Vivien, Céline (19..-....; auteur d'une thèse en physiologie et biologie moléculaire)
Overview
| Works: | 1 works in 2 publications in 1 language and 2 library holdings |
|---|---|
| Roles: | Author |
Publication Timeline
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Most widely held works by
Céline Vivien
Régénération et reprogrammation in vivo des tissus musculaires chez Xenopus laevis by
Céline Vivien(
Book
)
2 editions published in 2014 in French and held by 2 WorldCat member libraries worldwide
In this PhD, the Xenopus laevis model was used to investigate two biological processes: in vivo reprogramming and regeneration of muscles tissues. This study addresses the question of cellular plasticity in an integrated context using two different approaches. At first it was shown that a differentiated tissue - the skeletal muscle - could be reprogrammed into a pluripotent state by the overexpression of murine reprogramming factors mOct4, mSox2 and mKlf4. Consequently, this result suggests that genetic networks underlying cellular reprogramming are evolutionary conserved between amphibians and mammals. Moreover, during the PhD the ability of cardiac regeneration during post-embryonic development in Xenopus laevis was also studied. Resections of the apex of the heart ventricle were developed and performed in Xenopus larval stages, during metamorphosis, in juveniles and adults. Following this, regeneration, or absence of such, in cardiac tissue was examined using histological and molecular approaches. The current study demonstrated a negative impact of metamorphosis on the regenerative capacity of the heart. As metamorphosis is strictly dependent on thyroid hormone signalling, the results suggest involvement of these hormones in the regeneration process. The overall results obtained during the PhD open new perspectives in understanding the control of cellular plasticity and stability of the differentiation status
2 editions published in 2014 in French and held by 2 WorldCat member libraries worldwide
In this PhD, the Xenopus laevis model was used to investigate two biological processes: in vivo reprogramming and regeneration of muscles tissues. This study addresses the question of cellular plasticity in an integrated context using two different approaches. At first it was shown that a differentiated tissue - the skeletal muscle - could be reprogrammed into a pluripotent state by the overexpression of murine reprogramming factors mOct4, mSox2 and mKlf4. Consequently, this result suggests that genetic networks underlying cellular reprogramming are evolutionary conserved between amphibians and mammals. Moreover, during the PhD the ability of cardiac regeneration during post-embryonic development in Xenopus laevis was also studied. Resections of the apex of the heart ventricle were developed and performed in Xenopus larval stages, during metamorphosis, in juveniles and adults. Following this, regeneration, or absence of such, in cardiac tissue was examined using histological and molecular approaches. The current study demonstrated a negative impact of metamorphosis on the regenerative capacity of the heart. As metamorphosis is strictly dependent on thyroid hormone signalling, the results suggest involvement of these hormones in the regeneration process. The overall results obtained during the PhD open new perspectives in understanding the control of cellular plasticity and stability of the differentiation status
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Related Identities
- Jopling, Chris Other
- Coen, Laurent Opponent
- Vriz, Sophie Other
- Muséum national d'histoire naturelle (Paris) Degree grantor
- Thièbaud, Pierre (1957-....). Other
- École doctorale Sciences de la nature et de l'Homme - Évolution et écologie (Paris) Other
- Demeneix, Barbara (1949-....). Thesis advisor
- Chanoine, Christophe Opponent
Languages