WorldCat Identities

Roussel, Mikael

Overview
Works: 18 works in 19 publications in 2 languages and 26 library holdings
Roles: Author, Thesis advisor, Other, Opponent
Publication Timeline
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Most widely held works by Mikael Roussel
Rôle de la cycline D1 dans la physiopathologie du lymphome à cellules du manteau et du myélome multiple by Mikaël Roussel( Book )

2 editions published in 2007 in French and held by 2 WorldCat member libraries worldwide

La cycline D1 est une protéine impliquée dans la régulation du cycle cellulaire et la transcription. Elle est exprimée dans certaines hémopathies lymphoïdes B : myélome multiple (MM), lymphome à cellule du manteau (MCL) et leucémie à tricholeucocytes (HCL) alors que les lymphocytes B ne l'expriment pas physiologiquement. La cycline D1 est codée par CCND1 et transcrite par épissage alternatif en deux formes (a et b). Les protéines cycline D1a et b diffèrent au niveau d'une région impliquée dans la dégradation des protéines et la localisation subcellulaire. Nous avons étudié l'expression des transcrits et protéines de la cycline D1 dans trois hémopathies B (MM, MCL et HCL). La cycline D1b est principalement présente dans le MCL et rarement dans le MM. Le taux de transcrit a est supérieur au taux de transcrit b. Les deux isoformes ont des demi-vies comparables et sont présentes dans le noyau et le cytoplasme. La cycline D1b ne possède pas les propriétés (stabilité, localisation) associée à son potentiel oncogénique. Elle ne semble donc pas jouer un rôle prépondérant dans la physiopathologie du MM et du MCL. Afin de caractériser le mécanisme d'action de la cycline D1a, nous avons transduit cette protéine dans des lignées cellulaires de MM et de lymphomes n'exprimant pas de cycline D1 endogène et représentant des niveaux de différenciation différents. La transduction de la protéine exogène entraîne une augmentation de la prolifération dans des lignées lymphoïdes pro-B (NALM-6, 697), et un blocage du cycle cellulaire dans des lignées B (RAMOS) et de MM (OPM2, LP1). Ce dernier effet est observé après transduction des cellules par une protéine cycline D1 mutée (K112E). Ceci est en faveur d'un effet indépendant de la phosphorylation de pRb. Cet effet pourrait passer par l'activation de la liaison à l'ADN des sous-unités p65 et c-Rel de NF-kappaB. Parmi les perspectives de ce travail, il faudra rechercher de nouveaux partenaires ou cibles transcriptionnelles de la cycline D1
Regulatory myeloid cells: an underexplored continent in B-cell lymphomas by Mikael Roussel( )

1 edition published in 2017 in English and held by 2 WorldCat member libraries worldwide

Mass cytometry defines distinct immune profile in germinal center B-cell lymphomas by Mikael Roussel( )

1 edition published in 2020 in English and held by 2 WorldCat member libraries worldwide

Deciphering myeloid-derived suppressor cells: isolation and markers in humans, mice and non-human primates by Luca Cassetta( )

1 edition published in 2019 in English and held by 2 WorldCat member libraries worldwide

CD16-positive circulating monocytes and fibrotic manifestations of systemic sclerosis by Alain Lescoat( )

1 edition published in 2017 in English and held by 2 WorldCat member libraries worldwide

Multicenter validation of the flow measurement of classical monocyte fraction for chronic myelomonocytic leukemia diagnosis by on behalf of the Groupe Francophone des Myélodysplasies (GFM)( )

1 edition published in 2018 in English and held by 2 WorldCat member libraries worldwide

Résistance à l' apoptose et régulation du cycle cellulaire dans le lymphome splénique à lymphocytes villeux (SLVL) by Mikaël Roussel( Book )

1 edition published in 2002 in French and held by 2 WorldCat member libraries worldwide

Mass cytometry deep phenotyping of human mononuclear phagocytes and myeloid-derived suppressor cells from human blood and bone marrow( )

1 edition published in 2017 in English and held by 1 WorldCat member library worldwide

