WorldCat Identities

Studer, Vincent (19..-....; directeur de recherche)

Overview
Works: 9 works in 10 publications in 2 languages and 12 library holdings
Roles: Other, Author, Thesis advisor, Opponent
Publication Timeline
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Most widely held works by Vincent Studer
Pompage électrocinétique et autres éléments intégrables pour la microfluidique by Vincent Studer( Book )

2 editions published in 2004 in French and held by 3 WorldCat member libraries worldwide

Subrepellent doses of Slit1 promote Netrin-1 chemotactic responses in subsets of axons by Isabelle Dupin( )

1 edition published in 2015 in English and held by 2 WorldCat member libraries worldwide

Conception and fabrication of reusable microfluidic tools to study the dynamics of biological phenomena : application to antibiotic influx/efflux in bacteria and to cell migration during mouse development by Xuan Zhao( )

1 edition published in 2017 in English and held by 1 WorldCat member library worldwide

We want to analyze the responses of biological systems to the introduction of perturbations and spatio-temporal modulations. More specifically, in order to develop innovative strategies for the study of biological systems, we propose to use microfluidic tools. We design adapted microsystems that can locally influence biological behaviors, so that a macroscopic experimenter can control the external environment of biological objects whose scale is microscopic. This engineering strategy is generic and multidisciplinary. In this thesis, it has been implemented in two collaborative projects, on one hand, on the scale of the E.coli bacterium and on the other hand on that of the embryo of mouse at an early stage post-implantation. The selected study objects are characteristic in many respects of the biological fields concerned: size, representativeness, complexity.We extended our expertise in fluidic device design and manufacturing to the service of the DISCO beamline of the synchrotron SOLEIL and the IRIBHM mouse embryology team. The key point of my work has been to design and manufacture reusable microfluidic tools for generic research, which allow biologists to dispense with the use of a clean room.More precisely, the project of microbiology at SOLEIL had for object the study of the influx and the efflux of antibiotic molecules in bacteria. To do this, we have developed a reusable device for immobilizing microorganisms and changing their chemical environment during UV imaging on epifluorescence microscopy. This study is carried out using two typical partners: the Escherichia coli bacterium and a drug from the fluoroquinolone family. The embryology project relied on the localized electroporation of nucleic acids within mouse embryos and the monitoring of cellular migrations.In this thesis, we have developed not only reusable micro-devices but also experimental protocols adapted to the use of these miniaturized instruments.More precisely, the microbiology project at SOLEIL focused on the influx and the efflux of antibiWe have developed a reusable device for immobilizing those microorganisms and changing their chemical environmentduring UV imaging on an epifluorescence microscopy. This study was carried out using two typical partners: thebacterium and a drug from the fluoroquinolone family. The embryology project relied on the localized electroporation ofnucleic acids into mouse embryos and the monitoring of cell migrations
Cytométrie par imagerie grand champ en phase et fluorescence : applications en hématologie by Isaure Le Cardinal de Kernier( )

1 edition published in 2019 in English and held by 1 WorldCat member library worldwide

Blood cell population analyses allow detecting a wide scope of clinical disorders, ranging from anemias to malaria. A very large number of cells ought to be considered so as to ensure the statistical significance of the result, and in turn, yield a reliable diagnosis. Currently, hematology analyses are based on flow cytometry techniques. High throughput is obtained at the expense of the information content of each acquisition. To reduce the time-to-result, and to minimize the complexity and cost of the systems dedicated to analyzing cell populations, the current need is to reduce the number of acquisitions and optimize the information content. This thesis focuses on single-shot image cytometry as an alternative to flow-based cytometry. It aims at obtaining a set-up based on optical contrasts for the study of large cell populations while preserving the ability to resolve individual cells. We investigate a multi-scale and multi-modal approach to detect, characterize, and classify blood cells. To evaluate the feasibility and clinical relevance of the method, we developed two proof-of-concept set-ups, respectively called the mesoscope and the miniscope. The mesoscope, based on optical developments, combines phase contrast with fluorescence. The complementarity of morphological features and the expression of specific fluorophores enables us to accurately classify blood cells, and for example assess Plasmodium falciparum parasitemia in whole blood samples. The results are benchmarked to reference techniques. However, to address the need for point of care analyses, the system should be miniaturized. Hence, we designed the miniscope, a chip-based bimodal imager
Développement de microtechnologies pour l'étude du guidage axonal by Yohan Lecomte( )

