WorldCat Identities

Gué, Anne-Marie (19..-....).

Overview
Works: 39 works in 60 publications in 2 languages and 72 library holdings
Roles: Thesis advisor, Author, Opponent, Other
Publication Timeline
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Most widely held works by Anne-Marie Gué
Contribution à l'étude des défauts créés par irradiation électronique dans le tellurure de cadmium by Anne-Marie Gué( Book )

4 editions published in 1985 in French and held by 10 WorldCat member libraries worldwide

Containing papers presented at the European Materials Research Society 1995 Spring Meeting, Symposium G : Atomic scale characterization and simulation of materials and processes, May 22-26, 1995, Strasbourg, France by Symposium Atomic Scale Characterization and Simulation of Materials and Processes( Book )

3 editions published in 1996 in English and held by 8 WorldCat member libraries worldwide

Integration of thrombin-binding aptamers in point-of-care devices for continuous monitoring of thrombin in plasma by Ana Trapaidze( Book )

3 editions published in 2015 in English and held by 3 WorldCat member libraries worldwide

Thrombin is the central enzyme in the process of hemostasis. Normally, in vivo concentration of thrombin is rigorously regulated; however, clinically impaired or unregulated thrombin generation predisposes patients either to hemorrhagic or thromboembolic complications. Monitoring thrombin in real-time is therefore needed to enable rapid and accurate determination of drug administration strategy for patients under vital threat. Aptamers, short single-stranded oligonucleotide ligands represent promising candidates as biorecognition elements for new-generation biosensors. The aim of this PhD work therefore is to investigate different solutions for the integration of thrombin-binding aptamers in point-of-care devices for continuous monitoring of thrombin in plasma. The kinetics of aptamer interaction with thrombin and specificity towards prothrombin and thrombin - inhibitor complexes was rigorously investigated using Surface Plasmon Resonance. These experiments unveiled the complex character of interaction of the HD1 with thrombin, confirming nonspecific interactions with prothrombin, natural inhibitors of thrombin, serum albumin whereas another 29-bp aptamer HD22 proved to be highly affine and specific towards thrombin. On the other hand we explored aptamer integration options. We validated the principle and at the same managed to detect different concentrations of thrombin (5-500 nM). We finally proposed a novel approach to increase sensitivity and specificity for thrombin detection based on the engineering of aptadimer structures bearing aptamers HD1and HD22 interconnected with a nucleic acid spacer
Développement d'outils microfluidiques appliqués à la biologie : réalisation de dispositifs de tri cellulaire magnétique vertical by Sébastien Cargou( Book )

3 editions published between 2014 and 2015 in French and held by 3 WorldCat member libraries worldwide

Grâce aux avancées de la recherche de ces dix dernières années, qui ont permis l'implémentation de plus en plus de fonctions au sein d'un système microfluidique, les " laboratoires sur puce " connaissent un essor important notamment dans le domaine de la santé. Les premiers dispositifs voient le jour dans le domaine commercial, mais un verrou important reste d'identifier des éléments circulants en faible concentration dans le sang par exemple. Les techniques de séparation simples, efficaces et modulables sont la clef de cette problématique. Nous présentons dans ce travail une voie originale de séparation magnétique grâce à des bobines planaires en cuivre combinées à un système microfluidique 3D permettant une séparation verticale efficace. L'étude consiste en la conception, la modélisation, la réalisation et le test de dispositifs appliqués à des solutions tests constituées de billes magnétiques dans un premier temps, puis de composés biologiques de culture. La réalisation des laboratoires sur puce a été faite par des procédés de photolithographie et grâce à une technologie originale de laminage de film sec. De par sa très bonne robustesse, dans le temps et face aux solvants, ainsi que sa biocompatibilité, la SU-8 est un candidat de choix pour la réalisation de ces LOC. Cette technologie nous a permis de réaliser des dispositifs tout-polymère tridimensionnels et intégrant des fonctions actives dont l'actionnement magnétique. La microélectronique a depuis longtemps permis de miniaturiser tous les éléments électroniques courants tels les capteurs et les bobines. Il est alors très intéressant de voir comment nous pouvons les intégrer dans un dispositif microfluidique pour créer des fonctions plus complexes. Nous avons pu ainsi démontrer une séparation continue de monocyte THP1 de culture avec une efficacité de 82% et des voies d'amélioration prometteuses validées avec des billes magnétiques
Conception et réalisation d'un micro-injecteur matriciel pour la fonctionalisation des biopuces by Ty Phou( Book )

