WorldCat Identities

Charroux, Bernard (biologiste cellulaire)

Overview
Works: 7 works in 11 publications in 2 languages and 12 library holdings
Genres: Academic theses 
Roles: Author, Other, Opponent, Thesis advisor
Publication Timeline
.
Most widely held works by Bernard Charroux
La sympathectomie thoracique haute : à propos de 64 interventions by Bernard Charroux( Book )

3 editions published in 1974 in French and held by 4 WorldCat member libraries worldwide

Contribution à l'étude fonctionnelle de la protéine hnRNP K chez Drosophila melonogaster by Bernard Charroux( Book )

3 editions published in 1997 in French and held by 3 WorldCat member libraries worldwide

LE TRAVAIL PRESENTE DANS CE MEMOIRE CONCERNE LA CARACTERISATION MOLECULAIRE ET FONCTIONNELLE D'UN NOUVEAU GENE DE DROSOPHILA MELANOGASTER, ESSENTIEL AU DEVELOPPEMENT CORRECT DES APPENDICES DE LA MOUCHE, LE GENE TWISTED LEG (TWL). CE GENE CODE POUR UNE PROTEINE PRESENTANT DE FORTES HOMOLOGIES AVEC LES PROTEINES HNRNP K DE VERTEBRES. LA PROTEINE TWL EST DETECTEE TOUT AU LONG DU DEVELOPPEMENT DANS LE NOYAU DES CELLULES, A L'EXCEPTION DES CELLULES POLAIRES OU ELLE EST LOCALISEE DANS LE CYTOPLASME. LES MUTATIONS PERTE DE FONCTION DU GENE TWL REVELENT QUE CE GENE EST NECESSAIRE, MAIS NON ESSENTIEL, A L'EMBRYOGENESE NORMALE. EN REVANCHE, LA FONCTION TWL#+ EST CRITIQUE POUR LE DEVELOPPEMENT CORRECT DES APPENDICES ADULTES. EN SON ABSENCE, CES STRUCTURES, AINSI QUE LES DISQUES IMAGINAUX DONT ELLES DERIVENT, ONT UNE TAILLE REDUITE RESULTANT D'UN DEFAUT DE DIVISIONS CELLULAIRES. DES TRANSGENES EXPRIMANT LA PROTEINE HNRNP K DE DROSOPHILE OU HUMAINE SONT CAPABLES DE SAUVER LE PHENOTYPE D'UN MUTANT HYPOMORPHE DE TWL, MONTRANT LA SIMILARITE FONCTIONNELLE DE CES DEUX PROTEINES IN VIVO. DE PLUS, LES MUTATIONS TWL INTERAGISSENT GENETIQUEMENT AVEC DES MUTATIONS DE DEUX GENES IMPORTANTS POUR LA PROGRESSION DU CYCLE CELLULAIRE, DE2F ET DMCDC2C. CES RESULTATS MONTRENT QUE LA PROLIFERATION DES CELLULES IMAGINALES EST PARTICULIEREMENT SENSIBLE A L'ABSENCE DE LA PROTEINE TWL. DANS LA DEUXIEME PARTIE DU TRAVAIL NOUS AVONS ETUDIE LES EFFETS DE LA SUREXPRESSION DANS LES DISQUES IMAGINAUX DE LA PROTEINE TWL DE DROSOPHILE ET DE SON HOMOLOGUE HNRNP K HUMAIN. DANS LES REGIONS DES DISQUES OU CES PROTEINES SONT SUREXPRIMEES, ON OBSERVE UNE MORT CELLULAIRE INTENSE QUI SE TRADUIT PAR L'ABSENCE DES STRUCTURES EPIDERMIQUES ADULTES CORRESPONDANTES. LA SUREXPRESSION DE LA PROTEINE ANTIAPOPTOTIQUE P35 DU BACULOVIRUS SUPPRIME LE PHENOTYPE LIE A LA SUREXPRESSION DE TWL, MONTRANT QU'UN EXCES DE PROTEINE HNRNP K INDUIT PRINCIPALEMENT UNE MORT CELLULAIRE PAR APOPTOSE. DANS CES EXPERIENCES, NOUS AVONS MIS EN EVIDENCE UNE REGULATION NEGATIVE DE L'EXPRESSION DU GENE TWL ENDOGENE PAR LA PROTEINE HNRNP K HUMAINE, SUGGERANT QU'IL DOIT EXISTER UN MECANISME D'AUTO-REGULATION NEGATIVE QUI CONTROLE LA QUANTITE DE PROTEINE HNRNP K SYNTHETISEE
Caractérisation des mécanismes impliqués dans la promotion de croissance de la Drosophile par Lactobacillus plantarum by Claire-Emmanuelle Indelicato( )

