WorldCat Identities

Sanni, Ambaliou

Overview
Works: 23 works in 25 publications in 2 languages and 41 library holdings
Roles: Contributor, Thesis advisor, Other, Opponent, Author
Publication Timeline
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Most widely held works by Ambaliou Sanni
Récepteurs Toll (TLRs) et leurs voies de signalisation : rôles dans les réponses innée et acquise aux antigènes de la tuberculose et du paludisme (malaria) by Dieudonnée Togbe Togbe Gbetchede( )

2 editions published in 2007 in French and held by 3 WorldCat member libraries worldwide

Les maladies infectieuses sont responsables de 14 millions de décès chaque année dans le monde. La reconnaissance des motifs structuraux des pathogènes se fait par l'intermédiaire de récepteurs dont les "Toll-like" ou TLRs. Les TLRs et leurs molécules adaptatrices sont des composants fondamentaux de l'immunité innée. Cette étude vise à comprendre le rôle des TLRs, de leurs voies de signalisation et de certaines cytokines induites dans les réponses immunitaires mises en œuvre dans les modèles de maladies infectieuses comme la malaria cérébrale, la tuberculose, l'asthme allergique et non allergique. L'analyse est réalisée chez les souris génétiquement déficientes pour différents TLRs, les molécules adaptatrices MyD88, TIRAP, et TRIF ainsi que les molécules de la famille du TNF. Cette étude a permis de montrer que la voie de signalisation impliquant TLR4, MyD88 et TIRAP est essentielle, alors que les voies impliquant TRIF ou IL-1R1 et IL-18R ne sont pas indispensables à l'inflammation des voies respiratoires induite par le LPS. Dans le modèle d'infection par Plasmodium berghei Anka, ni les TLRs, MyD88, TIRAP, ou TRIF ne sont impliqués dans la pathogenèse de la malaria cérébrale. Nous avons également mis en évidence le rôle majeur joué par LTa, LTb et les voies de signalisation impliquant TNFR2 et LTbR dans la malaria cérébrale. Finalement, dans le modèle d'infection à Mycobactérium tuberculosis, au-delà des voies TLRs, la voie IL-1R1 dépendante de MyD88 est essentielle pour le contrôle de l'infection aigue
Is a Plasmodium lactate dehydrogenase (pLDH) enzyme-linked immunosorbent (ELISA)-based assay a valid tool for detecting risky malaria blood donations in Africa? by Pascal S Atchade( )

1 edition published in 2013 in English and held by 2 WorldCat member libraries worldwide

Climate variability and status of the production and diversity of sorghum (Sorghum bicolor (L.) Moench) in the arid zone of northwest Benin by Innocent Dossou-Aminon( )

1 edition published in 2015 in English and held by 2 WorldCat member libraries worldwide

Combined effects of okadaic acid and cadmium on lipid peroxidation and DNA bases modifications (m5dC and 8-(OH)-dG) in Caco-2 cells by Adama Traoré( )

1 edition published in 2000 in English and held by 2 WorldCat member libraries worldwide

Exhaled carbon monoxide: a non-invasive biomarker of short-term exposure to outdoor air pollution by Herve Lawin( )

1 edition published in 2017 in English and held by 2 WorldCat member libraries worldwide

Triterpenoids from Acacia ataxacantha DC: antimicrobial and antioxidant activities by Abdou Madjid O Amoussa( )

1 edition published in 2016 in English and held by 2 WorldCat member libraries worldwide

Plasmodium falciparum merozoite surface antigen-specific cytophilic IgG and control of malaria infection in a Beninese birth cohort by Rafiou Adamou( )

1 edition published in 2019 in English and held by 2 WorldCat member libraries worldwide

Acthaside: a new chromone derivative from Acacia ataxacantha and its biological activities by Abdou Madjid O Amoussa( )

1 edition published in 2016 in English and held by 2 WorldCat member libraries worldwide

The hide and seek of Plasmodium vivax in West Africa: report from a large-scale study in Beninese asymptomatic subjects by Philippe Poirier( )

