WorldCat Identities

Serwane, Friedhelm

Works: 3 works in 6 publications in 1 language and 10 library holdings
Roles: Author, Other
Publication Timeline
Most widely held works by Friedhelm Serwane
Deterministic preparation of a tunable few-fermion system by Friedhelm Serwane( )

4 editions published in 2011 in English and held by 7 WorldCat member libraries worldwide

A fluid-to-solid jamming transition underlies vertebrate body axis elongation by Alessandro Mongera( )

1 edition published in 2018 in English and held by 2 WorldCat member libraries worldwide

Geometrical characterization of fluorescently labelled surfaces from noisy 3D microscopy data( )

1 edition published in 2017 in English and held by 1 WorldCat member library worldwide

Modern fluorescence microscopy enables fast 3D imaging of biological and inert systems alike. In many studies, it is important to detect the surface of objects and quantitatively characterize its local geometry, including its mean curvature. We present a fully automated algorithm to determine the location and curvatures of an object from 3D fluorescence images, such as those obtained using confocal or light-sheet microscopy. The algorithm aims at reconstructing surface labelled objects with spherical topology and mild deformations from the spherical geometry with high accuracy, rather than reconstructing arbitrarily deformed objects with lower fidelity. Using both synthetic data with known geometrical characteristics and experimental data of spherical objects, we characterize the algorithm's accuracy over the range of conditions and parameters typically encountered in 3D fluorescence imaging. We show that the algorithm can detect the location of the surface and obtain a map of local mean curvatures with relative errors typically below 2% and 20%, respectively, even in the presence of substantial levels of noise. Finally, we apply this algorithm to analyse the shape and curvature map of fluorescently labelled oil droplets embedded within multicellular aggregates and deformed by cellular forces
Audience Level
Audience Level
  Kids General Special  
Audience level: 0.94 (from 0.88 for Geometrica ... to 0.97 for Determinis ...)