1 edition published in 2017 in English and held by 1 WorldCat member library worldwide

The identification of novel regulators of autophagy in the L3 Drosophila melanogaster fat body by Marwa Elrefaey( )

1 edition published in 2020 in English and held by 1 WorldCat member library worldwide

Autophagy is an evolutionary conserved cellular process, in which parts of the cytoplasm and organelles are delivered to lysosomes for degradation and recycling. Autophagy has gained immense attention in the past decade, due to its pleiotropic role and implication in a large panel of human pathologies. Thus, autophagy needs to be finely regulated. While cytoplasmic control of autophagy through posttranslational modifications has been extensively studied, nuclear transcriptional regulation of autophagy remains largely uncharacterized. The aim of my PhD project was to further characterize the function of Hox proteins and identify additional transcriptional regulators of autophagy in the fat body. My work identified the transcriptomic changes associated with developmental and starvation induced autophagy. Focusing on changes impacting transcription factor encoding genes, I uncovered Slbo as novel transcriptional regulator of autophagy. Slbo was found to act as an autophagy inducer, and to be expressed in a temporally complementary expression pattern to Hox proteins, which were previously established as autophagy repressors. The obtained results collectively allow the conclusion that a tandem of transcriptional activator/repressor times developmental autophagy. Additionally, I investigated the processes that differ in developmentally-programmed and starvation-induced autophagy and further investigated Hox function in the larval fat body. Novel Hox targets were identified, providing further insights into Hox control of autophagy, in addition to highlighting that Hox functions in the fat body are not limited to autophagy control
Réponse immunitaire de la drosophile à la guêpe endoparasitoïde Leptopilina boulardi : caractérisation d'une réaction de résistance by Chami Kim( )

1 edition published in 2019 in English and held by 1 WorldCat member library worldwide

Drosophila melanogaster is a main model in biology, notably immunity and evolution. Although the Drosophila innate immune processes to fight bacteria and fungi have largely been explored, less is known of the defence against endoparasitoid wasps whose successful development inside the insect host leads to its death. One of the most studied Drosophila - parasitoid wasp interaction involves Leptopilina boulardi that lays eggs inside host larvae and develop at their expense. Once the parasitoid egg has been recognized as a foreign invader, the Drosophila larva can mount a successful immune response, the encapsulation: the egg is surrounded by several layers of hemocytes and there is an increase of a specific types of hemocytes, the lamellocytes. The so-formed capsule is melanised and there is formation of reactive oxygen species, which kills the parasitoid. Alternatively, the immune response can be circumvented thanks to the venom components injected by the female wasp together with the egg. Using two Drosophila strains, resistant and susceptible to L. boulardi, which differ only in a region of chromosome 2R containing a major resistance gene. The resistance was found to be monogenic, with two alleles, the resistance allele being dominant (Rlb+>Rlb). The team previously identified edl/mae (R and S alleles) as a candidate gene. Mae (Modulator of the Activity of ETS) or edl (ETS-domain lacking) was described as a mediator of specific transcription factors of the ETS (E26 transformation-specific) family in Drosophila. Mae interacts with transcription factors trough a SAM (Steril Alpha Domain), a protein - protein interaction domain. Mae is known to regulate yan and pnt P2 transcription factors during the eye development and yan and pnt P2 appear to have a role during haematopoiesis. The objectives of my thesis were to decipher the possible role of edl/mae and identify the molecular and cellular events leading to success or failure of encapsulation. I used various approaches from fly genetics to flow cytometry. The involvement of edl/mae in Drosophila resistance was confirmed by using overexpression and interference of mae expression. The overexpression of the resistant allele in a susceptible background leads to a resistant phenotype. The interference of the expression of the susceptible allele results in an increased rate of parasitoid encapsulation. At the cellular level, an increased in the number of hemocytes after parasitism occurred earlier in the resistant strain than in the susceptible strain. It was also observed that the hematopoietic lymph gland of the resistant larvae busted before the one of the susceptible larvae. At the molecular level, potential interactants of mae were identified in silico and 2 were tested using interference of their expression which led to observing an increase of encapsulation. Overall, a key player in the resistance mechanism of the Drosophila to the parasitic wasp have been identify during this work and it lays the path for future work on regulation mechanism of the response at the molecular level
Effets des bioinsecticides à base de Bacillus thuringiensis sur la physiologie intestinale de la Drosophile by Rihab Loudhaief( )

