WorldCat Identities

Sowa, Sławomir

Overview
Works: 30 works in 33 publications in 2 languages and 36 library holdings
Roles: Author, Other
Publication Timeline
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Most widely held works by Sławomir Sowa
Overview and recommendations for the application of digital PCR European Network of GMO Laboratories (ENGL)( )

2 editions published in 2019 in English and held by 3 WorldCat member libraries worldwide

The so-called digital Polymerase Chain Reaction (dPCR) is a relatively new technique for the detection and quantification of DNA, but its application in analytical laboratories is steadily increasing. In contrast to quantitative real-time PCR, DNA (fragments) can be quantified here without the need for calibration curves. Using dPCR, the PCR mix containing the (target) DNA is partitioned - depending on the device used - currently into a maximum of 10,000,000 small compartments with a volume as low as a few picolitres. These can be either physically distinct compartments on a chip (referred to as chamber-based digital PCR [cdPCR]), or the compartments correspond to water-in-oil droplets (referred to as droplet digital [ddPCR]). Once the PCR has been carried out simultaneously in all compartments/droplets, it is common to both approaches that the number of positive and negative signals for each partition is counted by a fluorescence measurement. With this technique, an absolute quantification of DNA copy numbers can be performed with high precision and trueness, even for very low DNA copy numbers. Furthermore, dPCR is considered less susceptible than qPCR to PCR inhibitory substances that could be co-extracted during DNA extraction from different samples. Digital PCR has already been applied in various fields, for example for the detection and quantification of GMOs, species (animals, plants), human disease bioindicators, food viruses and bacteria including pathogens. When establishing dPCR in a laboratory, different aspects have to be considered. These include, but are not limited to, the adjustment of the type of the PCR master mix used, optimised primer and probe concentrations and the signal separation of positive and negative compartments. This document addresses these and other aspects and provides recommendations for the transfer of existing real-time PCR methods into a dPCR format
Caracal, czyli jak się to robi po francusku by Sławomir Sowa( )

2 editions published in 2015 in Polish and held by 2 WorldCat member libraries worldwide

Optimization and Verification of Droplet Digital PCR Even-Specific Methods for the Quantification of GM Maize DAS1507 and NK603 by Katarzyna Grelewska-Nowotko( )

1 edition published in 2017 in English and held by 2 WorldCat member libraries worldwide

Biogospodarka, biotechnologia i nowe techniki inżynierii genetycznej( )

1 edition published in 2017 in Polish and held by 1 WorldCat member library worldwide

A simple and efficient method for cryopreservation of embryogenic triticale calli by Sławomir Sowa( )

1 edition published in 2005 in English and held by 1 WorldCat member library worldwide

Materiały z 1st Conference of The Polish Society of Experimental Plant Biology, Olsztyn
Konieczność monitorowania genetycznie zmodyfikowanego rzepaku w Polsce by Iwona Wiśniewska( )

1 edition published in 2006 in Polish and held by 1 WorldCat member library worldwide

Ale urwał! : polskie kino dziś publiczność ma na YouTube by Sławomir Sowa( )

1 edition published in 2010 in Polish and held by 1 WorldCat member library worldwide

Zostało tylko jedno wolne miejsce by Sławomir Sowa( )

1 edition published in 2010 in Polish and held by 1 WorldCat member library worldwide

Dot. żołnierzy poległych w Iraku i w Afganistanie
Nonsymbiotic haemoglobins in plants( )

1 edition published in 1999 in English and held by 1 WorldCat member library worldwide

Jerzy Kropiwnicki - przegrany, który zawsze wie lepiej by Marcin Darda( )

1 edition published in 2010 in Polish and held by 1 WorldCat member library worldwide

Guidance document on multiplex real-time PCR methods( )

1 edition published in 2021 in English and held by 1 WorldCat member library worldwide

Polymerase Chain Reaction (PCR) is considered the gold standard technique for the detection and quantification of Genetically Modified (GM) food, feed and seeds. The combination of two or more PCR methods in a single reaction tube is referred to as multiplex PCR and is a powerful approach already adopted by many testing laboratories. Multiplex PCR can address the need for increased and more efficient testing for the presence of GMOs in food, feed or seeds and for the quantification of GM events. Although real-time PCR has been used for GMO testing for many years as well as for research, forensic or diagnostic purposes, a comprehensive guidance on the development and implementation of multiplex PCR is currently not available. This document has been developed by experts from the European Network of GMO Laboratories (ENGL). It provides background information on the technical aspects of multiplex PCR (mpPCR), including information on instruments, fluorescent dye selection and reaction controls. It gives guidance on how to implement a previously validated mpPCR method (i.e. method verification), as well as how to develop and validate a new mpPCR method. Guidance on criteria for the acceptance of methods, examples of how to assess these criteria and troubleshooting tips are given. Finally, a list of currently used mpPCR methods is included
Molekularne metody wykrywania i identyfikacji organizmów genetycznie zmodyfikowanych (GMO) by Anna Linkiewicz( )

1 edition published in 2006 in Polish and held by 1 WorldCat member library worldwide

Doublet haploids in production of male sterility maintaining triticale (Triticosecale Wittmack) lines( )

1 edition published in 2005 in English and held by 1 WorldCat member library worldwide

Materiały z 1st Conference of The Polish Society of Experimental Plant Biology, Olsztyn
Przestrzeń rekreacyjna i zasoby agroturystyczne "Krainy Żubra" by Władysław Sowa( )

1 edition published in 2009 in Polish and held by 1 WorldCat member library worldwide

Stanowisko Komitetu Biotechnologii Polskiej Akademii Nauk w sprawie nowych technik inżynierii genetycznej( )

1 edition published in 2015 in Polish and held by 1 WorldCat member library worldwide

Pollen from genetically modified plants in honey : problems with quantification and proper labelling( )

1 edition published in 2013 in English and held by 1 WorldCat member library worldwide

 
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Alternative Names
Sowa, S.

Languages
Polish (15)

English (8)