1 edition published in 2019 in French and held by 1 WorldCat member library worldwide

Axonal guidance is a very important process during brain development, allowing to give it its structure and organization. The neuroscience scientific community has a growing interest in it during the last years. Several tools belonging to the field of microtechnologies, microfluidics and micropatterning are of important help to study axonal guidance in vitro. They allow to confine neurons and their axons and to apply gradients of guidance molecules. During this thesis, my goal was to develop a system to study the effect of guidance molecules gradients on axonal guidance. For that, I tested several configurations of microfluidic devices, micropatterns and combinations of both.First, we used two approaches to isolate dissociated neurons axons from their somas. Our goal was to study the effect of the molecular environment on neurons growth cones, with a high throughput. The first approach consisted in growing neurons on different proteins patterns. It also allowed to show their capacity to adhere on these patterns. The second one consisted in seeding neurons in a microfluidic device in which, during their growth, axons are separated from somas by microchannels. Then we studied the effect, on the axons, of guidance molecules gradients. To begin, we measured the effect of two guidance molecules: ephrin and semaphorin, by culturing neurons in the presence of patterned gradients of these two molecules. After that, we studied another model where neurons are closer from their environment in vivo, explants growing on laminin patterns containing a gradient. To help the explant positioning, we polymerized hydrogels. Then, we put explants next to patterned gradients of ephrin. Finally, we tried to obtain a soluble gradient of guidance molecules, over a long period of time (days), closer to existing gradients in vivo. In that goal, we wanted to build a microfluidic device enabling the application of a soluble gradient of guidance molecules on neurons. To obtain a constant gradient, we also cultured neurons next to cells expressing netrin, another guidance molecule. Finally, we cultured dissociated neurons and glial cells to study their interactions.All these experiments did not allow to obtain a reliable device to study the effect of molecules on axons growth and guidance. Nevertheless, the configuration consisting in a coculture of neurons next to cells releasing netrin allows us to obtain promising preliminary results. We thus drew up a group of methods that will enable us to finalize the development of a system to study axonal guidance, functional and efficient
Echantillonnage compressif appliqué à la microscopie de fluorescence et à la microscopie de super résolution by Makhlad Chahid( )

1 edition published in 2014 in French and held by 1 WorldCat member library worldwide

My PhD work deals with the application of Compressed Sensing (or CompressiveSampling, CS) in fluorescence microscopy as a powerful toolkit for fundamental biologicalresearch. The recent mathematical theory of CS has demonstrated that, for aparticular type of signal, called sparse, it is possible to reduce the sampling frequencyto rates well below that which the sampling theorem classically requires. Its centralresult states it is possible to losslessly reconstruct a signal from highly incompleteand/or inaccurate measurements if the original signal possesses a sparse representation.We developed a unique experimental approach of a CS implementation in fluorescencemicroscopy, where most signals are naturally sparse. Our CS microscopecombines dynamic structured wide-field illumination with fast and sensitive singlepointfluorescence detection. In this scheme, the compression is directly integratedin the measurement process. Additionally, we showed that introducing extra dimensions(2D+color) results in extreme redundancy that is fully exploited by CS to greatlyincrease compression ratios.The second purpose of this thesis is another appealing application of CS forsuper-resolution microscopy using single molecule localization techniques (e.g.PALM/STORM). This new powerful tool has allowed to break the diffraction barrierdown to nanometric resolutions. We explored the possibility of using CS to drasticallyreduce acquisition and processing times
Micro-impression d'anticorps pour des applications diagnostics et thérapeutiques en immunologie clinique by Geoffrey Delhaye( )

1 edition published in 2021 in French and held by 1 WorldCat member library worldwide