1 edition published in 2016 in English and held by 2 WorldCat member libraries worldwide

Contribution à l'étude de l'ARN polymérase II de Plasmodium falciparum by Adonis Hazoumé( Book )

2 editions published in 2008 in French and held by 2 WorldCat member libraries worldwide

The parasite Plasmodium falciparum is the causative agent of the most burdensome form of human malaria. His RNA polymerase II (RNAPII) is responsible for transcription of protein coding genes. The sequencing of the full genome of the parasite enabled us to recover a complete set of genes sequences encoding the putative RNAPII subunits of the parasite. We have cloned those subunits by genetic reconstitution. We investigated the functional conservation of the Plasmodium RNAPII subunits using a genetic test in the yeast Saccharomyces cerevisiae. The subunits PfRPB4-5-7-9 and 12 can complement defective yeast mutants. Those results demonstrate that those subunits are orthologs of yeast conterparts. We establish then some stable yeast strains expressing Plasmodium proteins. No interspecific complementation was observed for the subunits PfRPB3-6-8-10-11. We construct viable yeast strain where the residus implicated in the interaction of the mushrom toxin alpha-amanitin where substituted by their homologs in Plasmodium and human. We screen those strains with a chemical compounds library. We don't find any drug enable to distinguish the modified strains from wild-type one. But this screen, consisting in a simple cellular test and easy to perform, could permit to identify drugs that inhibit the transcriptional activity of the parasite enzyme. Those chemical compounds will the fist step towards the discovery of a potential new class of antimalarials drugs. In this work, we also study the human RNA polymerase II (RNAPII) hRPB11a subunit. we performed an interaction analysis using the two hybrid-system in yeast. The results confirmed that hRPB11a was indeed capable of interacting with itself. We were able to crystallise the purified hRPB11a subunit. The X-ray diffraction patterns at 3,4 Å resolution allows to infer the structure of an homodimer of the hRPB11a protein
Etude du rôle fonctionnel des IgG dans la susceptibilité au paludisme chez de jeunes enfants béninois by Rafiou Adamou( )

1 edition published in 2016 in French and held by 2 WorldCat member libraries worldwide

The aim of this thesis was to study the role of antibodies in susceptibility or resistance to malaria in young Beninese children naturally exposed to malaria infections during their first two years of life. Two complementary projects (PALNOUGENENV and TOLIMMUNPAL) were implemented in Benin to identify individual factors of malaria susceptibility. The PALNOUGENENV cohort included 600 mothers at delivery and their children from birth to 18 months of age in order to study the effects of placental malaria infection in mothers on first occurrence of malaria infections in newborns. In the TOLIMMUNPAL cohort 400 mothers were included at the first antenatal visit and followed until delivery while their infants were followed from birth to 24 months in order to study environmental determinants, biological and genetic involved in the development of immune tolerance associated with malaria and its impact on the protection of pregnant women and infants. Specifically, the association between malaria infection and the level of antibodies specifically directed against the most advanced vaccine candidate antigens of the erythrocyte stage on one hand and the ability of antibodies to inhibit in vitro the development of P. falciparum on the other hand were investigated. Our results show an association in the cohort PALNOUGENENV between high levels of IgG1 to MSP1 19 vaccine candidate antigens (p <0.001, OR = 0.90) and IgG3 to MSP2 3D7 (p <0.001, OR = 0.89) and protection against malaria infection. In the TOLIMMUNPAL cohort, high levels of IgG2 to GLURP R2 (p = 0.05, OR = 2.10) were instead associated with an increased risk of malaria infection. Functional analysis of IgG using the GIA test revealed that the infection with P. falciparum at the sampling time affected the ability of IgG to inhibit parasite growth in vitro. The purified IgG from individuals infected with P. falciparum when the sample was collected had an average 19% lower capacity of inhibition of parasite growth (p = 0.003, Wilcoxon matched pairs test) than those that were uninfected at the time of sampling. An inverse correlation was found between age and the ability of antibodies to inhibit in vitro invasion of red blood cells by the parasite. There was no association between antibody levels and ability to inhibit the in vitro parasite development. In the development of the Antibody-Dependent Respiratory Burst (ADRB) assay, the promyelocytic cell line PLB 985W was used. These cells have the capacity to differentiate into neutrophils after exposure to DMSO. Our results showed a low ability of this cell line to produce reactive oxygen species (ROS) compared to human neutrophils and ROS levels produced by this cell line are insufficient to be used in the test ADRB. Our results confirm the important role of MSP1 19 and MSP2 3D7 vaccine candidate antigens in malaria protection. Although the levels of antibodies to MSP1 19 and MSP2 3D7 were associated with decrease risk of P. falciparum infection, the functional study of antibody using the GIA assay did not allow demonstrating the relationship to protection. Investigation on the functional role of antibodies is complex as IgG could act through multiple direct or indirect mechanisms. We will continue to investigate the functional role of antibody, particularly in plasma samples from our two birth cohorts by using the ADRB assay. Results will aid in providing new information to the existing knowledge gap and will help in malaria vaccine development
Les prolamines du caryopse du Mil Pennisétum typhoideum : localisation, caractérisation et variabilité génétique by Ambaliou Sanni( Book )