Recherche d'un marqueur prédictif de sepsis chez une population de réanimation médicale adulte : analyse des données des monocytes obtenues par DxH 800 (Beckman Coulter®) by Edouard Garrot( Book )

1 edition published in 2018 in French and held by 1 WorldCat member library worldwide

Sepsis is defined as life-threatening organ dysfunction caused by a deregulated host response to infection. In intensive care units (ICU), sepsis can be hard to diagnose and is still associated with mortality greater than 10%. Thus the diagnosis of sepsis should be performed as early as possible. Recently the standard deviation of monocyte volume, reflecting the size distribution of circulating monocytes, measured by hematology analyzers (DxH 800, Beckman Coulter) has provided a significant added value to WBC count for the detection of sepsis in an Emergency Department population (Elliott et al, 2017). The main objective of our study was to evaluate the diagnostic accuracy of monocyte parameters, including SD-V-Mo, to discriminate septic from non-septic patients in ICU. Then, we aimed to evaluate if these monocyte parameters were able to specify the microbial etiology of the sepsis. A retrospective cohort study was conducted in ICU at Rennes academic hospital on 969 data of patients hospitalized between October 2015 and January 2018. Diagnosis of sepsis was based on standard definitions. Two monocyte parameters were evaluated: mean- (MN-V-Mo) and standard deviation- (SD-V-Mo) monocyte volume. These parameters were measured on routine CBC testing on a DxH 800 analyzer at the time of ICU admission and normalized with cell control 6C provided by the supplier. Among 969 data of patients admitted during the observation period, 40 % had sepsis (N = 392). Septic and non-septic patients had a median age of 63 [50-72] and 62 [49-69] years, respectively. The MN-V-Mo and the SD-V-Mo were significantly increased at admission for septic patients (p< 0.0001). ROC curves analyses evidenced Se and Sp 71 % (AUC 0,76) for a MN-V-Mo ratio threshold at 0,85 Se and Sp 74 % (AUC 0,81) for SD-V-Mo ratio threshold at 0,74. Both parameters were significantly different between control group and sepsis of bacterial origin and viral origin but did not allow to differentiate the two etiologies. SD-V-Mo could also discriminate control group and sepsis without microbial documentation. The incorporation of MN-V-Mo and SD-V-Mo obtained with CBC were shown to improve detection of sepsis at the time of admission in ICU. Leukocyte cell population data are obtained automatically, routinely, within minutes, requiring neither additional sampling nor additional cost to that of CBC thus making their prospects very promising. Hopefully, these parameters can ultimately be incorporated into a decision rule for the screening of sepsis samples at admission in ICU
Dialogue tumeur microenvironnement dans le lymphome folliculaire : interaction BCR / DC-SIGN by Steve-Alexandre Genebrier( )

1 edition published in 2020 in French and held by 1 WorldCat member library worldwide

Le lymphome folliculaire (FL) est une prolifération lymphocytaire B maligne très dépendante de son microenvironnement. De nombreux mécanismes de soutien de la tumeur par son microenvironnement ont ainsi été décrits. En particulier le récepteur à l'antigène (BCR) des cellules tumorales est capable, via des modifications génétiques induisant l'introduction de résidus mannoses, d'interagir avec la lectine DC-SIGN exprimée par les macrophages associés aux tumeurs (TAM) : cette interaction délivre un signal d'activation et de survie aux cellules lymphomateuses. Nous formulons l'hypothèse que cette interaction a aussi des conséquences sur les cellules myéloïdes DC-SIGNpos et qu'elle module leur phénotype pour favoriser la croissance tumorale. Nous avons mis au point un panel de cytométrie de masse (cytometry by time-of-flight, CyTOF) pour caractériser l'hétérogénéité des cellules ganglionnaires exprimant DC-SIGN. De plus, pour pallier la difficulté d'isoler des TAM de FL, nous avons évalué la pertinence de différents types de cellules myéloïdes obtenues in vitro comme modèles de TAM. Nous avons montré, en transcriptomique et en CyTOF, la supériorité des macrophages obtenus par polarisation in vitro de monocytes en présence d'IL-4 (M-IL4 et M-FL), une cytokine surexprimée dans le FL, par rapport à la lignée monoblastique U937 DC-SIGN à représenter un modèle valide de TAM de FL. L'interaction BCR/DC-SIGN a été reproduite par des cocultures entre des cellules DC-SIGNpos (U937 DC-SIGN ou M-IL4) et des lymphocytes B exprimant un BCR mannosylé (lignée L3055 ou lymphocytes B de FL). Cette interaction n'entraîne pas d'internalisation de DC-SIGN. Le signal délivré aux cellules myéloïdes reste donc à caractériser en évaluant d'autres aspects de l'activation de DC-SIGN
Les nouvelles approches de l'analyse multi-paramétrique en cytométrie de masse : caractérisation des cellules réservoirs du VIH by Aurélien Corneau( )