2 editions published in 2003 in French and held by 2 WorldCat member libraries worldwide

Conception et réalisation d'un « lab on chip » pour la mesure de polluants organiques dans les eaux de rejet by Amani Migaou( Book )

2 editions published in 2019 in French and held by 2 WorldCat member libraries worldwide

The main objective of the thesis is to study and develop a biosensor dedicated to the water quality control. Its operating mode will be based on the use of some specialized bacteria chosen for their particular sensitivity to organic molecules. They will be immobilized in instrumented wells designed to optimize their electro-optical responses to stimuli caused by chemical and biological reactions. A monitoring will be set up to control the parameters of exchanges between these bacteria and the wastewater sample in which they are immersed. The material used to develop these bioMEMS will be chosen in order to ensure biocompatibility and also the miniaturization process of device fabrication. Afterwards, their insertion in an instrumental setup will be studied to achieve the optimal conditions to measure the BOD parameters of the water samples. In addition to this part, a modeling approach of bacteria-oxygen interactions will be undertaken to compare the theoretical and experimental results so that the BOD value will be obtained in the shortest time possible
Conception et réalisation d'une matrice de microéjecteur thermique adressable individuellement pour la fonctionnalisation de biopuce by David Jugieu( Book )

2 editions published in 2005 in French and held by 2 WorldCat member libraries worldwide

Cette thèse porte sur la conception et la réalisation d'un microsystème d'éjection matriciel monolithique pour la fonctionnalisation in-situ des puces à ADN. Le principe s'inspire du jet d'encre thermique mais l'originalité vient du fait que les éjecteurs sont disposés de façons matricielle avec une très grande densité et sont actionnables individuellement. La structure retenue intègre une résistance chauffante sur une membrane diélectrique et une buse d'éjection réalisée en résine photosensible Su8. Ces caractéristiques permettent d'atteindre localement des températures suffisamment élevées pour provoquer une ébullition localisée autour de la buse déjection. Un procédé technologique original a été mis au point pour intégrer le dispositif d'adressage à cette résistance. Les matrices réalisées ont été caractériésées électriquement et thermiquement ; il a été ainsi montré que l'adressage est opérationnel, la puissance thermique offerte est suffisante pour l'éjection
Microfluidique 3D et actionneurs magnétiques : de leur intégration à la préparation d'échantillons biologiques by Marc Fouet( Book )

2 editions published in 2016 in French and held by 2 WorldCat member libraries worldwide

Microfluidic chips are key elements for solutions and biological samples handling and analysis. They are enablers for micro-scale studies and are the cornerstone of lab on chips, at the cutting edge of medical diagnostics. The aim of this thesis work was to explore functional possibilities offered by 3D microfluidic architectures for the development of diagnostic tools relying on cell sorting, tagging and handling. These functions were investigated on monocytes sub-populations, which are markers for many inflammatory diseases. In order to cover a consistent series of necessary steps for complex biological samples pretreatment, three additional functions were studied: size sorting with hydrodynamic filtration, immuno-isolation by magnetic separation, and on-chip tagging with magnetic microparticles. To perform tagging reactions, a micromixer based on diffusion and flow split and recombination (baker's transform) was fabricated and characterized. Analytical (diffusion) and numerical (diffusion-advection) models are showed, together with test experiments on the devices for mixing reactions of fluorescein/water and cells/microbeads. New approaches of hydrodynamic filtration based size sorting were investigated by devising 3D bypass structures, that allow developing a mixing strategy (tagging reactions) suited to cells and particles. An analytical model for flows and sorting efficiency is introduced and compared to the devices characterization. Furthermore, it was shown that this approach also enables sorting of sub-micron particles (like blood microparticles). All 3D microfluidic systems were obtained thanks to an original dry film photoresist stacking (lamination) technique, dramatically reducing micro-fabrication time, even though compatible with standard process. This fabrication technique also enables magnetic micro-sources integration in lab on chips by realizing planar micro-coils underneath microfluidic channels. By coupling the effects of integrated micro-coils to the fields generated by external magnets, we brought the proof of concept of systems dedicated to trapping, focusing and separating (in flow) magnetic microbeads. Models (magnetic fields and forces) are described along with devices characterization. Conception of specific instrumentation (current source) for micro-coils actuation is also shown, as it allows time and intensity control over applied magnetic fields
Etude et développement d'une plateforme microfluidique dédiée à des applications biologiques : intégration d'un actionneur magnétique sur substrat souple by Rémy Fulcrand( Book )