1 edition published in 1981 in French and held by 2 WorldCat member libraries worldwide

Prevention by vitamin E of DNA fragmentation and apoptosis induced by fumonisin B1 in C6 glioma cells by Théophile A Mobio( )

1 edition published in 2000 in English and held by 2 WorldCat member libraries worldwide

Antimicrobial, antioxidant, toxicity and phytochemical assessment of extracts from Acmella uliginosa, a leafy-vegetable consumed in Bénin, West Africa by Latifou Lagnika( )

1 edition published in 2016 in English and held by 2 WorldCat member libraries worldwide

The ears of the African elephant: unexpected high seroprevalence of Plasmodium ovale and Plasmodium malariae in healthy populations in Western Africa by Cécile Doderer-Lang( )

1 edition published in 2014 in English and held by 2 WorldCat member libraries worldwide

Identification of novel major and minor QTLs associated with Xanthomonas oryzae pv. oryzae (African strains) resistance in rice (Oryza sativa L.) by Gustave Djedatin( )

1 edition published in 2016 in English and held by 2 WorldCat member libraries worldwide

Caractérisation structurale et biologique de nouveaux agents antibactériens naturels actifs dans les infections intestinales : des peptides de la chromogranine A et des principes actifs de Chromolaena odorata by Ménonvè Atindehou( )

1 edition published in 2012 in French and held by 1 WorldCat member library worldwide

The first bacterial strains resistant to antibiotics appeared 70 years ago and have proliferated in recent years causing a serious public health problem. Such bacteria are responsible of several types of infections including intestinal infections with chronic inflammatory bowel diseases and cancers. This work consisted of proposing new therapeutic tools in the treatment of intestinal pathologies. In this context, we have studied antimicrobial peptides derived from chromogranin A and plant extracts used in Beninese traditional medicine for the treatment of such diseases. Chromogranin A is a protein produced by nervous, endocrine and immune cells during a stress and processed to generate biologically active peptides. We identified antimicrobial peptides, active against four pathogenic bacterial strains (Klebsiella oxytoca, Salmonella enterica, Shigella sonnei and Vibrio cholera non O1) and analyzed the bacteria-peptide interactions. Moreover, the study of the peptide-antibiotic combination shows that cateslytin is useful for reducing doses of antibiotic drugs. In addition of this work, we have studied the effects of two peptides derived from chromogranin A on neuroendocrine cells with model of BON cells. Chromofungin stimules BON cells by inducing an influx of extracellular calcium, whereas catestatin is able to block chromofungin's activity.With plant extracts, after a screening on 14 plants from Benin, our works enabled us to isolate two active molecules, sinensetin and O-tetramethylether scutellarein, responsible of the antimicrobial activity of Chromolaena odorata against the studied pathogenic strains
Contribution à l'étude de l'ARN polymérase II de Plasmodium falciparum by Adonis Hazoumé( )

1 edition published in 2009 in French and held by 1 WorldCat member library worldwide