1 edition published in 2018 in French and held by 1 WorldCat member library worldwide

Mass cytometry (CMM) has revolutionized the study of cell and phenotypic diversity, significantly increasing the number of markers that can be analyzed simultaneously (41 to date). By making it possible to precisely define the state of the lymphocyte populations, particularly regarding their differentiation, activation and entry into the cell cycle, the CMM has revealed small subsets so far unknown. In this study, the CMM was used to try to better characterize the HIV's reservoirs. With the introduction in 1996 of Combined Antiretroviral Therapy (ART), HIV infection has shifted from a fatal destiny to a manageable chronic disease with a normal life span through a reduction in active viral replication (the amount of virus is below optimal detection limits). However, if treatment is interrupted, the viral load increases again in the patient due to viable provirus reservoirs located in long-lived cell populations that cannot be eliminated by current treatments. These reservoirs constitute a major obstacle to the eradication of HIV. The best characterized reservoir is that of CD4+ T cells and is mainly hosted in TCM, TTM, TSCM and Tfh. A first study allowed us to evaluate the stages of the cell cycle in association with markers of differentiation, activation and exhaustion, leading to a thorough assessment of the quiescent state of CD4 T cells likely to harbour latent reservoirs of HIV. This broad multiplex analysis demonstrates that some subsets of LTCD4+CD25-HLA-DR- classically considered "at rest" - do actually contain significant amounts of cells in cycling or expressing inhibitory receptors, opening new pathways for redefining CD4 T quiescent cells from peripheral blood. A second study aimed to define CD4+ T Cells populations producing HIV in vivo. We have developed a multiparametric analysis on cells of HIV+ patients under ART and in therapeutic interruption phase (ATI). This study shows that CD3+CD4+CD32high cells express a high level of activation markers and receive important activation signals via cytokines, unlike CD32a- cells. On the other hand, the analysis of HIV-producing LTCD4+ (expressing the p24 capsid protein), allowed us to detect a very small number of p24+ positive cells (less than 0.004% in ATI phase but none before). The phenotype of the producing cells was then highlighted. These are T lymphocytes that do not express CD8, enriched with a factor 4 in TSCM cells, and a factor 2 in TFH. These populations are highly enriched in activated cells co-expressing 3 activation markers (increased by a factor of 20) and are in cycle (Ki67+) and/or over-express immune control molecules (ICPs) with an enrichment of a factor of 500. This allows us to detect producing cells with much higher frequencies in these TCD3+CD8- populations in cycles up to 0.08%, and in G2 phase (2.46%), but also in cells with poly-expression of 4 immune-checkpoints (2.27%). The advent of mass cytometry has exponentially increased the information we could get on a cell. Thanks to this tool, cell cycle identification, in correlation with different phenotypic markers, makes possible the exploration of previously inaccessible information, including the analysis of latent and productive reservoirs of HIV. This work enables us to characterize as precisely as possible these HIV-producing cells, but also the latent cells, and potentially reservoirs of the virus
Relevance physiopathologique des productions cytokiniques dans la Leucémie Lymphoïde Chronique by Maissa Mhibik( )

1 edition published in 2018 in French and held by 1 WorldCat member library worldwide