2 editions published in 2009 in French and held by 2 WorldCat member libraries worldwide

Les mutations profondes observées au cours des vingt-cinq dernières années dans le monde de l'électronique ont actuellement cours en biologie, en partie grâce aux biotechnologies. Le concept communément appelé "laboratoire sur puce" (Lab-On-Chips, LOC) doit son essor à l'effort considérable qui a été mené en matière de développement des technologies de micro-fabrication. Le nombre d'outils technologiques disponibles actuellement pour la fabrication de réseaux microfluidiques est important mais la réalisation de systèmes complexes tridimensionnels reste encore un challenge. Le développement des micro- et nanotechnologies à de nouveaux matériaux comme les polymères, et en particulier la SU-8, a permis d'ouvrir la voie à une intégration plus élaborée telle que nous la présentons dans ce travail de thèse. Nous y exposons la démarche et les détails d'une approche consistant en la conception, la modélisation et la fabrication de dispositifs d'actionnement magnétique intégrés au sein de réseaux microfluidique en SU-8 pour la manipulation de billes magnétiques ; le tout étant élaboré sur un substrat souple de type PET. Le développement des outils de caractérisations des systèmes réalisés a également été présenté. Ils ont permis la mise en place d'un protocole expérimental visant à évaluer et quantifier les différents paramètres électriques, thermiques, fluidiques et magnétiques concourant à obtenir un dispositif LOC efficient dans la circulation de fluides, le piégeage de billes magnétiques, leur transfert d'une zone à un autre et leur orientation guidée vers des canaux privilégiés
Conception, réalisation et caractérisation de micro-miroirs à déflexion localisée appliqués aux télécommunications optiques by Bruno Estibals( Book )

2 editions published in 2002 in French and held by 2 WorldCat member libraries worldwide

La demande d'acroissement des capacités des réseaux de transmission de données a fait naître un besoin en composants tout optique. Le principal objectif de cette thèse a été de mettre au point une nouvelle architecture de micro-miroir et d'en faire l'évaluation de ses performances. Celle-ci était destinée à être implantée dans des multiplexeurs-démultiplexeurs en longueurs d'ondes ou dans des routeurs optiques. Deux approches comparatives ont été menées, une analytique et une par simulation. Elles ont permis la réalisation puis la caractérisation de micro-miroirs qui sont aujourd'hui commercialisables. Pour des raisons de miniaturisation, nous nous sommes intéressé aux alimentations intégrées spécifiques nécessaires à ce type d'application. Après un état de l'art des solutions existantes pour en déduire des solutions compatibles en taille avec notre application, nous présentons notre contribution à l'avancement des travaux permettant l'obtention d'éléments passifs de stockage
Laboratoire sur puce pour la détection d'événements cellulaires rares by Marion Valette( Book )

2 editions published in 2019 in French and held by 2 WorldCat member libraries worldwide

Le tissu adipeux est une source riche en cellules souches multipotentes : les Cellules Souches Adipeuses (ASCs pour Adipose Stem Cells). Ces cellules, qui possèdent la capacité de se différencier en différents types cellulaires, ouvrent de nombreuses perspectives dans le domaine de la médecine régénératrice et dans des applications telles que le diagnostic du diabète de type 2. Connues pour migrer et circuler dans la lymphe, l'hypothèse de leur présence dans le sang n'est pas exclue mais aucune méthode n'existe afin de le prouver. L'objectif de ces travaux de thèse est alors de développer un laboratoire sur puce capable d'isoler les ASCs à partir d'échantillons biologiques complexes en mettant en application des méthodes microfluidiques de tri passives et sans marquage. Ce sont ainsi les propriétés intrinsèques des cellules qui sont exploitées. Or, les ASCs ne présentent aucune caractéristique physique spécifique. En effet, nous avons tout d'abord montré que leur diamètre est compris entre 10 et 25 µm, ce qui ne leur permet pas de se distinguer de la plupart des cellules sanguines. De même, ces cellules ne possèdent pas d'antigène spécifique sur leur membrane. Nous proposons alors un dispositif combinant deux étapes complémentaires afin d'isoler complètement les ASCs des autres types cellulaires. La première étape a pour objectif de prétraiter l'échantillon en retirant, par filtration hydrodynamique, toutes les cellules de diamètre inférieur à 10 µm. Ce dispositif doit ainsi permettre de retirer du milieu les globules rouges qui représentent plus de 99 % des cellules constituant le sang ainsi que les plaquettes et quelques globules blancs. Ces travaux de thèse ont démontré que le dispositif développé est capable de prétraiter efficacement un échantillon sanguin pur (humain ou murin) en éliminant plus de 99,9 % des globules rouges. De plus, il a été démontré que la filtration n'engendre pas de lyse cellulaire, ce qui est encourageant pour des questions de viabilité cellulaire et l'exploitation des cellules après filtration. L'échantillon alors obtenu contient les cellules d'intérêt ainsi que quelques cellules hématopoïétiques restantes. La deuxième étape a pour but de parfaire l'isolement des ASCs en les séparant des cellules hématopoïétiques restantes. Pour ce faire, la méthode employée, l'exclusion immunologique par cell rolling, se base sur la spécificité de la réaction antigène-anticorps. Les ASCs ne possédant pas d'antigène spécifique, ce sont les antigènes spécifiques des leucocytes qui ont été ciblés. L'objectif est ainsi de dépléter l'échantillon des leucocytes restants. Ces travaux ont mené à l'élaboration d'un protocole de fonctionnalisation de surface optimal. De plus, de premiers résultats encourageants sur le cell rolling sur une surface fonctionnalisée avec des anticorps anti-CD45 ont été obtenus
Microbubbles for optofluidics: controlled defects in bubble crystals by Alaa Allouch( )