1 edition published in 2016 in French and held by 1 WorldCat member library worldwide

The digestive tract is continuously subjected to multiple aggressions through virus, bacteria, toxins and chemicals mixed in the feed. Therefore the gut lining has established a mechanism of replenishment in order to maintain the physiological function of the organ called the gut homeostasis. Although the deleterious impact of acute poisoning can be overcome by the defense capacity and regeneration of the gut mucosa, prolonged or repeated intoxication can impair its homeostasis. Among the aggressors hidden in the feed, there is the bacterium Bacillus thuringiensis (Bt). Bt is worldwide used as bioinsecticide. Indeed the multitude of Bt strains produces a broad range of crystalline toxins, named Cry toxins, which certain have been selected in organic farming owing to their lethal properties against specific pests. Because of incentive programs for sustainable development, the use of Bt bioinsecticides as an alternative to chemical pesticides will further increase in the next decades. Although the specificity of the acute toxicity of Cry toxins has been proved since many years, data are scarce on adverse effects that could result from chronic exposure. The question now is how far non-target organisms will be potentially impacted by the resulting augmentation of the Bt bacterium and its Cry toxins in the environment. To answer this challenge, I used Drosophila (a non-target organism) to study the impacts of Bt bioinsecticides on the gut physiology because 1/ the digestive tract is the main entrance for feed contaminated by Bt bioinsecticides and 2/ Bt and its toxins are known to impair the gut epithelium of sensitive pests
Détection et régulation du peptidoglycane lors de la réponse antibactérienne chez la Drosophile by Florence Capo( )

1 edition published in 2017 in French and held by 1 WorldCat member library worldwide

Bacterial interactions with the host epithelium can lead to the establishment of a tolerance regarding commensal bacteria or to the triggering of an immune response to eliminate pathogenic bacteria. The detection of bacteria is an essential step in the orientation of this response. In contrast to mammals, the bacteria recognition in Drosophila is mainly based on the detection of a bacterial wall component, peptidoglycan (PG), by a family of receptors, the PeptidoGlycan Recognition Receptors (PGRP). In Drosophila, the PG of extracellular intestinal bacteria can enter the enterocytes and also cross the intestinal epithelium. In the intestine, the detection of PG is regionalized and involves, depending on the domains, two distinct PGRP receptors (PGRP-LC and PGRP-LE). The activation of these PGRPs leads to the activation of the same NF-kB signaling pathway and triggers the production of antimicrobial peptides. The over-activation of this pathway can be harmful to the host, and therefore its intensity is controlled by PGRP proteins which have an enzymatic activity that degrades the elicitor activity of PG.During my thesis, I have generated tools to study the dual mode of PG detection in the intestine. I also tested whether the carriers of the SLC15 family were involved in PG cell trafficking. Part of my thesis was also devoted to clarify the role of catalytic PGRPs in the immune response
 
Audience Level
0
Audience Level
1
  Kids General Special  
Audience level: 0.91 (from 0.84 for Contributi ... to 0.99 for La sympath ...)

Languages