites in the Meuse basin according to the modulated physiological functions. Thus, this work has demonstrated the potential of the multibiomarker approach in active biomonitoring with the three spined stickleback in the assessment and the characterization of water quality
Caractérisations structurale et fonctionnelle des populations hémocytaires de la moule zébrée (Dreissena sp.) en vue de leur utilisation en évaluation du risque écotoxicologique. by Lauris Evariste( )

1 edition published in 2016 in French and held by 1 WorldCat member library worldwide

L'extension des activités humaines est responsable du rejet de molécules et de perturbations climatiques pouvant affecter la physiologie des organismes aquatiques. La moule zébrée possède des caractéristiques biologiques faisant d'elle une espèce intéressante en surveillance environnementale. Chez cet organisme, les hémocytes constituent une cible privilégiée pour la mise en place d'une approche multi-biomarqueurs. En effet, ces cellules à fonctionnalités multiples sont impliquées dans les grandes fonctions physiologiques de l'espèce et la régulation de l'homéostasie des individus. L'objectif de ce travail est de développer les outils analytiques permettant d'étudier les réponses hémocytaires de la moule zébrée. Les expérimentations menées ont permis de caractériser la structure des populations hémocytaires ainsi que leurs fonctionnalités propres en lien avec le processus de phagocytose. L'utilisation de ces biomarqueurs dans divers contextes indique une forte adaptabilité de l'espèce aux conditions environnementales. Les résultats montrent l'intérêt d'analyser les activités hémocytaires à l'échelle des sous populations comparativement à l'approche globale ne tenant pas compte de la diversité cellulaire. Il a été observé que certains facteurs comme le statut reproducteur ou l'espèce échantillonnée (D. polymorpha vs D. bugensis) constituent des facteurs de confusion importants. Il ressort également un positionnement fort du test de phagocytose en tant que marqueur de sensibilité aux contaminants. Ce travail constitue un ensemble de données voué à être utilisé dans des contextes multiples aussi bien en écotoxicologie qu'en écophysiologie
Développement d'outils pour l'évaluation d'une contamination chimique chronique : un enjeu pour la veille environnementale en milieu littoral by Marine Breitwieser( )

1 edition published in 2018 in French and held by 1 WorldCat member library worldwide

The coastline faces chronic chemical contamination due to numerous toxic pollutants (residues of pesticides and medicines, heavy metals, etc.) causing public health issues and environmental degradation. Whereas some contaminants are efficacious at low doses, others lead to dangerous cocktail effects on organisms. The main contaminants are assayed regularly among strategic stages linked to water resource. There is a particular focus on supply and food. Nonetheless, due to the proliferation of contaminants released in the environment, there is no effective monitoring system taking the real extent of the problem into consideration. Moreover, unlike existing methods for humans, there is no finalised or standardised approach to assessing the health of state of aquatic invertebrates, while they represent more than 95% of the biodiversity. The purpose of this thesis work involving interdisciplinary research was to evaluate the pollution impacts on the coastline species (coastal fringe and port areas). A first part of the study aimed at designing effective methods of contamination assessment on bivalves by organic and inorganic pollutants (ecotoxicology). Another part focused on analysing biological effects of pollutants by developing a joint use of several biomarkers (ecophysiology). Thus, like work carried out by public health, this thesis project defined for the first time several analytical and statistical steps on monitoring the state of health of marine organisms and the water quality in coastal areas
 
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Audience level: 0.94 (from 0.78 for Pertinence ... to 0.99 for Etudes des ...)

WorldCat IdentitiesRelated Identities
Alternative Names
Beatrice Gagnaire onderzoeker

Beatrice Gagnaire researcher

Languages
English (10)

French (8)