1 edition published in 2014 in English and held by 2 WorldCat member libraries worldwide

A multilevel polymer process for direct encapsulation of fluids in microfluidic systems by Remy Bossuyt( )

1 edition published in 2011 in English and held by 2 WorldCat member libraries worldwide

Microtechnologies polymères pour les laboratoires sur puces by Patrick Abgrall( Book )

2 editions published in 2006 in French and held by 2 WorldCat member libraries worldwide

The invention of µTAS concept (micro total chemical analysis system) in the early nineties introduced fluids in the microsystems world. A lab on chip integrates all the functions of a macroscopic laboratory (handling, mixing, heating liquids, filtering, separating, detecting molecules etc.) on a small surface (typically a few square centimetres). The technological challenge lies on the coupling between a conventional microsystem and a microfluidic network. As silicon and glass processes have been widely used in the nineties, they demonstrated major drawbacks: incompatibility of silicon technologies with high electric fields required for electrophoretic separations and/or electroosmotic pumping, inadequate technologies for large surfaces processing, integration issues in a complete system, high cost of the materials and associated processes, etc. The proposed solution in this thesis consist on directly constructing the microfluidic network above the conventional microsystem in photosensitive resists (SU-8), making easier the integration and allowing 3D structures fabrication with an excellent level to level alignment. Microfluidic characterization tools developed and used during this work are presented. Surface effects becoming essential at this scale, a generic modification strategy of physicochemical properties of SU-8 is also proposed and characterized
Matériaux mésomorphoses à empreinte moléculaire pour le développement d'un capteur de pesticides by Elisabeth Laurent( Book )

2 editions published in 2008 in French and held by 2 WorldCat member libraries worldwide

Within the framework of organophosphorus pesticides detection, the principle developed here is the use of molecularly imprinted (MIP) mesomorphous materials for a chemical sensing application. These materials have the particularity to specifically recognize the molecule for which they are produced, the template. Moreover, their mesomorphous properties allow to increase mass capacity and to enhance memory effect. In order to develop a MIP for pesticides molecular recognition, the first step consists in creating non covalent interactions between the template and functional groups. By studying this step, hydrogen bonds type interactions have been found and characterized between the chosen template, a paraoxon analogue, diethyl(4-nitrobenzylphosphonate)(DE4NBP) and two functional groups an acid and an alcohol, by 1H-NMR, IR-spectroscopy and microcalorimetry (ITC). With the aim of developing a sensing device, the material synthesis consists in two steps. Firstly, functional groups have been grafted onto a polysiloxane backbone together with liquid crystal groups and a photo-crosslinking agent by hydrosilylation. Catalysts and adequate conditions choice allows to obtain linear and grafted polymers with mesomorphous properties. Secondly, polymers have been spread to form films and then irradiated. Photo-crosslinking function activation leads to mesomorphous crosslinked materials formation. Imprinted materials recognition properties towards DE4NBP have been assessed by HPLC titration of batch rebinding solutions. The results obtained showed a goodspecificity for the alcohol-type imprinted materials compared to non-imprinted ones, at a given DE4NBP concentration. Electronic devices have been developed. Two sensing modes have been considered: a capacitive one, based on material dielectric constant variation due to concentration of the template on the MIP, and another one based on thermal principles. In this case, MIP thermal properties vary with the adsorption of template into the material. Capacitive structures design and conception have been carried out and sensing principle of both sensors has been verified
Microfluidique diphasique : réseaux de micro-bulles à défauts contrôlés pour la photonique by Alaa el dine Allouch( Book )