The parasite Plasmodium falciparum is the causative agent of the most burdensome form of human malaria. His RNA polymerase II (RNAPII) is responsible for transcription of protein coding genes. The sequencing of the full genome of the parasite enabled us to recover a complete set of genes sequences encoding the putative RNAPII subunits of the parasite. We have cloned those subunits by genetic reconstitution. We investigated the functional conservation of the Plasmodium RNAPII subunits using a genetic test in the yeast Saccharomyces cerevisiae. The subunits PfRPB4-5-7-9 and 12 can complement defective yeast mutants. Those results demonstrate that those subunits are orthologs of yeast conterparts. We establish then some stable yeast strains expressing Plasmodium proteins. No interspecific complementation was observed for the subunits PfRPB3-6-8-10-11. We construct viable yeast strain where the residus implicated in the interaction of the mushrom toxin alpha-amanitin where substituted by their homologs in Plasmodium and human. We screen those strains with a chemical compounds library. We don't find any drug enable to distinguish the modified strains from wild-type one. But this screen, consisting in a simple cellular test and easy to perform, could permit to identify drugs that inhibit the transcriptional activity of the parasite enzyme. Those chemical compounds will the fist step towards the discovery of a potential new class of antimalarials drugs. In this work, we also study the human RNA polymerase II (RNAPII) hRPB11a subunit. we performed an interaction analysis using the two hybrid-system in yeast. The results confirmed that hRPB11a was indeed capable of interacting with itself. We were able to crystallise the purified hRPB11a subunit. The X-ray diffraction patterns at 3,4 Å resolution allows to infer the structure of an homodimer of the hRPB11a protein
Étude de pollution atmosphérique en Afrique Sub-Saharienne : Cas de Cotonou (Bénin) : Caractérisation physicochimique des matières particulaires d'origine urbaine et impact toxicologique sur des cellules épithéliales bronchiques humaines (BEAS-2B) cultivées in vitro by Fresnel Boris A Cachon( )

1 edition published in 2013 in French and held by 1 WorldCat member library worldwide

La pollution de l'environnement, et particulièrement la pollution atmosphérique et son possible impact sur la santé humaine, suscite un intérêt grandissant dans les pays en voie de développement. Ce travail de thèse s'inscrit dans cette thématique et constitue une étude pionnière en Afrique, et au Bénin plus particulièrement. Nos résultats ont montré que l'essence utilisée à Cotonou était de piètre qualité, caractérisée par l'absence d'additifs et par une forte concentration en benzène, et pouvait être à l'origine de la dégradation de la qualité de l'air de la ville. De même, l'analyse d'échantillons de particules (PM₂.₅ et PM>₂.₅) prélevés dans le quartier de St Michel à Cotonou, a mis en exergue un taux anormalement élevé de particules en suspension dans l'air. La caractérisation physicochimique de ces particules a montré la présence d'un cocktail de composés chimiques (ions, métaux, COVs, paraffines, HAPs, etc.), en proportion plus élevées dans les particules fines PM₂.₅. L'appareil respiratoire constituant la principale voie d'exposition de l'Homme à ces particules atmosphériques, leur potentiel toxique a été évalué au travers d'une étude in vitro menée sur des cellules épithéliales bronchiques humaines (BEAS-2B). Il a été ainsi démontré les propriétés cytotoxiques des deux échantillons de particules ainsi que leur capacité à induire l'expression génique des enzymes de métabolisation des composés organiques et à générer un stress oxydatif. Le déclenchement du processus inflammatoire via l'induction de l'expression génique et la sécrétion protéique de cytokines et l'apparition de modifications dans la voie de l'acide arachidonique (leucotriènes, prostaglandine, thromboxane) ont été notés. Enfin l'approche comparative des PM₂.₅ prélevées au niveau de trois villes d'Afrique de l'Ouest a permis de mettre en évidence des caractéristiques proches au Bénin, en Côte d'Ivoire et au Sénégal et la contribution des émissions du trafic routier dans la pollution de l'air
 
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English (13)

French (9)