WorldCat Identities

Rouleux-Bonnin, Florence (1965-....).

Overview
Works: 5 works in 7 publications in 2 languages and 9 library holdings
Roles: Thesis advisor, Author, Contributor
Classifications: QH345, 570
Publication Timeline
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Most widely held works by Florence Rouleux-Bonnin
Exogenous mRNA delivery and bioavailability in gene transfer mediated by piggyBac transposition by Solenne Bire( )

1 edition published in 2013 in English and held by 2 WorldCat member libraries worldwide

Mise au point d'un système dérivé du transposon Mos1 comme vecteur non viral de transfert de gène en cellules eucaryotes by Gwenhael Jegot( Book )

2 editions published in 2007 in French and held by 2 WorldCat member libraries worldwide

The mariner Mos 1 transposon can naturally move into the genome. The first research axis of this work concern the transposon characteristics which act upon the transposition activity of Mos1 (like the nucleic content of the transposon and its size). It have shown that a size of transgene upper than 2,5 kb limit the transposition efficiency, that a strong GC percentage favour the transposition, and that there is no minimal size for Mos1 transposition. The second research axis concern the adaptation and improvement of the Mos1 system to the eukaryotic cells, to developp a nonviral vector for gene transfer. The addition of a nuclear localization sequence improve the nuclear transfer, the "humanization" of the transposase sequence increase it expression in mammal cells, and the use of the HSVtK suicide system permit to limit the recombination events
EXPRESSION DE LA LECTINE MEMBRANAIRE AFFINE DU MANNOSE DES CELLULES DE LA LIGNEE MONOCYTAIRE : MODULATION AU COURS DE LA DIFFERENCIATION by Florence Rouleux-Bonnin( Book )

2 editions published in 1994 in French and held by 2 WorldCat member libraries worldwide

LES MONOCYTES/MACROPHAGES, EN TANT QUE CELLULES CLES DU SYSTEME IMMUNITAIRE, EXERCENT DE NOMBREUSES FONCTIONS DONT LE DECLENCHEMENT MET EN JEU DES RECEPTEURS MEMBRANAIRES. NOUS NOUS SOMMES INTERESSES AUX LECTINES DE LA LIGNEE MONOCYTAIRE, DONT L'EXPRESSION EST ETROITEMENT REGULEE LORS DE LA DIFFERENCIATION ET L'ACTIVATION DES MONOCYTES, ET PLUS PARTICULIEREMENT A LA LECTINE SPECIFIQUE DU MANNOSE DE 175 KDA. NOUS AVONS ETUDIE PAR PCR QUANTITATIVE, L'EXPRESSION DU GENE DE LA LECTINE AU COURS DE LA DIFFERENCIATION. NOUS AVONS MONTRE QUE L'ARN MESSAGER N'EST PAS DETECTABLE DANS LES MONOCYTES FRAICHEMENT ISOLES. PAR CONTRE, L'EXPRESSION DU GENE AUGMENTE TRES RAPIDEMENT LORSQUE LES MONOCYTES SONT CULTIVES PENDANT 24 HEURES PUIS DIMINUE POUR SE STABILISER A UN NIVEAU DE BASE AU BOUT DE 96 HEURES. IL SEMBLE DONC QUE L'INDUCTION DE LA TRANSCRIPTION DU GENE SOIT UN EVENEMENT PRECOCE. NOUS AVONS COMPARE L'EXPRESSION DU GENE DE LA LECTINE SPECIFIQUE DU MANNOSE A L'EXPRESSION DU GENE DE LA LECTINE SPECIFIQUE DU GALACTOSE DE 30 KDA. LES RESULTATS MONTRENT QUE L'EXPRESSION DE CETTE DERNIERE AUGMENTE GRADUELLEMENT EN FONCTION DU TEMPS DE CULTURE. LES MECANISMES QUI SOUS-TENDENT LA REGULATION DE L'EXPRESSION DES DEUX LECTINES SONT DONC DIFFERENTS. UNE SEQUENCE D'ENVIRON 400 PB, EN AMONT DU GENE A ETE ISOLEE. ELLE ASSURE L'EXPRESSION D'UN GENE REPORTEUR DANS LES CELLULES DE TYPE MACROPHAGE. CECI DEMONTRE QUE LA SEQUENCE CLONEE CONTIENT LE PROMOTEUR DU GENE DE LA LECTINE ET CONTIENT UNE SEQUENCE DE CONTROLE NEGATIF DE LA TRANSCRIPTION QUI A TOUTES LES CARACTERISTIQUES D'UN SILENCEUR. CELUI-CI EST RESPONSABLE DE L'EXPRESSION TISSULAIRE SPECIFIQUE DU GENE DE LA LECTINE. CES RESULTATS MONTRENT QUE L'EXPRESSION DU GENE DE LA LECTINE EST ETROITEMENT REGULEE AU COURS DE LA DIFFERENCIATION ET QUE CETTE REGULATION INTERVIENT, ENTRE AUTRES, PAR L'INTERMEDIAIRE D'UN SILENCEUR. IL RESTE A DEFINIR QUELLES SONT LES MODALITES D'ACTION DE CETTE SEQUENCE ET DES AUTRES SEQUENCES CONSENSUS TROUVEES DANS LE FRAGMENT CLONE DANS LA REGULATION DE L'EXPRESSION
Factors acting on Mos1 transposition efficiency by Ludivine Sinzelle( )

1 edition published in 2008 in English and held by 2 WorldCat member libraries worldwide

Optimisation de la biosécurité du vecteur transposon piggyBac pour le transfert de gène : utilisation des ARN messagers et des insulateurs. by Solenne Bire( )

1 edition published in 2011 in French and held by 1 WorldCat member library worldwide

Advances in biotechnology have enabled the development of tools for gene transfer applicable to transgenesis, bioproduction and gene therapy. But, 3 major challenges must be met to ensure a secure system: the safety and effectiveness of the transfer. the targeted and controlled integration into the genome. and the level of transgene expression over time. In this aim, my thesis project was to validate solutions to improve the biosafety of the piggyBac transposon, which requires a plasmid carrying the gene of interest to be inserted in the genome, and a source of transposase which catalyzes the transgene integration. One approach of my thesis work is to deliver the source of piggyBac transposase as an mRNA molecule instead of DNA. This strategy aims to improve the stability of the integration and reduce the genotoxic effects by limiting the transposase in the cells. For the 1st time, the bioavailability of the transposase rnRNA and the optimal conditions for its use in human cells were determined to increase the biosafety of the transposon system. The 2nd objective ofmy project is to improve the expression of the transgene by adding insulators known to counteract the transgene silencing. This strategy reduces the number of integrations required ta get a sufficient expression of the transgene and thus, improve biosecurity. Two candidates have been identified to improve transgene expression. The combination of the mRNA and insulator strategies is promising to secure the piggyBac-mediated gene transfer and to maintain the expression of the gene of interest
 
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Alternative Names
Bonnin, Florence

Bonnin-Rouleux, Florence

Rouleux, Florence

Languages