Intérêt dans le suivi de greffe rénale de la formule leucocytaire par cytométrie en flux by Mathilde Huez-Rocher( Book )

in French and held by 1 WorldCat member library worldwide

Evaluation du kit FMH quikquant pour la quantification de l'hémorragie fœto-maternelle et des hématies contenant un fort taux d'hémoglobine fœtale (F cells) by Cédric Pastoret( Book )

1 edition published in 2013 in French and held by 1 WorldCat member library worldwide

The detection of fetomaternal hemorrage (FMH) is a crucial test for the obstetrical management of Rhesus D-negative women. Indeed, the amount of fetal erythrocytes in maternal blood samples determines the dose of anti-D immunoglobulin to prevent allo-immunization. The Kleihauer-Betke test (KBT) is the most widely used assay, althought it is not reproductible and labour extensive. In the current study, we sought to evaluate the performance of a flow cytometry (FCM) kit, FMH QuikQuant, using an anti-Hemoglobin F antibody. Blood samples from 83 pregnant women and 58 ombilical cord blood dilutions in adult blood were tested in parallel by KBT and FCM. Firstly, FCM showed a good correlation with KBT, a higher accuracy and reproducibility than the standard method. Moreover, we reported a systematic over-estimation with KBT, probably leading to inappropriate treatments. Finally, F-cells (maternal erythrocytes with high amount of HbF) could be a specific marker of myelodysplastic syndromes
Evaluation en cytométrie en flux de la phosphorylation de STAT5 et STAT3 en réponse respectivement à la stimulation du BCR et à l'interleukine-21 dans les ganglions de lymphome folliculaire par rapport à des ganglions non tumoraux by Eric Guiheneuf( )

1 edition published in 2011 in French and held by 1 WorldCat member library worldwide

Le lymphome folliculaire (LF) est un lymphome dérrvant des lymphocytes B du centre germinatif (LyBCG) et les cellules tumorales possèdent un phénotype proche de celui des LyBCG. Dans le centre germanitif, la prolifération et la survie des LyBCG sont sous la dépendance des signaux provenant du microenvironnement notamment du lymphocyte T auxiliaire folliculaire (LyTFH). Les LyTFH produisent l'interleukine 21 (IL-21) indispensable au maintien du centre germanitif et à la différenciation terminale des LyB. dans ce travail nous avons analysé par une technique de cytométrie en flux la phosphorylation des protéines STAT3 et STAT5 en réponse aux stimulations par l'IL-21 et du BCR de cellules de biopsies de LF. Nos résultats montrent une modification des niveaux de phosphorylations par rapport à des cellules de ganglions et d'amygdales non tumoraux. Nous observons une augmentation de la phosphorylation des STAT3 dans les LyB et les LyTFH de LF. Ces résultats sont en faveur d'un lien entre les cellules tumorales et les LyTFH infiltrant la tumeur; le LyTFH pourrait via l'IL-21 avoir un rôle dans la survie et la prolifération des cellules tumorales comme celui qu'il exerce avec les LyBCG
Hétérogénéité des sous-populations monocytaires dans le sang d'hémopathies lymphoïdes B chroniques by Anna Mingam( )

1 edition published in 2015 in French and held by 1 WorldCat member library worldwide

In many neoplastic or inflammatory contexts, variations in the repartition of circulating monocytes subpopulations have been noted. We studied their repartition and their phenotypic changes in the blood of the four most frequent chronic lymphopathy B (follicular lymphoma, chronic lymphocytic leukemia, lymphoma of the marginal zone and mantle cell lymphoma) in order to characterize the impact of the lymphoma on monocytes. In all cases, the absolute value of circulating monocytes increases. The subpopulations impacted are quite heterogeneous, depending on the lymphoma. However, intermediary monocytes CD14+CD16+ and a section of the non-classical monocytes expressing the marker Slan CD14dimCD16+Slan+ increase consistently. On the other hand, only intermediary monocytes CD14+CD16+ are positively correlated to the lymphomatous infiltration. Theses results mirror the determining role of monocytes in the tumorous environment
 
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Alternative Names
Mikael Roussel wetenschapper

Languages
French (12)

English (7)