2 editions published in 2011 in French and held by 2 WorldCat member libraries worldwide

Microfluidics is a large field that encompasses the study of fluids' behavior at microscales. Progresses in microfabrication technologies have enabled an increasing number of applications in biology, chemistry, and recently in optics. Indeed, microfluidic methods to fabricate photonic crystals are attractive, as compared to standard microfabrication, since they enable low interfaces roughness and self-organized collective fabrication. In this perspective, this thesis suggests the use of two-phase microfluidics to fabricate stable bubble networks, and to integrate easily realistic optical functionalities. First of all, we present the formation of monodisperse hexagonal bubble networks with a tunable pitch (between 5 and 100 µm) controlled by geometry and flow conditions. High-quality crystal organization is revealed by diffraction imaging. A photopolymer, used as carrier liquid, enables obtaining long life crystals. We have developed a glass-glass technology which allows the fabrication of channels adapted to optical applications: transparent, rigid and chemically resistant. To further demonstrate the potentiality of our approach, we have realized bubble crystals which contain controlled defects (lacuna of one, two or line of bubbles), key element in the design of waveguides or resonators. Small bumps are used in order to exclude bubbles from determined zones by a competition between interfacial tension and hydrodynamic forces. We have developed and experimentally confirmed a model which predicts the efficiency of this method. On-chip generated microbubbles are promising for photonics: they intrinsically enable self-organization and surface roughness unbeatably low. Sub-wavelength period are still needed to build photonic crystal-based optical functions. Our approach should enable such scaling down, because bubble formation is not diffraction limited as opposed to photolithography. This work thus opens a novel way to achieve optical functionalities such as waveguide, filter or resonator
Dispositif microfluidique pour la quantification de sous-populations de cellules by Rémi Manczak( )

2 editions published in 2016 in French and held by 2 WorldCat member libraries worldwide

The quantitative detection of specific cells is usually carried out by flow cytometry due to its high sensitivity and reliability, however, this technique is not suited for routine screening and point-of-care diagnostics. Electrochemical methods, as electrochemical impedance spectroscopy have gained interest mainly due to a label-free detection and their miniaturization capability required for integration on chip. Furthermore, recent advances in microfabrication based technologies have allowed to develop micron-sized electrodes whose main advantages over conventional electrodes are higher impedances due to smaller currents and the possibility of being integrated inside microfluidic channels. The aim of the present work was the realization and the optimization of microfluidic devices with improved sensitivity targeting the immuno-trapping and counting of pro-inflammatory monocytes as infection markers. Taking into account the influence of the surface coverage on the sensitivity, different geometries were tested. The best sensitivities and reproducibility were recorded in the case of interdigitated micro-electrodes with weak inter-electrodes gap (50 µm). Moreover, experiments carried out with different surfaces demonstrated that there was a threshold beyond which a surface is exploitable for a given slice of concentration. Such microfluidic devices allowed to reach a detection limit around 10 cells/mL. Furthermore, due to the high sensitivity recorded, the devices were also tested to detect ligand binding by cell receptors. These studies have allowed to demonstrate the interaction of CHO-A2a with c-di-AMP for low cell concentrations
Biocapteur pour la surveillance de la qualité de l'eau : Application aux eaux pluviales et de stations d'épurations by Loïc Recoules( Book )

2 editions published in 2015 in French and held by 2 WorldCat member libraries worldwide