Nous avons développé dans ce travail de thèse un prototype original et innovant dont les applications potentielles dans le domaine de l'immunologie clinique pourraient permettre de répondre à la demande de nouvelles technologies de quantifier de façon fine les fonctions immunitaires de patients. La technique implique l'impression par LIMAP (Light Induced Molecular Adsorption of Proteins) d'anticorps qui ciblent spécifiquement des protéines exprimées par les cellules, et la détection des résultats par RICM (Reflection Interference Contrast Microscopy). Pour valider notre concept, nous avons réalisé un test d'identification cellulaire à partir d'une double impression d'anticorps à l'échelle de la cellule unique qui permet de discriminer des lymphocytes T mémoires primaires humains activés et non-activés. A partir de ces résultats, nous avons effectué un test de suivi de la cinétique d'activation en initiant sur un premier motif imprimé le processus d'activation de lymphocytes T mémoires que l'on observe sur un deuxième motif qui révèle en temps réel une protéine exprimée par les cellules activées.Les travaux que nous avons présentés ont permis de mettre en avant deux avantages indéniables de notre prototype, à savoir sa rapidité en matière de disponibilité des résultats des tests et le fait qu'il ne nécessite pas l'utilisation de marqueurs fluorescents. La combinaison de la technologie LIMAP, qui offre une grande flexibilité d'impression de motifs et d'adsorption de protéines, et de la technologie RICM dont la résolution permet une lecture fine des événements cellulaires ouvre un grand champ d'investigations scientifiques et d'applications dans le domaine médical
Tailoring common hydrogels into 3D cell culture templates. by Aurélien Pasturel( )

1 edition published in 2019 in English and held by 1 WorldCat member library worldwide

L'ingénierie d'hydrogels ; leur structuration et fonctionnalisation à l'échelle cellulaire, est une étape clé pour aboutir à de modèles in-vitro plus physiologiques. À ce jour, elle reste difficile car ces matériaux polymères, mous et riches en eau, sont souvent trop fragiles pour la micro-fabrication traditionnelle. Pour pallier à ce fait, nous avons combinée illumination ultraviolette structurée et chambres de réaction perméables au gaz nous offrant la maitrise sur la distribution de photons, les réactifs et les gaz présents à chaque instant et en tout point d'un champ d'illumination. Nous pouvons ainsi contrôler une photochimie adaptée aux hydrogels les plus répandus et structurer, décorer ou liquéfier ces matériaux. Ensemble ces trois opérations forment une boite à outil complète adaptée aux substrats les plus communs que sont Matrigel, Agar, Poly(acrylamide) et Poly(éthylène-glycol). Nous avons par la suite fabriqué des micro-niches en hydrogel permettant la culture standardisée de lignées cellulaire et de neurones primaires soit par adhésion sur des topographies ou par auto-organisation en sphéroïdes. Ceci démontre que la plateforme est à même de répondre à des enjeux importants de culture cellulaire tridimensionnelle
Etude de l'influence de la topographie du microenvironnement sur la migration des interneurones corticaux par l'utilisation de substrats microstructurés by Claire Leclech( )

1 edition published in 2018 in French and held by 1 WorldCat member library worldwide

In the developing brain, cortical interneurons undergo a long distance migration to reach the cortex where they integrate into cortical networks and regulate their activity in the adult. Different chemical factors have been involved in the guidance of these cells, but the influence of the physical parameters of the environment in which they navigate remains unclear. It has been shown that topographical cues are able to influence and guide the migration of several cell types, a process called contact guidance. This work therefore aimed at testing and understanding the influence of the topography of the environment in the migration of cortical interneurons. By using an experimental system of microstructured substrates, we demonstrated for the first time the existence of contact guidance for these cells. By testing two types of micron-sized pillars, we showed that a change in the shape of the structures could greatly impact cell orientation, morphology, cytoskeleton organization and dynamic behavior. In particular, most interneurons migrating in between square pillars adopt an elongated, unbranched morphology associated with a slow and directed movement, whereas the majority of cells among round pillars exhibit a short and branched morphology associated with a dynamic but wandering movement. Overall, we show that micron-sized topography provides global spatial constraints promoting the establishment of different morphological and migratory states in vitro, highlighting the potential importance of these types of cues in vivo
 
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Audience level: 0.97 (from 0.95 for Subrepelle ... to 1.00 for Etude de l ...)

Alternative Names
Vincent Studer researcher

Vincent Studer wetenschapper

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