WorldCat Identities

Wojtas, Bartosz

Overview
Works: 7 works in 8 publications in 1 language and 13 library holdings
Roles: Contributor, Other
Publication Timeline
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Most widely held works by Bartosz Wojtas
Unveiling new interdependencies between significant DNA methylation sites, gene expression profiles and glioma patients survival by Michal J Dabrowski( )

2 editions published in 2018 in English and held by 3 WorldCat member libraries worldwide

In order to find clinically useful prognostic markers for glioma patients' survival, we employed Monte Carlo Feature Selection and Interdependencies Discovery (MCFS-ID) algorithm on DNA methylation (HumanMethylation450 platform) and RNA-seq datasets from The Cancer Genome Atlas (TCGA) for 88 patients observed until death. The input features were ranked according to their importance in predicting patients' longer (400+ days) or shorter (& lt;= 400 days) survival without prior classification of the patients. Interestingly, out of the 65 most important features found, 63 are methylation sites, and only two mRNAs. Moreover, 61 out of the 63 methylation sites are among those detected by the 450 k array technology, while being absent in the HumanMethylation27. The most important methylation feature (cg15072976) overlaps with the RE1 Silencing Transcription Factor (REST) binding site, and was confirmed to intersect with the REST binding motif in human U87 glioma cells. Six additional methylation sites from the top 63 overlap with REST sites. We found that the methylation status of the cg15072976 site affects transcription factor binding in U87 cells in gel shift assay. The cg15072976 methylation status discriminates & lt;= 400 and 400+ patients in an independent dataset from TCGA and shows positive association with survival time as evidenced by Kaplan-Meier plots
Histone deacetylase inhibitors exert anti-tumor effects on human adherent and stem-like glioma cells by Halina Was( )

1 edition published in 2019 in English and held by 2 WorldCat member libraries worldwide

<> by Katarzyna Szołtysek( )

1 edition published in 2018 in English and held by 2 WorldCat member libraries worldwide

Two-miRNA classifiers differentiate mutation-negative follicular thyroid carcinomas and follicular thyroid adenomas in fine needle aspirations with high specificity by Tomasz Stokowy( )

1 edition published in 2016 in English and held by 2 WorldCat member libraries worldwide

Molecular differential diagnosis of follicular thyroid carcinoma and adenoma based on gene expression profiling by using formalin-fixed paraffin-embedded tissues by Aleksandra Pfeifer( )

1 edition published in 2013 in English and held by 2 WorldCat member libraries worldwide

Somatic mutation profiling of follicular thyroid cancer by next generation sequencing( )

1 edition published in 2016 in English and held by 1 WorldCat member library worldwide

Abstract: The molecular etiology of follicular thyroid tumors is largely unknown, rendering the diagnostics of these tumors challenging. The somatic alterations present in these tumors apart from RAS gene mutations and PAX8 / PPARG translocations are not well described. To evaluate the profile of somatic alteration in follicular thyroid tumors, a total of 82 thyroid tissue samples derived from 48 patients were subjected to targeted Illumina HiSeq next generation sequencing of 372 cancer-related genes. New somatic alterations were identified in oncogenes (MDM2, FLI1), transcription factors and repressors (MITF, FLI1, ZNF331), epigenetic enzymes (KMT2A, NSD1, NCOA1, NCOA2), and protein kinases (JAK3, CHEK2, ALK). Single nucleotide and large structural variants were most and least frequently identified, respectively. A novel translocation in DERL / COX6C was detected. Many somatic alterations in non-coding gene regions with high penetrance were observed. Thus, follicular thyroid tumor somatic alterations exhibit complex patterns. Most tumors contained distinct somatic alterations, suggesting previously unreported heterogeneity. Highlights: The spectrum of somatic alterations in follicular thyroid tumors is largely unknown. Cancer genes in 82 thyroid tissue samples were tested by next generation sequencing. Single nucleotide variants were most common; large structural changes were rare. New somatic alterations were found in genes as well as non-coding regions. Distinct tumor somatic alterations suggested previously unreported heterogeneity
Mapping chromatin accessibility and active regulatory elements reveals pathological mechanisms in human gliomas by Karolina Stepniak( )

1 edition published in 2021 in English and held by 1 WorldCat member library worldwide

Chromatin structure and accessibility, and combinatorial binding of transcription factors to regulatory elements in genomic DNA control transcription. Genetic variations in genes encoding histones, epigenetics-related enzymes or modifiers affect chromatin structure/dynamics and result in alterations in gene expression contributing to cancer development or progression. Gliomas are brain tumors frequently associated with epigenetics-related gene deregulation. We perform whole-genome mapping of chromatin accessibility, histone modifications, DNA methylation patterns and transcriptome analysis simultaneously in multiple tumor samples to unravel epigenetic dysfunctions driving gliomagenesis. Based on the results of the integrative analysis of the acquired profiles, we create an atlas of active enhancers and promoters in benign and malignant gliomas. We explore these elements and intersect with Hi-C data to uncover molecular mechanisms instructing gene expression in gliomas. Gliomas are tumors often associated with epigenetics-related gene deregulation. Here the authors reveal an atlas of active enhancers and promoters in benign and malignant gliomas by performing whole-genome mapping of chromatin accessibility, histone modifications, DNA methylation patterns and transcriptome analysis simultaneously in multiple tumor samples
 
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