In recent years, new regulations concerning environmental protection have emerged, particularly with the Water Framework Directive proposed in 2000. It imposes standards more restrictive on discharges from wastewater systems in receiving environments. These discharges are even more consistent than the rainfall events are more intense, causes of climate change, and population increases as outlined in the Plan National Santé Environnement (2009-2013). The test of the Biological Oxygen Demand (BOD) is to date the most widely used to meet these standards. However, its achievement time does not allow managers sewerage systems to anticipate pollution peaks, and to retroact on the purification process prior to discharge into receiving environment. In addition, this standardized test uses a bacterial inoculum from the environment, containing different strains with the diversity and concentration is unknown. This does not, then, to have a good reproducibility of the measurement of the BOD. It is in this environmental protection context that the BIOGUARD project, result of an agreement between academic and industrial research in the field, is positioned. As part of these thesis works, we developed tools and methods to reduce both the volume of sample used for the measurement with a micro-integration approach, and both the duration of the analysis by an optimization of the biodegradation process by bacteria, and by mathematical modeling approach. Two types of optical sensors have been used, the first for measuring dissolved oxygen, and the second to measure the bacterial activity. Both sensors have been integrated into microfluidic devices in PDMS-glass technology, a technology perfectly suited to this type of research because of its biocompatibility, its simplicity of implementation and its great adaptability. One of the innovations of this project is to provide a biosensor combining responses to several bacterial strains specifically selected to increase the accuracy and reproducibility of the measurement. In our experiments, however, a single bacterial strain is used. A study of influencing factors was conducted to observe changes in bacterial behavior. With the experimental results, we have tried to explain these changes by a mathematical approach, using theoretical models of bacterial growth, in order to predict the value of BOD. Finally, a macro-fluidic prototype, based on a single bacterial strain, reusable and automatic was also developed to provide a simple fluidic architecture that could be duplicated for use with all strains included in the project
Capteurs chimiques au silicium à modulation du champ électrique de surface by Amal Akheyar( Book )

1 edition published in 1997 in French and held by 2 WorldCat member libraries worldwide

CE TRAVAIL CONTRIBUE A L'ETUDE DES FACTEURS LIMITANT LA SELECTIVITE ET LA SENSIBILITE DES CAPTEURS CHIMIQUES DE TYPE CHEMFET OU GASFET. CES LIMITATIONS RESULTENT, DE NOTRE POINT DE VUE, DE <<<>L'EMPOISONNEMENT<>>> DES SITES DE FIXATION DES ESPECES SUR LES SURFACES ACTIVES. CETTE ETUDE PROPOSE UNE MODELISATION DU FONCTIONNEMENT DES GASFET BASEE SUR L'ADSORPTION ET LA DESORPTION. CELLE-CI TRAITE EN PARTICULIER DE LA SELECTIVITE LORSQUE DEUX ESPECES SONT EN PRESENCE. ELLE MONTRE, PAR SIMULATION, TOUT L'INTERET QU'IL Y A A APPLIQUER DES METHODES DE MESURE TRANSITOIRES. LES TRANSITOIRES THERMIQUES SONT ETUDIEES ET NOUS METTONS L'ACCENT SUR LES VARIATIONS BRUSQUES DU CHAMP ELECTRIQUE DE SURFACE. SUR LA BASE DE NOS CONCLUSIONS THEORIQUES, NOUS PROPOSONS UNE STRUCTURE ORIGINALE, FONCTIONNANT EN MILIEU LIQUIDE ET GAZEUX. POUR LA REALISATION DE CETTE STRUCTURE UN PROCEDE TECHNOLOGIQUE COMPLET A ETE MIS AU POINT, SIMULE PAR LE LOGICIEL SUPPREM IV. CE TRAVAIL SE TERMINE PAR LA CARACTERISATION ELECTRIQUE DE CETTE STRUCTURE. LES RESULTATS OBTENUS MONTRENT LA FAISABILITE DES CONCEPTS ET DES PRINCIPES TECHNOLOGIQUES QUI ONT ETE PROPOSES
Technologies PNIPAM pour les laboratoires sur puce by Guillaume Paumier( )

1 edition published in 2009 in French and held by 1 WorldCat member library worldwide

Labs on chips are miniaturized devices integrating one or several laboratory functions, usually dedicated to the handling of chemical and biological samples. Our work aimed at integrating a smart polymer called poly(N-isopropylacrylamide) (PNIPAM) in microsystems, in order to develop a new technological process for labs on chips. PNIPAM is a thermosensitive polymer that undergoes a reversible state transition; it switches from a hydrophilic, swollen state below its temperature of transition (LCST ~ 32°C), to a hydrophobic, collapsed state above it. The technology we developed is based on heating elements and a surface functionalization process to graft the PNIPAM layer. Our results show that the electro-osmotic flow can be modulated by thermally controlling the PNIPAM, thus paving the way to electrokinetic mixers. This thermal control also enables the adsorption (and partial desorption) of proteins on fonctionalized beads, the main application being sample preparation
 
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Alternative Names
Anne-Marie Gué Frans academisch docent

Gué, Anne-Marie

Languages
French (33)

English (8)